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- W2980493134 abstract "Pre-analytical handling of CSF needs to be optimized before widespread clinical implementation of Aβ measurements. Aβ42 and Aβ40 were quantified with EUROIMMUN and MSD immunoassays. CSF analyzed within hours after collection into a LoBind tube was used as standard of truth. CSF Aβ was stable for 72 hours at RT, 1 week at 4°Cand at least 2 weeks at -20°C and -80°C. Centrifugation of non-blood-contaminated CSF, or mixing of the samples, did not affect Aβ levels. Addition of blood to CSF followed by storage at RT, led to a dose- and time-dependent decrease inAβ42 and Aβ40, while Aβ42/Aβ40 did not change. The effects of blood contamination were mitigated by centrifugation and storage at 4°C or -20°C. Aβ levels did not differ between the 1st-4th5ml portions of CSF. Based on our findings we propose the following recommendations for a unified pre-analytical protocol: (1) any portion of up to 20ml of CSF (after discarding the first 2ml) could be used for analysis of Aβ; (2) CSF is collected directly into a LoBind tube; (3) centrifugation of CSF after LP is not necessary if no visible blood contamination; (4) fresh CSF samples could be stored at RT for 72h or and at 4°C for 1 week until analyses; (5) mixing of fresh CSF samples before Aβ measurement is not required; (6) CSF samples with visible blood contamination should be centrifuged after LP and stored at 4°C. An alternative approach is to store the CSF collected directly into a LoBind tube at -20°C or -80°C degrees until analyses. The frozen samples should be handled similar to the fresh samples (see above), except that mixing of the thawed samples should be done just before analyses. However, a unified pre-analytical protocol must be decided in consensus by the main stakeholders in the field, which is currently led by the Alzheimer's Association." @default.
- W2980493134 created "2019-10-25" @default.
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- W2980493134 date "2019-07-01" @default.
- W2980493134 modified "2023-09-27" @default.
- W2980493134 title "P1-268: TOWARDS A UNIFIED PROTOCOL FOR HANDLING OF CSF BEFORE β-AMYLOID MEASUREMENTS" @default.
- W2980493134 doi "https://doi.org/10.1016/j.jalz.2019.06.823" @default.
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