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• W2980773903 abstract "Tailor-made cloning solutions are gradually replacing classical cloning methods. Current systems make use of libraries with predefined DNA parts that are joined by Golden-Gate reactions. However, these systems still suffer from specific inflexibilities and the lack of inter-compatibility. Here, we present Flexible Modular Cloning (MoCloFlex) which overcomes this inflexibility by introducing a set of linker- and position-vectors allowing free unit arrangement. Our system, therefore, provides a convenient way to design and build custom plasmids, and iterative assembly of large constructs. To support standardization in synthetic biology, MoCloFlex is compatible with the well-established Modular Cloning standard. Here, we test MoCloFlex form various applications with up to 12 fragments in a single restriction-ligation reaction." @default.
• W2980773903 created "2019-10-25" @default.
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• W2980773903 date "2019-10-17" @default.
• W2980773903 modified "2023-09-27" @default.
• W2980773903 title "MoCloFlex: A Modular Yet Flexible Cloning System" @default.
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• W2980773903 doi "https://doi.org/10.3389/fbioe.2019.00271" @default.
• W2980773903 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6843054" @default.
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