Matches in SemOpenAlex for { <https://semopenalex.org/work/W2981799642> ?p ?o ?g. }
- W2981799642 endingPage "689" @default.
- W2981799642 startingPage "677" @default.
- W2981799642 abstract "A series of well-defined N-glycosylated IgG4-Fc variants were utilized to investigate the effect of glycan structure on their physicochemical properties (conformational stability and photostability) and interactions with an Fc γ receptor IIIA (FcγRIIIA). High mannose (HM, GlcNAc2Man(8+n) [n = 0-4]), Man5 (GlcNAc2Man5), GlcNAc1, and N297Q IgG4-Fc were prepared in good quality. The physical stability of these IgG4-Fc variants was examined with differential scanning calorimetry and intrinsic fluorescence spectroscopy. Photostability was assessed after photoirradiation between 295 and 340 nm (λ max = 305 nm), and HPLC-MS/MS analysis of specific products was performed. The size of glycans at Asn297 affects the yields of light-induced Tyr side-chain fragmentation products, where the yields decreased in the following order: N297Q > GlcNAc1 > Man5 > HM. These yields correlate with the thermal stability of the glycoforms. The HM and Man5 glycoforms display increased affinity for FcγRIIIA by at least 14.7-fold compared with GlcNAc1 IgG4-Fc. The affinities measured for the HM and Man5 IgG4-Fc (0.39-0.52 μM) are similar to those measured for fucosylated IgG1. Dependent on the mechanisms of action of IgG4 therapeutics, such glycoforms may need to be carefully monitored. The nonglycosylated N297Q IgG4-Fc did not present measurable affinity to FcγRIIIA." @default.
- W2981799642 created "2019-11-01" @default.
- W2981799642 creator A5016762726 @default.
- W2981799642 creator A5024842195 @default.
- W2981799642 creator A5029382209 @default.
- W2981799642 creator A5060120854 @default.
- W2981799642 creator A5090159873 @default.
- W2981799642 creator A5010275481 @default.
- W2981799642 date "2020-01-01" @default.
- W2981799642 modified "2023-10-03" @default.
- W2981799642 title "Effects of Glycan Structure on the Stability and Receptor Binding of an IgG4-Fc" @default.
- W2981799642 cites W1622362352 @default.
- W2981799642 cites W1831036856 @default.
- W2981799642 cites W1895658925 @default.
- W2981799642 cites W1965346959 @default.
- W2981799642 cites W1969824500 @default.
- W2981799642 cites W1976692354 @default.
- W2981799642 cites W1980042433 @default.
- W2981799642 cites W1983442876 @default.
- W2981799642 cites W1989407795 @default.
- W2981799642 cites W1991656907 @default.
- W2981799642 cites W2002121950 @default.
- W2981799642 cites W2005003298 @default.
- W2981799642 cites W2007100595 @default.
- W2981799642 cites W2034515273 @default.
- W2981799642 cites W2034766668 @default.
- W2981799642 cites W2037311267 @default.
- W2981799642 cites W2038048096 @default.
- W2981799642 cites W2038319340 @default.
- W2981799642 cites W2039021127 @default.
- W2981799642 cites W2048560240 @default.
- W2981799642 cites W2053459391 @default.
- W2981799642 cites W2072081310 @default.
- W2981799642 cites W2079579628 @default.
- W2981799642 cites W2083289596 @default.
- W2981799642 cites W2087168317 @default.
- W2981799642 cites W2092638671 @default.
- W2981799642 cites W2094052190 @default.
- W2981799642 cites W2098205015 @default.
- W2981799642 cites W2134159466 @default.
- W2981799642 cites W2135676217 @default.
- W2981799642 cites W2137354517 @default.
- W2981799642 cites W2153600773 @default.
- W2981799642 cites W2156471177 @default.
- W2981799642 cites W2164143412 @default.
- W2981799642 cites W2169656611 @default.
- W2981799642 cites W2175291039 @default.
- W2981799642 cites W2199771129 @default.
- W2981799642 cites W2222985666 @default.
- W2981799642 cites W2237518356 @default.
- W2981799642 cites W2257999935 @default.
- W2981799642 cites W2288947485 @default.
- W2981799642 cites W2318605753 @default.
- W2981799642 cites W2403376704 @default.
- W2981799642 cites W2478409189 @default.
- W2981799642 cites W2567453335 @default.
- W2981799642 cites W2596270747 @default.
- W2981799642 cites W2607161983 @default.
- W2981799642 cites W2653070086 @default.
- W2981799642 cites W2762727349 @default.
- W2981799642 cites W2783613440 @default.
- W2981799642 cites W2789654676 @default.
- W2981799642 cites W2790763558 @default.
- W2981799642 cites W2794416238 @default.
- W2981799642 cites W2800819859 @default.
- W2981799642 cites W2883979574 @default.
- W2981799642 cites W2890495176 @default.
- W2981799642 cites W2904051070 @default.
- W2981799642 cites W2904369050 @default.
- W2981799642 cites W2915483287 @default.
- W2981799642 doi "https://doi.org/10.1016/j.xphs.2019.10.036" @default.
- W2981799642 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31669606" @default.
- W2981799642 hasPublicationYear "2020" @default.
- W2981799642 type Work @default.
- W2981799642 sameAs 2981799642 @default.
- W2981799642 citedByCount "6" @default.
- W2981799642 countsByYear W29817996422020 @default.
- W2981799642 countsByYear W29817996422021 @default.
- W2981799642 countsByYear W29817996422023 @default.
- W2981799642 crossrefType "journal-article" @default.
- W2981799642 hasAuthorship W2981799642A5010275481 @default.
- W2981799642 hasAuthorship W2981799642A5016762726 @default.
- W2981799642 hasAuthorship W2981799642A5024842195 @default.
- W2981799642 hasAuthorship W2981799642A5029382209 @default.
- W2981799642 hasAuthorship W2981799642A5060120854 @default.
- W2981799642 hasAuthorship W2981799642A5090159873 @default.
- W2981799642 hasBestOaLocation W29817996421 @default.
- W2981799642 hasConcept C108625454 @default.
- W2981799642 hasConcept C121332964 @default.
- W2981799642 hasConcept C12554922 @default.
- W2981799642 hasConcept C159654299 @default.
- W2981799642 hasConcept C170493617 @default.
- W2981799642 hasConcept C178790620 @default.
- W2981799642 hasConcept C179998833 @default.
- W2981799642 hasConcept C185592680 @default.
- W2981799642 hasConcept C203014093 @default.
- W2981799642 hasConcept C206212055 @default.
- W2981799642 hasConcept C2780898057 @default.