Matches in SemOpenAlex for { <https://semopenalex.org/work/W2982494927> ?p ?o ?g. }
- W2982494927 endingPage "1340" @default.
- W2982494927 startingPage "1340" @default.
- W2982494927 abstract ": Glycogen synthase kinase 3 (GSK3) slows myogenic differentiation and myoblast fusion partly by inhibiting the Wnt/β-catenin signaling pathway. Lithium, a common medication for bipolar disorder, inhibits GSK3 via Mg+ competition and increased Ser21 (GSK3α) or Ser9 (GSK3β) phosphorylation, leading to enhanced myoblast fusion and myogenic differentiation. However, previous studies demonstrating the effect of lithium on GSK3 have used concentrations up to 10 mM, which greatly exceeds concentrations measured in the serum of patients being treated for bipolar disorder (0.5–1.2 mM). Here, we determined whether a low-therapeutic (0.5 mM) dose of lithium could promote myoblast fusion and myogenic differentiation in C2C12 cells. C2C12 myotubes differentiated for three days in media containing 0.5 mM lithium chloride (LiCl) had significantly higher GSK3β (ser9) and GSK3α (ser21) phosphorylation compared with control myotubes differentiated in the same media without LiCl (+2–2.5 fold, p < 0.05), a result associated with an increase in total β-catenin. To further demonstrate that 0.5 mM LiCl inhibited GSK3 activity, we also developed a novel GSK3-specific activity assay. Using this enzyme-linked spectrophotometric assay, we showed that 0.5 mM LiCl-treated myotubes had significantly reduced GSK3 activity (−86%, p < 0.001). Correspondingly, 0.5 mM LiCl treated myotubes had a higher myoblast fusion index compared with control (p < 0.001) and significantly higher levels of markers of myogenesis (myogenin, +3-fold, p < 0.001) and myogenic differentiation (myosin heavy chain, +10-fold, p < 0.001). These results indicate that a low-therapeutic dose of LiCl is sufficient to promote myoblast fusion and myogenic differentiation in muscle cells, which has implications for the treatment of several myopathic conditions." @default.
- W2982494927 created "2019-11-08" @default.
- W2982494927 creator A5032382978 @default.
- W2982494927 creator A5034117156 @default.
- W2982494927 creator A5035809311 @default.
- W2982494927 creator A5037156246 @default.
- W2982494927 creator A5043927370 @default.
- W2982494927 creator A5045957326 @default.
- W2982494927 creator A5057102646 @default.
- W2982494927 creator A5063497866 @default.
- W2982494927 creator A5069548274 @default.
- W2982494927 creator A5082314311 @default.
- W2982494927 creator A5084361887 @default.
- W2982494927 creator A5087302904 @default.
- W2982494927 date "2019-10-29" @default.
- W2982494927 modified "2023-09-27" @default.
- W2982494927 title "A Low-Therapeutic Dose of Lithium Inhibits GSK3 and Enhances Myoblast Fusion in C2C12 Cells" @default.
- W2982494927 cites W1530473805 @default.
- W2982494927 cites W1979650304 @default.
- W2982494927 cites W1979993283 @default.
- W2982494927 cites W1980220309 @default.
- W2982494927 cites W1981766752 @default.
- W2982494927 cites W1986130046 @default.
- W2982494927 cites W1997546274 @default.
- W2982494927 cites W1999248384 @default.
- W2982494927 cites W2000448207 @default.
- W2982494927 cites W2004350402 @default.
- W2982494927 cites W2006059207 @default.
- W2982494927 cites W2017319459 @default.
- W2982494927 cites W2019041110 @default.
- W2982494927 cites W2035073601 @default.
- W2982494927 cites W2036787961 @default.
- W2982494927 cites W2037048755 @default.
- W2982494927 cites W2050539588 @default.
- W2982494927 cites W2051131116 @default.
- W2982494927 cites W2057868246 @default.
- W2982494927 cites W2059616389 @default.
- W2982494927 cites W2076937584 @default.
- W2982494927 cites W2077951355 @default.
- W2982494927 cites W2098985594 @default.
- W2982494927 cites W2100474560 @default.
- W2982494927 cites W2115629296 @default.
- W2982494927 cites W2128282944 @default.
- W2982494927 cites W2131379623 @default.
- W2982494927 cites W2136145695 @default.
- W2982494927 cites W2146432584 @default.
- W2982494927 cites W2164316354 @default.
- W2982494927 cites W2166636130 @default.
- W2982494927 cites W2200545821 @default.
- W2982494927 cites W2290466312 @default.
- W2982494927 cites W2322628263 @default.
- W2982494927 cites W2323313522 @default.
- W2982494927 cites W2337302529 @default.
- W2982494927 cites W2409540771 @default.
- W2982494927 cites W2520396547 @default.
- W2982494927 cites W2567335695 @default.
- W2982494927 cites W2582706725 @default.
- W2982494927 cites W2592190149 @default.
- W2982494927 cites W2593947784 @default.
- W2982494927 cites W2622415484 @default.
- W2982494927 cites W2752894194 @default.
- W2982494927 cites W2756991743 @default.
- W2982494927 cites W2768606470 @default.
- W2982494927 cites W2888137716 @default.
- W2982494927 cites W2890396273 @default.
- W2982494927 cites W2901524898 @default.
- W2982494927 cites W2937964159 @default.
- W2982494927 cites W2969379691 @default.
- W2982494927 cites W955230847 @default.
- W2982494927 cites W2416694469 @default.
- W2982494927 doi "https://doi.org/10.3390/cells8111340" @default.
- W2982494927 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6912290" @default.
- W2982494927 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31671858" @default.
- W2982494927 hasPublicationYear "2019" @default.
- W2982494927 type Work @default.
- W2982494927 sameAs 2982494927 @default.
- W2982494927 citedByCount "21" @default.
- W2982494927 countsByYear W29824949272020 @default.
- W2982494927 countsByYear W29824949272021 @default.
- W2982494927 countsByYear W29824949272022 @default.
- W2982494927 countsByYear W29824949272023 @default.
- W2982494927 crossrefType "journal-article" @default.
- W2982494927 hasAuthorship W2982494927A5032382978 @default.
- W2982494927 hasAuthorship W2982494927A5034117156 @default.
- W2982494927 hasAuthorship W2982494927A5035809311 @default.
- W2982494927 hasAuthorship W2982494927A5037156246 @default.
- W2982494927 hasAuthorship W2982494927A5043927370 @default.
- W2982494927 hasAuthorship W2982494927A5045957326 @default.
- W2982494927 hasAuthorship W2982494927A5057102646 @default.
- W2982494927 hasAuthorship W2982494927A5063497866 @default.
- W2982494927 hasAuthorship W2982494927A5069548274 @default.
- W2982494927 hasAuthorship W2982494927A5082314311 @default.
- W2982494927 hasAuthorship W2982494927A5084361887 @default.
- W2982494927 hasAuthorship W2982494927A5087302904 @default.
- W2982494927 hasBestOaLocation W29824949271 @default.
- W2982494927 hasConcept C104629339 @default.
- W2982494927 hasConcept C11960822 @default.
- W2982494927 hasConcept C126322002 @default.