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- W2980212707 abstract "After Balb/c female mice were primarily immunized by intra-splenic injection of Balb/c male splenocytes, and then by intraperitoneal injection of male minced skin tissue for five times, they mounted a high immune response. The sera collected from immunized female mice were absorbed by splenocytes from non-immunized female mice, and were subjected to sperm cytotoxicity test giving a titer of 1024. NS-1 cells and splenocytes from immunized mice were fused in 50% PEG. The fusion rate was 100%. after being cloned three times by limiting dilution, four clones of hybridomas that could secret monoclonal antibodies were obtained. The specificity of monoclonal antibodies were determined by embryo cytotoxicity test. The titers of monoclonal antibodies detected with the method described above were 1:512, 1:512, 1:256 and 1:128 respectively." @default.
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- W2980212707 title "Prepartion of monoclonal antibodies against H-Y antigen" @default.
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