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• W2980786987 abstract "Metabolic adaptation to the host cell is important for obligate intracellular pathogens such as Chlamydia trachomatis (Ct). Here we infer the flux differences for Ct from proteome and qRT-PCR data by comprehensive pathway modelling. We compare the comparatively inert infectious elementary body (EB) and the active replicative reticulate body (RB) systematically using a genome-scale metabolic model with 321 metabolites and 277 reactions. This did yield 84 extreme pathways based on a published proteomics dataset at three different time points of infection. Validation of predictions was done by quantitative RT-PCR of enzyme mRNA expression at three time points. Ct’s major active pathways are glycolysis, gluconeogenesis, glycerol-phospholipid biosynthesis (support from host acetyl-CoA) and pentose phosphate pathway, while its incomplete TCA and fatty acid biosynthesis are less active. The modelled metabolic pathways are much more active in RB than in EB. Our in silico model suggests that EB and RB utilize folate to generate NAD(P)H using independent pathways. The only low metabolic flux inferred for EB involves mainly carbohydrate metabolism. RB utilizes energy -rich compounds to generate ATP in nucleic acid metabolism. Validation data for the modelling include proteomics experiments (model basis) as well as qRT-PCR confirmation of selected metabolic enzyme mRNA expression differences. The metabolic modelling is made fully available here. Its detailed insights and models on Ct metabolic adaptations during infection are a useful modelling basis for future studies." @default.
• W2980786987 created "2019-10-25" @default.
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• W2980786987 date "2019-10-15" @default.
• W2980786987 modified "2023-10-04" @default.
• W2980786987 title "Comprehensive Flux Modeling of Chlamydia trachomatis Proteome and qRT-PCR Data Indicate Biphasic Metabolic Differences Between Elementary Bodies and Reticulate Bodies During Infection" @default.
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• W2980786987 doi "https://doi.org/10.3389/fmicb.2019.02350" @default.
• W2980786987 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6803457" @default.
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