Matches in SemOpenAlex for { <https://semopenalex.org/work/W2983088929> ?p ?o ?g. }
- W2983088929 endingPage "315" @default.
- W2983088929 startingPage "303" @default.
- W2983088929 abstract "Key points Increased insulin action is an important component of the health benefits of exercise, but its regulation is complex and not fully elucidated. Previous studies of insulin‐stimulated GLUT4 translocation to the skeletal muscle membrane found insufficient increases to explain the increases in glucose uptake. By determination of leg glucose uptake and interstitial muscle glucose concentration, insulin‐induced muscle membrane permeability to glucose was calculated 4 h after one‐legged knee‐extensor exercise during a submaximal euglycaemic–hyperinsulinaemic clamp. It was found that during submaximal insulin stimulation, muscle membrane permeability to glucose in humans increases twice as much in previously exercised vs . rested muscle and outstrips the supply of glucose, which then becomes limiting for glucose uptake. This methodology can now be employed to determine muscle membrane permeability to glucose in people with diabetes, who have reduced insulin action, and in principle can also be used to determine membrane permeability to other substrates or metabolites. Abstract Increased insulin action is an important component of the health benefits of exercise, but the regulation of insulin action in vivo is complex and not fully elucidated. Previously determined increases in skeletal muscle insulin‐stimulated GLUT4 translocation are inconsistent and mostly cannot explain the increases in insulin action in humans. Here we used leg glucose uptake (LGU) and interstitial muscle glucose concentration to calculate insulin‐induced muscle membrane permeability to glucose, a variable not previously possible to quantify in humans. Muscle membrane permeability to glucose, measured 4 h after one‐legged knee‐extensor exercise, increased ∼17‐fold during a submaximal euglycaemic–hyperinsulinaemic clamp in rested muscle (R) and ∼36‐fold in exercised muscle (EX). Femoral arterial infusion of N G ‐monomethyl l ‐arginine acetate or ATP decreased and increased, respectively, leg blood flow (LBF) in both legs but did not affect membrane glucose permeability. Decreasing LBF reduced interstitial glucose concentrations to ∼2 mM in the exercised but only to ∼3.5 mM in non‐exercised muscle and abrogated the augmented effect of insulin on LGU in the EX leg. Increasing LBF by ATP infusion increased LGU in both legs with uptake higher in the EX leg. We conclude that it is possible to measure functional muscle membrane permeability to glucose in humans and it increases twice as much in exercised vs . rested muscle during submaximal insulin stimulation. We also show that muscle perfusion is an important regulator of muscle glucose uptake when membrane permeability to glucose is high and we show that the capillary wall can be a significant barrier for glucose transport." @default.
- W2983088929 created "2019-11-22" @default.
- W2983088929 creator A5011413745 @default.
- W2983088929 creator A5027248926 @default.
- W2983088929 creator A5038581645 @default.
- W2983088929 creator A5039425655 @default.
- W2983088929 creator A5048291477 @default.
- W2983088929 creator A5063519527 @default.
- W2983088929 creator A5076018778 @default.
- W2983088929 creator A5087903094 @default.
- W2983088929 date "2020-01-01" @default.
- W2983088929 modified "2023-10-16" @default.
- W2983088929 title "Insulin‐induced membrane permeability to glucose in human muscles at rest and following exercise" @default.
- W2983088929 cites W1482754145 @default.
- W2983088929 cites W1565928898 @default.
- W2983088929 cites W1962620457 @default.
- W2983088929 cites W1972209552 @default.
- W2983088929 cites W1984231396 @default.
- W2983088929 cites W2000198788 @default.
- W2983088929 cites W2001078762 @default.
- W2983088929 cites W2003572473 @default.
- W2983088929 cites W2009181620 @default.
- W2983088929 cites W2010102296 @default.
- W2983088929 cites W2016489821 @default.
- W2983088929 cites W2017888726 @default.
- W2983088929 cites W2019691564 @default.
- W2983088929 cites W2032264548 @default.
- W2983088929 cites W2037546085 @default.
- W2983088929 cites W2041265416 @default.
- W2983088929 cites W2050211445 @default.
- W2983088929 cites W2051207867 @default.
- W2983088929 cites W2075171810 @default.
- W2983088929 cites W2075882099 @default.
- W2983088929 cites W2083264131 @default.
- W2983088929 cites W2085722628 @default.
- W2983088929 cites W2093190999 @default.
- W2983088929 cites W2094198536 @default.
- W2983088929 cites W2105912552 @default.
- W2983088929 cites W2108848770 @default.
- W2983088929 cites W2110464164 @default.
- W2983088929 cites W2113180848 @default.
- W2983088929 cites W2115569175 @default.
- W2983088929 cites W2116803850 @default.
- W2983088929 cites W2126847826 @default.
- W2983088929 cites W2127980806 @default.
- W2983088929 cites W2128258266 @default.
- W2983088929 cites W2128857489 @default.
- W2983088929 cites W2129849378 @default.
- W2983088929 cites W2141273174 @default.
- W2983088929 cites W2144019565 @default.
- W2983088929 cites W2146603936 @default.
- W2983088929 cites W2147361723 @default.
- W2983088929 cites W2149005146 @default.
- W2983088929 cites W2156595285 @default.
- W2983088929 cites W2157292761 @default.
- W2983088929 cites W2163600797 @default.
- W2983088929 cites W2167469946 @default.
- W2983088929 cites W2180033063 @default.
- W2983088929 cites W2218240027 @default.
- W2983088929 cites W2257942494 @default.
- W2983088929 cites W2285556835 @default.
- W2983088929 cites W2328294727 @default.
- W2983088929 cites W2337287667 @default.
- W2983088929 cites W2406512518 @default.
- W2983088929 cites W2407400602 @default.
- W2983088929 cites W2411064582 @default.
- W2983088929 cites W2413555955 @default.
- W2983088929 cites W248123225 @default.
- W2983088929 cites W2540741681 @default.
- W2983088929 cites W2596171931 @default.
- W2983088929 cites W2663870545 @default.
- W2983088929 cites W2888317499 @default.
- W2983088929 cites W2935563312 @default.
- W2983088929 cites W37478686 @default.
- W2983088929 cites W4248475202 @default.
- W2983088929 doi "https://doi.org/10.1113/jp278600" @default.
- W2983088929 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31696935" @default.
- W2983088929 hasPublicationYear "2020" @default.
- W2983088929 type Work @default.
- W2983088929 sameAs 2983088929 @default.
- W2983088929 citedByCount "33" @default.
- W2983088929 countsByYear W29830889292020 @default.
- W2983088929 countsByYear W29830889292021 @default.
- W2983088929 countsByYear W29830889292022 @default.
- W2983088929 countsByYear W29830889292023 @default.
- W2983088929 crossrefType "journal-article" @default.
- W2983088929 hasAuthorship W2983088929A5011413745 @default.
- W2983088929 hasAuthorship W2983088929A5027248926 @default.
- W2983088929 hasAuthorship W2983088929A5038581645 @default.
- W2983088929 hasAuthorship W2983088929A5039425655 @default.
- W2983088929 hasAuthorship W2983088929A5048291477 @default.
- W2983088929 hasAuthorship W2983088929A5063519527 @default.
- W2983088929 hasAuthorship W2983088929A5076018778 @default.
- W2983088929 hasAuthorship W2983088929A5087903094 @default.
- W2983088929 hasBestOaLocation W29830889291 @default.
- W2983088929 hasConcept C126322002 @default.
- W2983088929 hasConcept C134018914 @default.
- W2983088929 hasConcept C161573976 @default.