FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2980996914> ?p ?o ?g. }

• W2980996914 abstract "Abstract The autofluorescent coenzyme nicotinamide adenine dinucleotide (NADH) and its phosphorylated form (NADPH) are major determinants of cellular redox balance. Both their fluorescence intensities and lifetimes are extensively used as label-free readouts in cellular metabolic imaging studies. Here, we introduce fluorescence blinking of NAD(P)H, as an additional, orthogonal readout in such studies. Blinking of fluorophores and their underlying dark state transitions are specifically sensitive to redox conditions and oxygenation, parameters of particular relevance in cellular metabolic studies. We show that such dark state transitions in NAD(P)H can be quantified via the average fluorescence intensity recorded upon modulated one-photon excitation, so-called transient state (TRAST) monitoring. Thereby, transitions in NAD(P)H, previously only accessible from elaborate spectroscopic cuvette measurements, can be imaged at subcellular resolution in live cells. We then demonstrate that these transitions can be imaged with a standard laser-scanning confocal microscope and two-photon excitation, in parallel with regular fluorescence lifetime imaging (FLIM). TRAST imaging of NAD(P)H was found to provide additional, orthogonal information to FLIM and allows altered oxidative environments in cells treated with a mitochondrial un-coupler or cyanide to be clearly distinguished. We propose TRAST imaging as a straightforward and widely applicable modality, extending the range of information obtainable from cellular metabolic imaging of NAD(P)H fluorescence." @default.
• W2980996914 created "2019-10-25" @default.
• W2980996914 creator A5004925265 @default.
• W2980996914 creator A5013002722 @default.
• W2980996914 creator A5049966019 @default.
• W2980996914 creator A5072416757 @default.
• W2980996914 date "2019-10-21" @default.
• W2980996914 modified "2023-10-14" @default.
• W2980996914 title "Local redox conditions in cells imaged via non-fluorescent transient states of NAD(P)H" @default.
• W2980996914 cites W1491342873 @default.
• W2980996914 cites W1892152977 @default.
• W2980996914 cites W1963667377 @default.
• W2980996914 cites W1970554352 @default.
• W2980996914 cites W1976786825 @default.
• W2980996914 cites W1981928793 @default.
• W2980996914 cites W1983264189 @default.
• W2980996914 cites W1987464952 @default.
• W2980996914 cites W1988328429 @default.
• W2980996914 cites W1993759736 @default.
• W2980996914 cites W1994149778 @default.
• W2980996914 cites W1995417258 @default.
• W2980996914 cites W2005269675 @default.
• W2980996914 cites W2006744863 @default.
• W2980996914 cites W2009496186 @default.
• W2980996914 cites W2015072959 @default.
• W2980996914 cites W2020690076 @default.
• W2980996914 cites W2022922186 @default.
• W2980996914 cites W2025568741 @default.
• W2980996914 cites W2029171692 @default.
• W2980996914 cites W2033221341 @default.
• W2980996914 cites W2040815295 @default.
• W2980996914 cites W2045776685 @default.
• W2980996914 cites W2049126768 @default.
• W2980996914 cites W2051823351 @default.
• W2980996914 cites W2061464631 @default.
• W2980996914 cites W2068998399 @default.
• W2980996914 cites W2070341726 @default.
• W2980996914 cites W2072244905 @default.
• W2980996914 cites W2077879155 @default.
• W2980996914 cites W2079780189 @default.
• W2980996914 cites W2084510496 @default.
• W2980996914 cites W2085800696 @default.
• W2980996914 cites W2087294008 @default.
• W2980996914 cites W2089975180 @default.
• W2980996914 cites W2100823746 @default.
• W2980996914 cites W2111920179 @default.
• W2980996914 cites W2119795324 @default.
• W2980996914 cites W2123095100 @default.
• W2980996914 cites W2129460817 @default.
• W2980996914 cites W2129827453 @default.
• W2980996914 cites W2142635246 @default.
• W2980996914 cites W2168175751 @default.
• W2980996914 cites W2294018174 @default.
• W2980996914 cites W2298361824 @default.
• W2980996914 cites W2345381093 @default.
• W2980996914 cites W2444674706 @default.
• W2980996914 cites W2475612411 @default.
• W2980996914 cites W2503953104 @default.
• W2980996914 cites W2538315435 @default.
• W2980996914 cites W2770434908 @default.
• W2980996914 cites W2777598998 @default.
• W2980996914 cites W2897619192 @default.
• W2980996914 cites W2951463143 @default.
• W2980996914 doi "https://doi.org/10.1038/s41598-019-51526-w" @default.
• W2980996914 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6803634" @default.
• W2980996914 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31636326" @default.
• W2980996914 hasPublicationYear "2019" @default.
• W2980996914 type Work @default.
• W2980996914 sameAs 2980996914 @default.
• W2980996914 citedByCount "8" @default.
• W2980996914 countsByYear W29809969142021 @default.
• W2980996914 countsByYear W29809969142022 @default.
• W2980996914 crossrefType "journal-article" @default.
• W2980996914 hasAuthorship W2980996914A5004925265 @default.
• W2980996914 hasAuthorship W2980996914A5013002722 @default.
• W2980996914 hasAuthorship W2980996914A5049966019 @default.
• W2980996914 hasAuthorship W2980996914A5072416757 @default.
• W2980996914 hasBestOaLocation W29809969141 @default.
• W2980996914 hasConcept C120665830 @default.
• W2980996914 hasConcept C121332964 @default.
• W2980996914 hasConcept C12554922 @default.
• W2980996914 hasConcept C178790620 @default.
• W2980996914 hasConcept C181199279 @default.
• W2980996914 hasConcept C185592680 @default.
• W2980996914 hasConcept C197957613 @default.
• W2980996914 hasConcept C2776901170 @default.
• W2980996914 hasConcept C47785574 @default.
• W2980996914 hasConcept C55493867 @default.
• W2980996914 hasConcept C55904794 @default.
• W2980996914 hasConcept C57477423 @default.
• W2980996914 hasConcept C57600042 @default.
• W2980996914 hasConcept C75473681 @default.
• W2980996914 hasConcept C75520062 @default.
• W2980996914 hasConcept C86803240 @default.
• W2980996914 hasConcept C91881484 @default.
• W2980996914 hasConceptScore W2980996914C120665830 @default.
• W2980996914 hasConceptScore W2980996914C121332964 @default.
• W2980996914 hasConceptScore W2980996914C12554922 @default.
• W2980996914 hasConceptScore W2980996914C178790620 @default.
• W2980996914 hasConceptScore W2980996914C181199279 @default.

• next