FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2980876814> ?p ?o ?g. }

• W2980876814 endingPage "772" @default.
• W2980876814 startingPage "766" @default.
• W2980876814 abstract "Objective: To investigate the relationship between miRNA-196b-5p and miRNA-99a-5p expression and autophagy and apoptosis in multiple myeloma cells. Methods: Human myeloma cell line U266 and normal CD138+ plasma cells were selected as the research objects. The subjects were divided into 45 cases of multiple myeloma patients and 40 healthy controls. The expression of miRNA-196b-5p and miRNA-99a-5p was measured by real-time quantitative PCR, and Western blot was used to determine the expression of autophagy related protein LC3-Ⅱ, LC3-Ⅰ, P62, Beclin-1 expression, apoptosis related protein CL caspase3, CL caspase7, Bcl-2, Bax, and TGF-β/Smad pathway associated proteins TGF-β1, Smad2/3, p-Smad3 and Smad7. The cell apoptosis rate was determined by flow cytometry. The correlation between miRNA expression level and clinical characteristics of multiple myeloma patients was analyzed. Results: Compared with normal plasma cells, the expression of miRNA-196b-5p in myeloma cells increased significantly (0.43±0.15 vs 2.44±0.63 or 2.02±0.85, all P<0.001), the expression of miRNA-99a-5p was significantly decreased (1.87±0.61 vs 0.62±0.15 or 0.80±0.33, P<0.001), LC3-Ⅱ/LC3-Ⅰ increased significantly (P<0.05), Beclin-1 expression increased significantly (P<0.05), P62 expression decreased significantly (P<0.05). The expression of Bax, CL caspase3 and CL caspase7 decreased significantly (P<0.05), and the expression of Bcl-2 increased significantly (P<0.05) and apoptosis rate significantly decreased (P<0.05). After transfected with miRNA-196b-5p mimic or miRNA-99a-5p inhibitor, the LC3-Ⅱ/LC3-Ⅰ of CD138+ plasma cells increased significantly (P<0.05), the expression of Beclin-1 increased significantly (P<0.05), P62 expression decreased significantly (P<0.05), and the apoptosis rate significantly decreased (P<0.05). However, after autophagy inhibitor of 3-MA was administered, the apoptotic rate of the above reaction system did not change significantly (P>0.05). The expression of miRNA-196b-5p and miRNA-99a-5p was significantly correlated with DS and ISS stage in multiple myeloma patients (P<0.05). Conclusion: miRNA-196b-5p and miRNA-99a-5p are closely related to the clinical characteristics of patients with multiple myeloma. The overexpression of miRNA-196b-5p and down regulation of miRNA-99a-5p could inhibit the apoptosis of myeloma cells by up regulation of autophagy, and the mechanism is related to the activation of the TGF-β/Smad signaling pathway.目的： 探讨多发性骨髓瘤（MM）细胞miRNA-196b-5p和miRNA-99a-5p表达与自噬和凋亡的关系。 方法： 以人MM细胞株U266细胞及45例MM患者瘤细胞为研究对象，以40例健康体检者为对照，采用实时定量PCR法测定细胞中miRNA-196b-5p和miRNA-99a-5p表达水平，采用Western blot法测定自噬相关蛋白（LC3-Ⅱ、LC3-Ⅰ、P62、Beclin-1）、凋亡相关分子（CL caspase3、CL caspase7、Bcl-2、Bax）和TGF-β/Smad通路相关蛋白（TGF-β1、Smad2/3、p-Smad3、Smad7）表达水平，采用流式细胞术测定细胞凋亡率，分析miRNA表达水平与MM患者临床特征的相关性。 结果： ①与正常浆细胞比较，U266细胞、MM患者瘤细胞的miRNA-196b-5p表达显著增加（0.43±0.15对2.44±0.63、2.02±0.85，P值均<0.001），miRNA-99a-5p表达显著降低（1.87±0.61对0.62±0.15、0.80±0.33，P值均<0.001），LC3-Ⅱ/LC3-Ⅰ、Beclin-1、Bcl-2表达显著增加，P62、Bax、CL caspase3、CL caspase7表达显著降低，细胞凋亡率降低（P值均<0.05）。②正常CD138(+)浆细胞分别转染miRNA-196b-5p mimic或miRNA-99a-5p inhibitor 48 h后，与未转染对照组比较，LC3-Ⅱ/LC3-Ⅰ、Beclin-1、TGF-β1、p-Smad3表达显著增高，P62、Smad7表达显著降低，细胞凋亡率减少（P值均<0.05）；上述反应体系加入自噬抑制剂3-甲基腺嘌呤后，细胞凋亡率未见明显改变（P值均>0.05）。③miRNA-196b-5p和miRNA-99a-5p表达与MM患者DS分期（P值分别为0.004、0.012）、ISS分期显著相关（P值均为0.001）。 结论： miRNA-196b-5p和miRNA-99a-5p表达与MM患者临床特征密切相关，miRNA-196b-5p过表达和miRNA-99a-5p表达下调通过上调自噬水平抑制骨髓瘤细胞凋亡，其机制与活化TGF-β/Smad信号通路有关。." @default.
• W2980876814 created "2019-10-25" @default.
• W2980876814 creator A5049937552 @default.
• W2980876814 creator A5061275018 @default.
• W2980876814 creator A5066707799 @default.
• W2980876814 creator A5070838130 @default.
