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- W2981030237 endingPage "110589" @default.
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- W2981030237 abstract "Built upon our interest in illustrating the complexity of protein adsorption onto chromatographic supports and to understand the rule of nonspecific interactions in the ion exchange adsorption process, a traditional model system (lysozyme - carboxymethyl cellulose) was used to determine the charge influence during biomolecule adsorption. Flow microcalorimetry (FMC) was exploit as a dynamic technique that provides adsorption and desorption heat signals for a specific system, permitting an improved understanding of the driving forces and mechanisms involved during the interaction. For this purpose, measurements were made at pH 8 at both absence and presence of salt (NaCl 50 mM) and compared with previous studies performed at pH 5. Distinct FMC profiles were observed regarding pH. For most of the experiments, two exothermic heat signals are observed at pH 8 while at pH 5 one endothermic and one exothermic peak are shown. This difference was justified with a less energy demanding for desolvation at pH 8. Lysozyme adsorption was shown to be a multi-step process involving desolvation, primary protein adsorption and secondary adsorption after reorientation with distinct contributions to the overall energy. At pH 8, the exothermic contribution to the adsorption process is lower compared to pH 5, which is justified by the lower charge density that lysozyme presents at pH 8 compared to pH 5." @default.
- W2981030237 created "2019-10-25" @default.
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- W2981030237 date "2020-01-01" @default.
- W2981030237 modified "2023-09-26" @default.
- W2981030237 title "Calorimetric approach to understand pH and salt influence on the adsorption mechanism of lysozyme to a traditional cation exchanger" @default.
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- W2981030237 doi "https://doi.org/10.1016/j.colsurfb.2019.110589" @default.
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