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- W2981128682 abstract "JC virus (JCV) Agnoprotein (Agno) plays critical roles in successful completion of the viral replication cycle. Understanding its regulatory roles requires a complete map of JCV-host protein interactions. Here, we report the first Agno interactome with host cellular targets utilizing “Two-Strep-Tag” affinity purification system coupled with mass spectroscopy (AP/MS). Proteomics data revealed that Agno primarily targets 501 cellular proteins, most of which contain “coiled-coil” motifs. Agno-host interactions occur in several cellular networks including those involved in protein synthesis and degradation; and cellular transport; and in organelles, including mitochondria, nucleus and ER-Golgi network. Among the Agno interactions, Rab11B, Importin and Crm-1 were first validated biochemically and further characterization was done for Crm-1, using a HIV-1 Rev-M10-like Agno mutant (L33D + E34L), revealing the critical roles of L33 and E34 residues in Crm-1 interaction. This comprehensive proteomics data provides new foundations to unravel the critical regulatory roles of Agno during the JCV life cycle. • Agnoprotein is one of the regulatory proteins of JCV and required for the efficient replication of the viral genome. • This research highlights the first comprehensive proteomics analysis of Agno and identified 501 Agno targets in cells. • Data also revealed that Agno mostly targets the host proteins with “coiled-coil” domains. • Interaction of Agno with Rab11B, Importin and Exportin/Crm-1 was validated by protein-protein interaction studies." @default.
- W2981128682 created "2019-10-25" @default.
- W2981128682 creator A5002745087 @default.
- W2981128682 creator A5015843733 @default.
- W2981128682 creator A5046576978 @default.
- W2981128682 date "2020-01-01" @default.
- W2981128682 modified "2023-09-29" @default.
- W2981128682 title "A comprehensive proteomics analysis of JC virus Agnoprotein-interacting proteins: Agnoprotein primarily targets the host proteins with coiled-coil motifs" @default.
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- W2981128682 doi "https://doi.org/10.1016/j.virol.2019.10.005" @default.
- W2981128682 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6957716" @default.
- W2981128682 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31765920" @default.
- W2981128682 hasPublicationYear "2020" @default.