• W2980876814 creator A5074852608 @default.
• W2980876814 creator A5087775329 @default.
• W2980876814 date "2018-09-14" @default.
• W2980876814 modified "2023-10-16" @default.
• W2980876814 title "[Association of miRNA-196b-5p and miRNA-99a-5p with autophagy and apoptosis in multiple myeloma cells]." @default.
• W2980876814 cites W1960196841 @default.
• W2980876814 cites W1964914496 @default.
• W2980876814 cites W1993469143 @default.
• W2980876814 cites W1993643473 @default.
• W2980876814 cites W2040722278 @default.
• W2980876814 cites W2041161710 @default.
• W2980876814 cites W2045267701 @default.
• W2980876814 cites W2068287405 @default.
• W2980876814 cites W2075959152 @default.
• W2980876814 cites W2093027716 @default.
• W2980876814 cites W2094636384 @default.
• W2980876814 cites W2120644885 @default.
• W2980876814 cites W2122212799 @default.
• W2980876814 cites W2214896346 @default.
• W2980876814 cites W2288701630 @default.
• W2980876814 cites W2293413684 @default.
• W2980876814 cites W2325506966 @default.
• W2980876814 cites W2395563932 @default.
• W2980876814 cites W2569800935 @default.
• W2980876814 cites W2582797782 @default.
• W2980876814 cites W2591333313 @default.
• W2980876814 cites W2618114404 @default.
• W2980876814 cites W2737128405 @default.
• W2980876814 cites W4293307235 @default.
• W2980876814 doi "https://doi.org/10.3760/cma.j.issn.0253-2727.2018.09.013" @default.
• W2980876814 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7342263" @default.
• W2980876814 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30369190" @default.
• W2980876814 hasPublicationYear "2018" @default.
• W2980876814 type Work @default.
• W2980876814 sameAs 2980876814 @default.
• W2980876814 citedByCount "3" @default.
• W2980876814 countsByYear W29808768142019 @default.
• W2980876814 countsByYear W29808768142021 @default.
• W2980876814 countsByYear W29808768142023 @default.
• W2980876814 crossrefType "journal-article" @default.
• W2980876814 hasAuthorship W2980876814A5049937552 @default.
• W2980876814 hasAuthorship W2980876814A5061275018 @default.
• W2980876814 hasAuthorship W2980876814A5066707799 @default.
• W2980876814 hasAuthorship W2980876814A5070838130 @default.
• W2980876814 hasAuthorship W2980876814A5074852608 @default.
• W2980876814 hasAuthorship W2980876814A5087775329 @default.
• W2980876814 hasConcept C104317684 @default.
• W2980876814 hasConcept C127561419 @default.
• W2980876814 hasConcept C145059251 @default.
• W2980876814 hasConcept C153911025 @default.
• W2980876814 hasConcept C16685009 @default.
• W2980876814 hasConcept C190283241 @default.
• W2980876814 hasConcept C203014093 @default.
• W2980876814 hasConcept C203522944 @default.
• W2980876814 hasConcept C2776364478 @default.
• W2980876814 hasConcept C2776415932 @default.
• W2980876814 hasConcept C502942594 @default.
• W2980876814 hasConcept C54009773 @default.
• W2980876814 hasConcept C54355233 @default.
• W2980876814 hasConcept C553184892 @default.
• W2980876814 hasConcept C71924100 @default.
• W2980876814 hasConcept C81885089 @default.
• W2980876814 hasConcept C86803240 @default.
• W2980876814 hasConceptScore W2980876814C104317684 @default.
• W2980876814 hasConceptScore W2980876814C127561419 @default.
• W2980876814 hasConceptScore W2980876814C145059251 @default.
• W2980876814 hasConceptScore W2980876814C153911025 @default.
• W2980876814 hasConceptScore W2980876814C16685009 @default.
• W2980876814 hasConceptScore W2980876814C190283241 @default.
• W2980876814 hasConceptScore W2980876814C203014093 @default.
• W2980876814 hasConceptScore W2980876814C203522944 @default.
• W2980876814 hasConceptScore W2980876814C2776364478 @default.
• W2980876814 hasConceptScore W2980876814C2776415932 @default.
• W2980876814 hasConceptScore W2980876814C502942594 @default.
• W2980876814 hasConceptScore W2980876814C54009773 @default.
• W2980876814 hasConceptScore W2980876814C54355233 @default.
• W2980876814 hasConceptScore W2980876814C553184892 @default.
• W2980876814 hasConceptScore W2980876814C71924100 @default.
• W2980876814 hasConceptScore W2980876814C81885089 @default.
• W2980876814 hasConceptScore W2980876814C86803240 @default.
• W2980876814 hasIssue "9" @default.
• W2980876814 hasLocation W29808768141 @default.
• W2980876814 hasOpenAccess W2980876814 @default.
• W2980876814 hasPrimaryLocation W29808768141 @default.
• W2980876814 hasRelatedWork W2351013616 @default.
• W2980876814 hasRelatedWork W2351060232 @default.
• W2980876814 hasRelatedWork W2373529589 @default.
• W2980876814 hasRelatedWork W2380107697 @default.
• W2980876814 hasRelatedWork W2386311237 @default.
• W2980876814 hasRelatedWork W2903642138 @default.
• W2980876814 hasRelatedWork W3030315913 @default.
• W2980876814 hasRelatedWork W3094035277 @default.

• next