FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2980572022> ?p ?o ?g. }

• W2980572022 abstract "Abstract Transplastomic potato plants expressing double-stranded RNA (dsRNA) targeted against essential genes of the Colorado potato beetle (CPB) can be lethal to larvae by triggering an RNA interference (RNAi) response. High accumulation levels of dsRNAs in plastids are crucial to confer an efficient RNAi response in the insects. However, whether length and sequence of the dsRNA determine the efficacy of RNAi and/or influence the level of dsRNA accumulation in plastids is not known. Here we compared the RNAi efficacy of different lengths of dsRNA targeted against the CPB β – Actin gene ( ACT ) by feeding in vitro -synthesized dsRNAs to larvae. We show that, while the 60 bp dsRNA induced only a relatively low RNAi response in CPB, dsRNAs of 200 bp and longer caused high mortality and similar larval growth retardation. When the dsRNAs were expressed from the plastid (chloroplast) genome of potato plants, we found that their accumulation levels were correlated with length. dsRNA accumulation levels were positively associated with the observed mortality, suppression of larval growth and suppression of target gene expression. Importantly, transplastomic potato plants expressing the 200 bp dsRNA were better protected from CPB than plants expressing the 297 bp dsRNA, the best-performing line in our previous study. Our results suggest that the length of dsRNAs is an important factor that influences their accumulation levels in plastids and thus determines the strength of the insecticidal RNAi effect. Our findings will aid the design of optimized dsRNA expression constructs for plant protection by plastid-mediated RNAi." @default.
• W2980572022 created "2019-10-25" @default.
• W2980572022 creator A5008826700 @default.
• W2980572022 creator A5031502631 @default.
• W2980572022 creator A5062875328 @default.
• W2980572022 creator A5066311980 @default.
• W2980572022 creator A5075199511 @default.
• W2980572022 creator A5083845228 @default.
• W2980572022 creator A5091105531 @default.
• W2980572022 date "2019-10-21" @default.
• W2980572022 modified "2023-09-27" @default.
• W2980572022 title "Length-dependent accumulation of double-stranded RNAs in plastids affects RNA interference efficiency in the Colorado potato beetle" @default.
• W2980572022 cites W1647075334 @default.
• W2980572022 cites W1971894293 @default.
• W2980572022 cites W1976431857 @default.
• W2980572022 cites W1976879480 @default.
• W2980572022 cites W1985738221 @default.
• W2980572022 cites W1994113364 @default.
• W2980572022 cites W2010677471 @default.
• W2980572022 cites W2021552468 @default.
• W2980572022 cites W2024556021 @default.
• W2980572022 cites W2054363760 @default.
• W2980572022 cites W2073840770 @default.
• W2980572022 cites W2092704486 @default.
• W2980572022 cites W2099824652 @default.
• W2980572022 cites W2107277218 @default.
• W2980572022 cites W2120210898 @default.
• W2980572022 cites W2121440246 @default.
• W2980572022 cites W2122181911 @default.
• W2980572022 cites W2127535576 @default.
• W2980572022 cites W2128412621 @default.
• W2980572022 cites W2152730479 @default.
• W2980572022 cites W2154993535 @default.
• W2980572022 cites W2159448158 @default.
• W2980572022 cites W2160401863 @default.
• W2980572022 cites W2298413610 @default.
• W2980572022 cites W2736891943 @default.
• W2980572022 cites W2750290863 @default.
• W2980572022 cites W2896105415 @default.
• W2980572022 cites W2907693074 @default.
• W2980572022 cites W2920555400 @default.
• W2980572022 cites W2924100339 @default.
• W2980572022 doi "https://doi.org/10.1101/812750" @default.
• W2980572022 hasPublicationYear "2019" @default.
• W2980572022 type Work @default.
• W2980572022 sameAs 2980572022 @default.
• W2980572022 citedByCount "0" @default.
• W2980572022 crossrefType "posted-content" @default.
• W2980572022 hasAuthorship W2980572022A5008826700 @default.
• W2980572022 hasAuthorship W2980572022A5031502631 @default.
• W2980572022 hasAuthorship W2980572022A5062875328 @default.
• W2980572022 hasAuthorship W2980572022A5066311980 @default.
• W2980572022 hasAuthorship W2980572022A5075199511 @default.
• W2980572022 hasAuthorship W2980572022A5083845228 @default.
• W2980572022 hasAuthorship W2980572022A5091105531 @default.
• W2980572022 hasBestOaLocation W29805720221 @default.
• W2980572022 hasConcept C104317684 @default.
• W2980572022 hasConcept C166703698 @default.
• W2980572022 hasConcept C2191507 @default.
• W2980572022 hasConcept C54355233 @default.
• W2980572022 hasConcept C67705224 @default.
• W2980572022 hasConcept C69305403 @default.
• W2980572022 hasConcept C86803240 @default.
• W2980572022 hasConcept C93829228 @default.
• W2980572022 hasConcept C95444343 @default.
• W2980572022 hasConceptScore W2980572022C104317684 @default.
• W2980572022 hasConceptScore W2980572022C166703698 @default.
• W2980572022 hasConceptScore W2980572022C2191507 @default.
• W2980572022 hasConceptScore W2980572022C54355233 @default.
• W2980572022 hasConceptScore W2980572022C67705224 @default.
• W2980572022 hasConceptScore W2980572022C69305403 @default.
• W2980572022 hasConceptScore W2980572022C86803240 @default.
• W2980572022 hasConceptScore W2980572022C93829228 @default.
• W2980572022 hasConceptScore W2980572022C95444343 @default.
• W2980572022 hasLocation W29805720221 @default.
• W2980572022 hasLocation W29805720222 @default.
• W2980572022 hasOpenAccess W2980572022 @default.
• W2980572022 hasPrimaryLocation W29805720221 @default.
• W2980572022 hasRelatedWork W1984104677 @default.
• W2980572022 hasRelatedWork W2017195809 @default.
• W2980572022 hasRelatedWork W2019794323 @default.
• W2980572022 hasRelatedWork W2034014168 @default.
• W2980572022 hasRelatedWork W2110691849 @default.
• W2980572022 hasRelatedWork W2126643686 @default.
• W2980572022 hasRelatedWork W2491374855 @default.
• W2980572022 hasRelatedWork W2791084574 @default.
• W2980572022 hasRelatedWork W2899526179 @default.
• W2980572022 hasRelatedWork W2916363255 @default.
• W2980572022 hasRelatedWork W2969015775 @default.
• W2980572022 hasRelatedWork W3000027631 @default.
• W2980572022 hasRelatedWork W3038576234 @default.
• W2980572022 hasRelatedWork W3128409559 @default.
• W2980572022 hasRelatedWork W3137754583 @default.
• W2980572022 hasRelatedWork W3139252431 @default.
• W2980572022 hasRelatedWork W3149820778 @default.
• W2980572022 hasRelatedWork W3163199567 @default.
• W2980572022 hasRelatedWork W3163979500 @default.
• W2980572022 hasRelatedWork W3169422738 @default.
• W2980572022 isParatext "false" @default.
• W2980572022 isRetracted "false" @default.

• next