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• W2981036624 abstract "Abstract Optical mapping of linearized DNA molecules is a promising new technology for sequence assembly and scaffolding, large structural variant detection, and diagnostics. This is currently achieved either using nanochannel confinement or by stretching single DNA molecules on a solid surface. While the first method necessitates DNA labelling before linearization, the latter allows for modification post-linearization, thereby affording increased process flexibility. Each method is constrained by various physical and chemical limitations. One of the most common techniques for linearization of DNA uses a hydrophobic surface and a receding meniscus, termed molecular combing. Here, we report the development of a microfabricated surface that can not only comb the DNA molecules efficiently but also provides for sequence-specific enzymatic fluorescent DNA labelling. By modifying a glass surface with two contrasting functionalities, such that DNA binds selectively to one of the two regions, we can control DNA extension, which is known to be critical for sequence-recognition by an enzyme. Moreover, the surface modification provides enzymatic access to the DNA backbone, as well as minimizing non-specific fluorescent dye adsorption. These enhancements make the designed surface suitable for large-scale and high-resolution single DNA molecule studies." @default.
• W2981036624 created "2019-10-25" @default.
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• W2981036624 date "2019-10-21" @default.
• W2981036624 modified "2023-10-12" @default.
• W2981036624 title "A micropatterned substrate for on-surface enzymatic labelling of linearized long DNA molecules" @default.
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• W2981036624 doi "https://doi.org/10.1038/s41598-019-51507-z" @default.
• W2981036624 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6803683" @default.
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• W2981036624 hasPublicationYear "2019" @default.
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