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- W2981824650 abstract "The aim of this study is to evaluate the effect of ethylene glycol (EG), dimethylsulfoxide (DMSO) concentrations and compare the effect of several cryoprotectants on the viability and morphology of pig oocytes during vitrification and post-thawing. The percentage of post-thawing development 2-4 cells embryos reported with the use 10.0% (v/v) ethylene glycol (EG) + 5.0% (v/v) dimethylsulfoxide (DMSO) had a significantly high survival rate, but lower than the control. Trehalose extracellular cryoprotectant also has a higher rate of the viability after freezing than sucrose (73.38%; 62.44%; 31.45% and 13.19%) and (85.32%; 75.07%; 36.61% and 7.56%) (p < 0.05). Prolonged pre-incubation time after thawing adversely affects the rates of embryonic cleavage and development. In conclusion, vitrification is a simple technique and easy to perform but it needs some experience to prevent any oocyte loss during vitrification and thawing processing. The use of 10.0% (v/v) ethylene glycol (EG) + 5.0% (v/v) dimethylsulfoxide (DMSO) and trehalose in vitrification solution could improve the percentage of post-thawing viable and embryonic development." @default.
- W2981824650 created "2019-11-01" @default.
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- W2981824650 date "2019-01-01" @default.
- W2981824650 modified "2023-09-23" @default.
- W2981824650 title "Bảo quản lạnh tế bào trứng của heo ở giai đoạn trưởng thành bằng phương pháp thủy tinh hóa trong cryotech" @default.
- W2981824650 doi "https://doi.org/10.22144/ctu.jsi.2019.029" @default.
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