FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2980456469> ?p ?o ?g. }

• W2980456469 endingPage "112676" @default.
• W2980456469 startingPage "112676" @default.
• W2980456469 abstract "An anti-cocaine monoclonal antibody (mAb) designated h2E2 will soon enter clinical trials for the treatment of cocaine abuse disorders. Importantly, this antibody selectively binds cocaine and its active metabolite, cocaethylene, with high affinity, while binding inactive metabolites with substantially lower affinities. Here, we used differential scanning fluorimetry (DSF) to characterize the stability and ligand binding properties of this antibody and its cocaine-binding Fab fragment. The Sypro orange dye commonly used for DSF revealed multiple overlapping thermal protein denaturation transitions for both the mAb and the Fab fragment, making quantitative analysis of ligand binding by thermal stabilization problematic. However, by using the “rotor” dye, DASPMI (4-(4-(dimethylamino)styryl)-N-methylpyridinium iodide), which measures the rotational restriction of the fluorescent dye (as opposed to the Sypro orange dye which measures the hydrophobicity of the dye microenvironment), a simple two state thermal denaturation transition that is stabilized by ligand binding was observed for the h2E2 mAb, enabling Boltzmann fitting and quantitative thermodynamic analysis of the DASPMI DSF mAb cocaine and metabolite binding data. The computed affinities were consistent with ligand binding affinities determined using other techniques. Thus, this novel DASPMI DSF method can simply, inexpensively, and very rapidly generate ligand binding constants for the h2E2 mAb, despite the presence of multiple, overlapping, thermally unfolding protein domains characteristic of all mAbs. This approach is likely applicable to other mAbs currently in use for many research and therapeutic applications." @default.
• W2980456469 created "2019-10-25" @default.
• W2980456469 creator A5000575185 @default.
• W2980456469 creator A5044221159 @default.
• W2980456469 creator A5085789160 @default.
• W2980456469 date "2020-01-01" @default.
• W2980456469 modified "2023-10-18" @default.
• W2980456469 title "A novel differential scanning fluorimetry analysis of a humanized anti-cocaine mAb and its ligand binding characteristics" @default.
• W2980456469 cites W1976370070 @default.
• W2980456469 cites W1991955074 @default.
• W2980456469 cites W1994102999 @default.
• W2980456469 cites W1995923662 @default.
• W2980456469 cites W1996450030 @default.
• W2980456469 cites W2025265305 @default.
• W2980456469 cites W2025912075 @default.
• W2980456469 cites W2033503609 @default.
• W2980456469 cites W2048009945 @default.
• W2980456469 cites W2065162855 @default.
• W2980456469 cites W2090522614 @default.
• W2980456469 cites W2097052487 @default.
• W2980456469 cites W2108305553 @default.
• W2980456469 cites W2151324528 @default.
• W2980456469 cites W2165697122 @default.
• W2980456469 cites W2346068721 @default.
• W2980456469 cites W2462673634 @default.
• W2980456469 cites W2531506904 @default.
• W2980456469 cites W2542605564 @default.
• W2980456469 cites W2569464392 @default.
• W2980456469 cites W2606919915 @default.
• W2980456469 cites W2621387755 @default.
• W2980456469 cites W2724336921 @default.
• W2980456469 cites W2789733790 @default.
• W2980456469 cites W2898336567 @default.
• W2980456469 cites W2906672240 @default.
• W2980456469 cites W2916175049 @default.
• W2980456469 doi "https://doi.org/10.1016/j.jim.2019.112676" @default.
• W2980456469 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6939126" @default.
• W2980456469 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31634480" @default.
• W2980456469 hasPublicationYear "2020" @default.
• W2980456469 type Work @default.
• W2980456469 sameAs 2980456469 @default.
• W2980456469 citedByCount "13" @default.
• W2980456469 countsByYear W29804564692020 @default.
• W2980456469 countsByYear W29804564692021 @default.
• W2980456469 countsByYear W29804564692022 @default.
• W2980456469 countsByYear W29804564692023 @default.
• W2980456469 crossrefType "journal-article" @default.
• W2980456469 hasAuthorship W2980456469A5000575185 @default.
• W2980456469 hasAuthorship W2980456469A5044221159 @default.
• W2980456469 hasAuthorship W2980456469A5085789160 @default.
• W2980456469 hasBestOaLocation W29804564691 @default.
• W2980456469 hasConcept C107824862 @default.
• W2980456469 hasConcept C116569031 @default.
• W2980456469 hasConcept C12554922 @default.
• W2980456469 hasConcept C13965031 @default.
• W2980456469 hasConcept C159654299 @default.
• W2980456469 hasConcept C170493617 @default.
• W2980456469 hasConcept C185592680 @default.
• W2980456469 hasConcept C203014093 @default.
• W2980456469 hasConcept C2776923230 @default.
• W2980456469 hasConcept C2777477808 @default.
• W2980456469 hasConcept C2780283098 @default.
• W2980456469 hasConcept C542903549 @default.
• W2980456469 hasConcept C55493867 @default.
• W2980456469 hasConcept C71240020 @default.
• W2980456469 hasConcept C86803240 @default.
• W2980456469 hasConceptScore W2980456469C107824862 @default.
• W2980456469 hasConceptScore W2980456469C116569031 @default.
• W2980456469 hasConceptScore W2980456469C12554922 @default.
• W2980456469 hasConceptScore W2980456469C13965031 @default.
• W2980456469 hasConceptScore W2980456469C159654299 @default.
• W2980456469 hasConceptScore W2980456469C170493617 @default.
• W2980456469 hasConceptScore W2980456469C185592680 @default.
• W2980456469 hasConceptScore W2980456469C203014093 @default.
• W2980456469 hasConceptScore W2980456469C2776923230 @default.
• W2980456469 hasConceptScore W2980456469C2777477808 @default.
• W2980456469 hasConceptScore W2980456469C2780283098 @default.
• W2980456469 hasConceptScore W2980456469C542903549 @default.
• W2980456469 hasConceptScore W2980456469C55493867 @default.
• W2980456469 hasConceptScore W2980456469C71240020 @default.
• W2980456469 hasConceptScore W2980456469C86803240 @default.
• W2980456469 hasLocation W29804564691 @default.
• W2980456469 hasLocation W29804564692 @default.
• W2980456469 hasLocation W29804564693 @default.
• W2980456469 hasLocation W29804564694 @default.
• W2980456469 hasOpenAccess W2980456469 @default.
• W2980456469 hasPrimaryLocation W29804564691 @default.
• W2980456469 hasRelatedWork W1488931471 @default.
• W2980456469 hasRelatedWork W1968069322 @default.
• W2980456469 hasRelatedWork W1980377213 @default.
• W2980456469 hasRelatedWork W1982397424 @default.
• W2980456469 hasRelatedWork W2008544142 @default.
• W2980456469 hasRelatedWork W2051779359 @default.
• W2980456469 hasRelatedWork W2052493351 @default.
• W2980456469 hasRelatedWork W2128378535 @default.
• W2980456469 hasRelatedWork W2949568156 @default.
• W2980456469 hasRelatedWork W4232435816 @default.
• W2980456469 hasVolume "476" @default.

• next