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- W2980491301 abstract "Presented here is the exploitation of synchrotron infrared spectromicroscopy to evaluate the feasibility of monitoring the cellular uptake of rhenium-tris-carbonyl-tagged (Re(CO) 3 ) lipophilic chains in living cells. To this aim, an in-house thermostated microfluidic device was used to limit water absorption while keeping cells alive. Indeed, cells showed a high survival rate in the microfluidic device over the course of the experiment, proving the short-term biocompatibility of the device. We recorded spectra of single, living, fully hydrated breast cancer MDA-MB231 cells and could follow the penetration of the rhenium complexes for up to 2 h. Despite the strong variations observed in the uptake kinetics between individual cells, the Re(CO) 3 complex was traced inside the cells at low concentration and shown to enter them on the hour time scale by active transport." @default.
- W2980491301 created "2019-10-25" @default.
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- W2980491301 date "2019-10-16" @default.
- W2980491301 modified "2023-10-14" @default.
- W2980491301 title "Monitoring the Kinetics of the Cellular Uptake of a Metal Carbonyl Conjugated with a Lipidic Moiety in Living Cells Using Synchrotron Infrared Spectromicroscopy" @default.
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- W2980491301 doi "https://doi.org/10.1177/0003702819877260" @default.
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