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- W2980408385 abstract "Aim. To determine the effectiveness of the process of water purification from phenol by microorganisms-destructors in the formation of a polyvidiofilm biofilm on natural and synthetic carriers in a biofilter. Methods. An association of phenol bacterium destructors — Aeromonas ichthiosmia ONU552, Basillus subtilis ONU551, Pseudomonas maltophilia ONU329, Pseudomonas fluorescens ONU328, Pseudomonas cepacia ONU327 was used. Stained biofilms were stained with 1% acridine orange solution. Microscopy of the samples was carried out under a Carl Zeiss fluorescence microscope and a Carl Zeiss, Primo Star light microscope with photo fixation. The concentration of phenol in water was determined by the extraction-photometric method using 4-aminoantipyrin. Results. Using fluorescence microscopy, it was confirmed that bacteria used for purification of water from phenol - destructors formed a biofilm in a biofilter on carriers of different nature – ceramic tubes, mussel valves, peat, zeolite, activated carbon, synthetic media such as VIYA, sand. In laboratory conditions, the effectiveness of the operation of a column-based biofilter of flow-up-stage type with layer-by-layer complex loading of sorbents during purification of phenol-containing water (initial concentration of phenol – 300 mg/l) was confirmed. After 2 hours of the biofilter operation, the degree of water purification was 40% (residual phenol concentration in water 180±17.2 mg/ l), which was associated with phenol sorption on carriers; during biodegradation, it reached 90% (residual phenol concentration in water – 29.5±2.8 mg/l) on the 6th day. In the following days, the efficiency of the biofilter with continuous intake of phenol-contaminated water was at the level of 50–75%, and in the stationary-cyclic mode reached 80–90% (the concentration of phenol in the water varied from 29.5±2.8 to 60±5.7 mg/l). Conclusion. The new microbial consortium forms a biofilm on natural and synthetic filter carriers, which contributes to the effective purification of water from phenol and the flow-through biofilter operation time (up to 2 months) without additional regeneration." @default.
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- W2980408385 date "2019-09-28" @default.
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- W2980408385 title "DESTRUCTION OF PHENOL AT THE FORMATION OF POLYVIDOUS BIOFILM ON NATURAL AND SYNTHETIC CARRIERS IN THE BIOFILTER" @default.
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- W2980408385 doi "https://doi.org/10.18524/2307-4663.2019.2(46).173857" @default.
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