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- W2980560327 abstract "The central nervous system of the Ciona larva contains only 177 neurons. The precise regulation of neuron subtype-specific morphogenesis and differentiation observed during the formation of this minimal connectome offers a unique opportunity to dissect gene regulatory networks underlying chordate neurodevelopment. Here we compare the transcriptomes of two very distinct neuron types in the hindbrain/spinal cord homolog of Ciona, the Motor Ganglion (MG): the Descending decussating neuron (ddN, proposed homolog of Mauthner Cells in vertebrates) and the MG Interneuron 2 (MGIN2). Both types are invariantly represented by a single bilaterally symmetric left/right pair of cells in every larva. Supernumerary ddNs and MGIN2s were generated in synchronized embryos and isolated by fluorescence-activated cell sorting for transcriptome profiling. Differential gene expression analysis revealed ddN- and MGIN2-specific enrichment of a wide range of genes, including many encoding potential “effectors” of subtype-specific morphological and functional traits. More specifically, we identified the upregulation of centrosome-associated, microtubule-stabilizing/bundling proteins and extracellular guidance cues part of a single intrinsic regulatory program that might underlie the unique polarization of the ddNs, the only descending MG neurons that cross the midline. Consistent with our predictions, CRISPR/Cas9-mediated, tissue-specific elimination of two such candidate effectors, Efcab6-related and Netrin1, impaired ddN polarized axon outgrowth across the midline." @default.
- W2980560327 created "2019-10-25" @default.
- W2980560327 creator A5014613784 @default.
- W2980560327 creator A5030013765 @default.
- W2980560327 creator A5035218326 @default.
- W2980560327 creator A5048657794 @default.
- W2980560327 creator A5061152275 @default.
- W2980560327 creator A5062513598 @default.
- W2980560327 creator A5065470994 @default.
- W2980560327 creator A5086642493 @default.
- W2980560327 date "2020-02-01" @default.
- W2980560327 modified "2023-10-02" @default.
- W2980560327 title "Effector gene expression underlying neuron subtype-specific traits in the Motor Ganglion of Ciona" @default.
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