Matches in SemOpenAlex for { <https://semopenalex.org/work/W2980495831> ?p ?o ?g. }
- W2980495831 endingPage "392" @default.
- W2980495831 startingPage "381" @default.
- W2980495831 abstract "Abstract Background and Aims Mucosal healing is important in Crohn’s disease therapies. Epithelial homeostasis becomes dysregulated in Crohn’s, with increased permeability, inflammation, and diarrhoea. MicroRNAs are small non-coding RNAs that regulate gene expression and show changes in inflammatory bowel disease. Tumour necrosis factor alpha [TNFα] inhibitor protein 3 is raised in Crohn’s and regulates TNFα-mediated activation of NFκB. We investigated TNFα regulation by microRNA in Crohn’s disease [CD], and studied effects on epithelial permeability and inflammation. Methods Colonic epithelium from CD and healthy donor biopsies was isolated using laser capture microdissection, and microRNA was quantified. Tumour necrosis factor alpha inhibitor protein 3 was characterised immunohistochemically on serial sections. Expression effect of microRNA was confirmed with luciferase reporter assays. Functional barrier permeability studies and innate cytokine release were investigated with cell and explant culture studies. Results MicroRNA23a levels significantly increased in colonic Crohn’s epithelium compared with healthy epithelium. Luciferase reporter assays in transfected epithelial cells confirmed that microRNA23a repressed expression via the 3’ untranslated region of tumour necrosis factor alpha inhibitor protein 3 mRNA, coinciding with increased NFκB-mediated transcription. Immunohistochemical staining of TNFAIP3 protein in colonic biopsies was reduced or absent in adjacent Crohn’s sections, correlating inversely with microRNA23a levels and encompassing some intercohort variation. Overexpression of microRNA23a increased epithelial barrier permeability in a colonic epithelial model and increased inflammatory cytokine release in cultured explant biopsies, mimicking Crohn’s disease characteristics. Conclusions MicroRNA23a overexpression in colonic Crohn’s epithelium represses tumour necrosis factor alpha inhibitor protein 3, enhancing sensitivity to TNFα, with increased intestinal permeability and cytokine release." @default.
- W2980495831 created "2019-10-25" @default.
- W2980495831 creator A5005948755 @default.
- W2980495831 creator A5006723670 @default.
- W2980495831 creator A5046842542 @default.
- W2980495831 creator A5058992944 @default.
- W2980495831 creator A5082049135 @default.
- W2980495831 creator A5085481160 @default.
- W2980495831 date "2019-10-18" @default.
- W2980495831 modified "2023-10-12" @default.
- W2980495831 title "MicroRNA23a Overexpression in Crohn’s Disease Targets Tumour Necrosis Factor Alpha Inhibitor Protein 3, Increasing Sensitivity to TNF and Modifying the Epithelial Barrier" @default.
- W2980495831 cites W1772212485 @default.
- W2980495831 cites W1857468674 @default.
- W2980495831 cites W1885931187 @default.
- W2980495831 cites W1897551156 @default.
- W2980495831 cites W1968069981 @default.
- W2980495831 cites W1977275592 @default.
- W2980495831 cites W1986242902 @default.
- W2980495831 cites W1988163167 @default.
- W2980495831 cites W1993184653 @default.
- W2980495831 cites W1993936200 @default.
- W2980495831 cites W2008497900 @default.
- W2980495831 cites W2008755438 @default.
- W2980495831 cites W2009980852 @default.
- W2980495831 cites W2011236111 @default.
- W2980495831 cites W2013369001 @default.
- W2980495831 cites W2017430900 @default.
- W2980495831 cites W2019558732 @default.
- W2980495831 cites W2020350753 @default.
- W2980495831 cites W2020781390 @default.
- W2980495831 cites W2024346860 @default.
- W2980495831 cites W2027873015 @default.
- W2980495831 cites W2031860350 @default.
- W2980495831 cites W2034236127 @default.
- W2980495831 cites W2039312624 @default.
- W2980495831 cites W2043015183 @default.
- W2980495831 cites W2047398409 @default.
- W2980495831 cites W2055211034 @default.
- W2980495831 cites W2061791452 @default.
- W2980495831 cites W2076588832 @default.
- W2980495831 cites W2076716719 @default.
- W2980495831 cites W2080195364 @default.
- W2980495831 cites W2085429049 @default.
- W2980495831 cites W2086364254 @default.
- W2980495831 cites W2087570816 @default.
- W2980495831 cites W2087966784 @default.
- W2980495831 cites W2092797451 @default.
- W2980495831 cites W2093410766 @default.
- W2980495831 cites W2096014620 @default.
- W2980495831 cites W2097668430 @default.
- W2980495831 cites W2097929425 @default.
- W2980495831 cites W2099029129 @default.
- W2980495831 cites W2103339395 @default.
- W2980495831 cites W2104518242 @default.
- W2980495831 cites W2107744185 @default.
- W2980495831 cites W2122341732 @default.
- W2980495831 cites W2122756053 @default.
- W2980495831 cites W2122935354 @default.
- W2980495831 cites W2126660615 @default.
- W2980495831 cites W2130599353 @default.
- W2980495831 cites W2133968430 @default.
- W2980495831 cites W2134629862 @default.
- W2980495831 cites W2135480784 @default.
- W2980495831 cites W2136845685 @default.
- W2980495831 cites W2142613038 @default.
- W2980495831 cites W2145189896 @default.
- W2980495831 cites W2147954623 @default.
- W2980495831 cites W2150267721 @default.
- W2980495831 cites W2150933705 @default.
- W2980495831 cites W2151871253 @default.
- W2980495831 cites W2151957104 @default.
- W2980495831 cites W2153608030 @default.
- W2980495831 cites W2154302514 @default.
- W2980495831 cites W2157916750 @default.
- W2980495831 cites W2160881772 @default.
- W2980495831 cites W2160899610 @default.
- W2980495831 cites W2161762907 @default.
- W2980495831 cites W2161789287 @default.
- W2980495831 cites W2162904046 @default.
- W2980495831 cites W2164762814 @default.
- W2980495831 cites W2166710590 @default.
- W2980495831 cites W2166842617 @default.
- W2980495831 cites W2167062644 @default.
- W2980495831 cites W2172652502 @default.
- W2980495831 cites W2177396386 @default.
- W2980495831 cites W2300449644 @default.
- W2980495831 cites W2332289047 @default.
- W2980495831 cites W2406496009 @default.
- W2980495831 cites W2567395667 @default.
- W2980495831 cites W2578951881 @default.
- W2980495831 cites W2607252025 @default.
- W2980495831 cites W2768589279 @default.
- W2980495831 cites W2787984929 @default.
- W2980495831 cites W2910065277 @default.
- W2980495831 cites W4211064852 @default.
- W2980495831 cites W566648924 @default.
- W2980495831 cites W645800910 @default.
- W2980495831 doi "https://doi.org/10.1093/ecco-jcc/jjz145" @default.