FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2981447246> ?p ?o ?g. }

• W2981447246 abstract "RNA interference (RNAi) is a biological process in which small RNAs regulate gene silencing at the transcriptional or posttranscriptional level. The trigger for gene silencing is double-stranded RNA generated from an endogenous genomic locus or a foreign source, such as a transgene or virus. In addition to regulating endogenous gene expression, RNAi provides the mechanistic basis for small RNA-mediated communication between plant hosts and interacting pathogenic microbes, known as cross-kingdom RNAi. Two core protein components, Argonaute (AGO) and Dicer (DCL), are central to the RNAi machinery of eukaryotes. Plants encode for several copies of AGO and DCL genes; in Arabidopsis thaliana, the AGO protein family contains 10 members, and the DCL family contains four. Little is known about the conservation and specific roles of these proteins in monocotyledonous plants, which account for the most important food staples. Here, we utilized in silico tools to investigate the structure and related functions of AGO and DCL proteins from the model grass Brachypodium distachyon. Based on the presence of characteristic domains, 16 BdAGO- and 6 BdDCL-predicted proteins were identified. Phylogenetic analysis showed that both protein families were expanded in Brachypodium as compared with Arabidopsis. For BdDCL proteins, both plant species contain a single copy of DCL1 and DCL4; however, Brachypodium contains two copies each of DCL2 and DCL3. Members of the BdAGO family were placed in all three functional clades of AGO proteins previously described in Arabidopsis. The greatest expansion occurred in the AtAGO1/5/10 clade, which contains nine BdAGOs (BdAGO5/6/7/9/10/11/12/15/16). The catalytic tetrad of the AGO P-element-induced wimpy testis domain (PIWI), which is required for endonuclease activity, is conserved in most BdAGOs, with the exception of BdAGO1, which lacks the last D/H residue. Three-dimensional modeling of BdAGO proteins using tertiary structure prediction software supported the phylogenetic classification. We also predicted a provisional interactome network for BdAGOs, their localization within the cell, and organ/tissue-specific expression. Exploring the specifics of RNAi machinery proteins in a model grass species can serve as a proxy for agronomically important cereals such as barley and wheat, where the development of RNAi-based plant protection strategies is of great interest." @default.
• W2981447246 created "2019-11-01" @default.
• W2981447246 creator A5008244232 @default.
• W2981447246 creator A5083851613 @default.
• W2981447246 creator A5090113829 @default.
• W2981447246 date "2019-10-22" @default.
• W2981447246 modified "2023-10-06" @default.
• W2981447246 title "Further Elucidation of the Argonaute and Dicer Protein Families in the Model Grass Species Brachypodium distachyon" @default.
• W2981447246 cites W1191273493 @default.
• W2981447246 cites W144423133 @default.
• W2981447246 cites W1521650410 @default.
• W2981447246 cites W1647075334 @default.
• W2981447246 cites W1864021403 @default.
• W2981447246 cites W1879776826 @default.
• W2981447246 cites W1963957860 @default.
• W2981447246 cites W1967276739 @default.
• W2981447246 cites W1967760135 @default.
• W2981447246 cites W1972171731 @default.
• W2981447246 cites W1977922844 @default.
• W2981447246 cites W1979938274 @default.
• W2981447246 cites W1982095104 @default.
• W2981447246 cites W1986191025 @default.
• W2981447246 cites W1986352294 @default.
• W2981447246 cites W1987029108 @default.
• W2981447246 cites W1987357838 @default.
• W2981447246 cites W1988801159 @default.
• W2981447246 cites W1993340457 @default.
• W2981447246 cites W1994873854 @default.
• W2981447246 cites W1996423252 @default.
• W2981447246 cites W2000709438 @default.
• W2981447246 cites W2002900187 @default.
• W2981447246 cites W2004798786 @default.
• W2981447246 cites W2008505479 @default.
• W2981447246 cites W2009206848 @default.
• W2981447246 cites W2013434849 @default.
• W2981447246 cites W2019739870 @default.
• W2981447246 cites W2021735982 @default.
• W2981447246 cites W2022687771 @default.
• W2981447246 cites W2026222098 @default.
• W2981447246 cites W2034394760 @default.
• W2981447246 cites W2035340311 @default.
• W2981447246 cites W2042975711 @default.
• W2981447246 cites W2045557677 @default.
• W2981447246 cites W2049392988 @default.
• W2981447246 cites W2049902088 @default.
• W2981447246 cites W2051210555 @default.
• W2981447246 cites W2055453791 @default.
• W2981447246 cites W2057106128 @default.
• W2981447246 cites W2057162139 @default.
• W2981447246 cites W2058085234 @default.
• W2981447246 cites W2059870368 @default.
• W2981447246 cites W2070062799 @default.
• W2981447246 cites W2083019620 @default.
• W2981447246 cites W2087123132 @default.
• W2981447246 cites W2088933990 @default.
• W2981447246 cites W2089427152 @default.
• W2981447246 cites W2098448352 @default.
• W2981447246 cites W2100932663 @default.
• W2981447246 cites W2102292691 @default.
• W2981447246 cites W2103546861 @default.
• W2981447246 cites W2104161044 @default.
• W2981447246 cites W2104189207 @default.
• W2981447246 cites W2110989021 @default.
• W2981447246 cites W2111092051 @default.
• W2981447246 cites W2113666425 @default.
• W2981447246 cites W2113807667 @default.
• W2981447246 cites W2116027790 @default.
• W2981447246 cites W2118980569 @default.
• W2981447246 cites W2119015197 @default.
• W2981447246 cites W2123846805 @default.
• W2981447246 cites W2126325893 @default.
• W2981447246 cites W2127322768 @default.
• W2981447246 cites W2130479394 @default.
• W2981447246 cites W2132926880 @default.
• W2981447246 cites W2136405950 @default.
• W2981447246 cites W2139115320 @default.
• W2981447246 cites W2139519158 @default.
• W2981447246 cites W2140733984 @default.
• W2981447246 cites W2141292707 @default.
• W2981447246 cites W2142338990 @default.
• W2981447246 cites W2142678478 @default.
• W2981447246 cites W2143826011 @default.
• W2981447246 cites W2151284271 @default.
• W2981447246 cites W2155973543 @default.
• W2981447246 cites W2157165549 @default.
• W2981447246 cites W2158178674 @default.
• W2981447246 cites W2159614853 @default.
• W2981447246 cites W2160730143 @default.
• W2981447246 cites W2162171603 @default.
• W2981447246 cites W2162313732 @default.
• W2981447246 cites W2163522597 @default.
• W2981447246 cites W2164392140 @default.
• W2981447246 cites W2169150541 @default.
• W2981447246 cites W2169379013 @default.
• W2981447246 cites W2172286727 @default.
• W2981447246 cites W2195136000 @default.
• W2981447246 cites W2263131847 @default.
• W2981447246 cites W2318448274 @default.
• W2981447246 cites W2336215964 @default.
• W2981447246 cites W2401135525 @default.

• next