Matches in SemOpenAlex for { <https://semopenalex.org/work/W2981881567> ?p ?o ?g. }
- W2981881567 endingPage "1874.e7" @default.
- W2981881567 startingPage "1862" @default.
- W2981881567 abstract "Universal synthetic antibody (sAB)-based fiducial marks have been generated by customized phage display selections to facilitate the rapid structure determination of G protein-coupled receptor (GPCR) signaling complexes by single-particle cryo-electron microscopy (SP cryo-EM). sABs were generated to the two major G protein subclasses: trimeric Gi and Gs, as well as mini-Gs, and were tested to ensure binding in the context of their cognate GPCRs. Epitope binning revealed that multiple distinct epitopes exist for each G(αβγ) protein. Several Gβγ-specific sABs, cross-reactive between trimeric Gi and Gs, were identified suggesting they could be used across all subclasses in a plug and play fashion. sABs were also generated to a representative of another class of GPCR signaling partner, G protein receptor kinase 1 (GRK1) and evaluated further, supporting the generalizability of the approach. EM data suggested that the subclass-specific sABs provide effective single and dual fiducials for multiple GPCR signaling complexes." @default.
- W2981881567 created "2019-11-01" @default.
- W2981881567 creator A5000927614 @default.
- W2981881567 creator A5041615081 @default.
- W2981881567 creator A5045460299 @default.
- W2981881567 creator A5046500317 @default.
- W2981881567 creator A5050756960 @default.
- W2981881567 creator A5060277324 @default.
- W2981881567 creator A5060281780 @default.
- W2981881567 creator A5066381821 @default.
- W2981881567 creator A5068996594 @default.
- W2981881567 creator A5075896531 @default.
- W2981881567 creator A5082863229 @default.
- W2981881567 date "2019-12-01" @default.
- W2981881567 modified "2023-10-15" @default.
- W2981881567 title "Development of “Plug and Play” Fiducial Marks for Structural Studies of GPCR Signaling Complexes by Single-Particle Cryo-EM" @default.
- W2981881567 cites W1031578623 @default.
- W2981881567 cites W1560061125 @default.
- W2981881567 cites W1907908820 @default.
- W2981881567 cites W1969189143 @default.
- W2981881567 cites W1973260221 @default.
- W2981881567 cites W1978940629 @default.
- W2981881567 cites W1979900371 @default.
- W2981881567 cites W1982498753 @default.
- W2981881567 cites W1983335488 @default.
- W2981881567 cites W1989570792 @default.
- W2981881567 cites W2000106654 @default.
- W2981881567 cites W2024850327 @default.
- W2981881567 cites W2029667189 @default.
- W2981881567 cites W2035503835 @default.
- W2981881567 cites W2036931150 @default.
- W2981881567 cites W2039399096 @default.
- W2981881567 cites W2047567287 @default.
- W2981881567 cites W2051925784 @default.
- W2981881567 cites W2077936290 @default.
- W2981881567 cites W2079523532 @default.
- W2981881567 cites W2087663632 @default.
- W2981881567 cites W2093334168 @default.
- W2981881567 cites W2102663456 @default.
- W2981881567 cites W2113862155 @default.
- W2981881567 cites W2123520905 @default.
- W2981881567 cites W2129060396 @default.
- W2981881567 cites W2135451205 @default.
- W2981881567 cites W2147988069 @default.
- W2981881567 cites W2157269820 @default.
- W2981881567 cites W2159126254 @default.
- W2981881567 cites W2165775221 @default.
- W2981881567 cites W2167857702 @default.
- W2981881567 cites W2292743908 @default.
- W2981881567 cites W2499343687 @default.
- W2981881567 cites W2514617502 @default.
- W2981881567 cites W2516956609 @default.
- W2981881567 cites W2520075163 @default.
- W2981881567 cites W2524451022 @default.
- W2981881567 cites W2555870966 @default.
- W2981881567 cites W2593511418 @default.
- W2981881567 cites W2599786994 @default.
- W2981881567 cites W2605880739 @default.
- W2981881567 cites W2606915259 @default.
- W2981881567 cites W2607506319 @default.
- W2981881567 cites W2613102394 @default.
- W2981881567 cites W2615145336 @default.
- W2981881567 cites W2618233912 @default.
- W2981881567 cites W2728374022 @default.
- W2981881567 cites W2734817732 @default.
- W2981881567 cites W2777807685 @default.
- W2981881567 cites W2782382515 @default.
- W2981881567 cites W2783429882 @default.
- W2981881567 cites W2788934957 @default.
- W2981881567 cites W2793572103 @default.
- W2981881567 cites W2807850657 @default.
- W2981881567 cites W2808524678 @default.
- W2981881567 cites W2809171792 @default.
- W2981881567 cites W2810768392 @default.
- W2981881567 cites W2884888276 @default.
- W2981881567 cites W2889733245 @default.
- W2981881567 cites W2890544573 @default.
- W2981881567 cites W2902148700 @default.
- W2981881567 cites W2910852897 @default.
- W2981881567 cites W2939681516 @default.
- W2981881567 cites W2944441714 @default.
- W2981881567 cites W2944643389 @default.
- W2981881567 cites W2948859220 @default.
- W2981881567 cites W2953320394 @default.
- W2981881567 cites W2954773920 @default.
- W2981881567 cites W769965830 @default.
- W2981881567 doi "https://doi.org/10.1016/j.str.2019.10.004" @default.
- W2981881567 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7297049" @default.
- W2981881567 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31669042" @default.
- W2981881567 hasPublicationYear "2019" @default.
- W2981881567 type Work @default.
- W2981881567 sameAs 2981881567 @default.
- W2981881567 citedByCount "16" @default.
- W2981881567 countsByYear W29818815672020 @default.
- W2981881567 countsByYear W29818815672021 @default.
- W2981881567 countsByYear W29818815672022 @default.
- W2981881567 crossrefType "journal-article" @default.
- W2981881567 hasAuthorship W2981881567A5000927614 @default.