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• W2980930018 abstract "Single-molecule spectral imaging is a powerful technique for simultaneous study of biological samples labeled by multiple fluorophores. We have developed a robust prism-based single-molecule spectral tracking technique based on a dual-objective fluorescence–reflection system with tunable spectral dispersion (DOFR-SD). The system can capture the spatial position of a single molecule in real time, and rather than splitting photons into two channels its intrinsic spectral characteristics can also be captured without compromising the precision of the (x, y) position. Thus, the DOFR-SD technique is suitable for tracking the spectral variation of single particles in living cells and for multicolor coding." @default.
• W2980930018 created "2019-10-25" @default.
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• W2980930018 date "2019-10-17" @default.
• W2980930018 modified "2023-10-16" @default.
• W2980930018 title "Spectroscopic fluorescent tracking of a single molecule in a live cell with a dual-objective fluorescent reflection microscope" @default.
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• W2980930018 doi "https://doi.org/10.7567/1882-0786/ab4b16" @default.
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