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• W2980781807 abstract "Cerebrospinal fluid (CSF) biomarker concentrations are valuable in the diagnosis of Alzheimer's Disease (AD). CSF β-amyloid 1–42 (Aβ42), is a core biomarker of AD. While analytic variability has been reduced with the advent of automated assays, pre-analytical variability has limited clinical use. The main purpose of this study was to evaluate the effect of patient-specific pre-analytical variables (fasting, inadvertent blood contamination, CSF gradients) on Aβ42 concentrations. Freshly-collected and frozen CSF samples obtained from the Johns Hopkins Center for CSF Disorders were subjected to patient specific protocols. Aβ42 concentrations were measured using Lumipulse G1200 (Fujirebio Diagnostics Inc., Malvern, PA). Fresh and frozen CSF was analyzed post-centrifugation (2000 g, 10 minutes, 5 ± 3°C) with fresh samples analyzed within two hours of collection. Patient fasting was evaluated (n=10) with fasting maintained a minimum of six hours. Prior to breakfast, CSF was collected in two or three 1 ml aliquots and simultaneous blood and CSF glucose measurements were obtained. Post-meal, these procedures were repeated each hour for 3 hours. Patient CSF gradients (n=6) were studied using 20 serial 1 mL CSF aliquots. Blood contamination was evaluated in fresh CSF samples (n=7) spiked with varying levels of whole blood (0.1%, 0.5%, 1%, 2.5%, 5%, 10%). Frozen CSF samples (n=3) were also spiked with varying levels of whole blood (0.25%, 0.5%, 2.5%, 5%) and measured. CSF gradients did not significantly alter Aβ42 concentrations. Percent difference ranged from 0% to 6% using pairwise analysis across gradient aliquots. Aβ42 levels were stable when fresh CSF samples were spiked with up to 2.5% of fresh blood. However, ≥ 0.25% blood contamination in frozen CSF samples significantly decreased Aβ42 concentrations. No significant change was observed in CSF Aβ42 levels in fasting patients with an average % difference and standard deviation of -1±3.8 at 1 hour, -1±4.3 at 2 hours and 1±4.4 at 3 hours post-breakfast. Real world CSF Aβ42 measurement may be impacted by fasting, gradient effects, and inadvertent blood contamination. Our data suggests that these pre-analytical variables do not have significant effects on concentrations of AB42 if fresh samples are processed within 2 hours." @default.
• W2980781807 created "2019-10-25" @default.
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• W2980781807 date "2019-07-01" @default.
• W2980781807 modified "2023-09-28" @default.
• W2980781807 title "P4-476: EFFECT OF PATIENT-SPECIFIC PRE-ANALYTIC VARIABLES ON CSF Aβ42 CONCENTRATIONS MEASURED ON AN AUTOMATED CHEMILUMINESCENT PLATFORM" @default.
• W2980781807 doi "https://doi.org/10.1016/j.jalz.2019.08.022" @default.
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