FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2983172327> ?p ?o ?g. }

• W2983172327 endingPage "1058" @default.
• W2983172327 startingPage "1058" @default.
• W2983172327 abstract "Viral isolation is desirable for many reasons, including development of diagnostic assays and reference materials, and for virology basic research. Zika virus (ZIKV) isolation from clinical samples is challenging, but isolates are known to infect various cell lines. Here, we evaluated suitability of Vero, C6/36 and JEG-3 as host cells, for direct isolation of ZIKV from human plasma. We also assessed the use of primary monocyte-derived macrophages (MDMs) culture to enhance ZIKV isolation from human plasma samples followed by virus expansion in Vero, C6/36 and JEG-3 cultures. Direct inoculation of cell lines with 42 ZIKV-RNA positive samples resulted in isolation rates of 9.52% (4/42) in Vero and C6/36, and of 7.14% (3/42) in JEG-3 cells. Inoculation of plasma in MDMs followed by supernatant testing by TaqMan RT-PCR, resulted in 33/42 (78.57%) ZIKV-RNA-positive supernatants, which expansion in cell lines increased isolation rates to 24.24% (8/33) in Vero and to 27.27% (9/33) in C6/36 and JEG-3 regardless of the presence of ZIKV-antibody. Isolates generated in JEG-3 cells were also produced in Vero and C6/36 with similar viral titers. These results suggest that efficiency of ZIKV isolation from human plasma can be enhanced when MDM culture is used before viral expansion in cell lines." @default.
• W2983172327 created "2019-11-22" @default.
• W2983172327 creator A5004048701 @default.
• W2983172327 creator A5016550815 @default.
• W2983172327 creator A5042596211 @default.
• W2983172327 creator A5058583424 @default.
• W2983172327 creator A5089304726 @default.
• W2983172327 date "2019-11-14" @default.
• W2983172327 modified "2023-09-27" @default.
• W2983172327 title "Use of Monocyte-Derived Macrophage Culture Increases Zika Virus Isolation Rate from Human Plasma" @default.
• W2983172327 cites W1545235721 @default.
• W2983172327 cites W1973619678 @default.
• W2983172327 cites W1975120587 @default.
• W2983172327 cites W2027816375 @default.
• W2983172327 cites W2053992388 @default.
• W2983172327 cites W2056690166 @default.
• W2983172327 cites W2068825716 @default.
• W2983172327 cites W2102144534 @default.
• W2983172327 cites W2114718288 @default.
• W2983172327 cites W2122349387 @default.
• W2983172327 cites W2131116699 @default.
• W2983172327 cites W2139124190 @default.
• W2983172327 cites W2281227512 @default.
• W2983172327 cites W2292786359 @default.
• W2983172327 cites W2295245778 @default.
• W2983172327 cites W2307185071 @default.
• W2983172327 cites W2341790184 @default.
• W2983172327 cites W2462844494 @default.
• W2983172327 cites W2508596867 @default.
• W2983172327 cites W2517528886 @default.
• W2983172327 cites W2537537963 @default.
• W2983172327 cites W2603997592 @default.
• W2983172327 cites W2742474627 @default.
• W2983172327 cites W2748285847 @default.
• W2983172327 cites W2759977828 @default.
• W2983172327 cites W2786367388 @default.
• W2983172327 cites W2791488016 @default.
• W2983172327 cites W2890984332 @default.
• W2983172327 cites W2900518613 @default.
• W2983172327 cites W2901798587 @default.
• W2983172327 cites W2949208852 @default.
• W2983172327 cites W2954941987 @default.
• W2983172327 cites W3011730446 @default.
• W2983172327 doi "https://doi.org/10.3390/v11111058" @default.
• W2983172327 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6893817" @default.
• W2983172327 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31739467" @default.
• W2983172327 hasPublicationYear "2019" @default.
• W2983172327 type Work @default.
• W2983172327 sameAs 2983172327 @default.
• W2983172327 citedByCount "3" @default.
• W2983172327 countsByYear W29831723272020 @default.
• W2983172327 countsByYear W29831723272021 @default.
• W2983172327 crossrefType "journal-article" @default.
• W2983172327 hasAuthorship W2983172327A5004048701 @default.
• W2983172327 hasAuthorship W2983172327A5016550815 @default.
• W2983172327 hasAuthorship W2983172327A5042596211 @default.
• W2983172327 hasAuthorship W2983172327A5058583424 @default.
• W2983172327 hasAuthorship W2983172327A5089304726 @default.
• W2983172327 hasBestOaLocation W29831723271 @default.
• W2983172327 hasConcept C159047783 @default.
• W2983172327 hasConcept C2522874641 @default.
• W2983172327 hasConcept C2775941552 @default.
• W2983172327 hasConcept C2777053367 @default.
• W2983172327 hasConcept C32611913 @default.
• W2983172327 hasConcept C54355233 @default.
• W2983172327 hasConcept C81885089 @default.
• W2983172327 hasConcept C86803240 @default.
• W2983172327 hasConcept C89138240 @default.
• W2983172327 hasConcept C89423630 @default.
• W2983172327 hasConceptScore W2983172327C159047783 @default.
• W2983172327 hasConceptScore W2983172327C2522874641 @default.
• W2983172327 hasConceptScore W2983172327C2775941552 @default.
• W2983172327 hasConceptScore W2983172327C2777053367 @default.
• W2983172327 hasConceptScore W2983172327C32611913 @default.
• W2983172327 hasConceptScore W2983172327C54355233 @default.
• W2983172327 hasConceptScore W2983172327C81885089 @default.
• W2983172327 hasConceptScore W2983172327C86803240 @default.
• W2983172327 hasConceptScore W2983172327C89138240 @default.
• W2983172327 hasConceptScore W2983172327C89423630 @default.
• W2983172327 hasIssue "11" @default.
• W2983172327 hasLocation W29831723271 @default.
• W2983172327 hasLocation W29831723272 @default.
• W2983172327 hasLocation W29831723273 @default.
• W2983172327 hasLocation W29831723274 @default.
• W2983172327 hasOpenAccess W2983172327 @default.
• W2983172327 hasPrimaryLocation W29831723271 @default.
• W2983172327 hasRelatedWork W1520753096 @default.
• W2983172327 hasRelatedWork W2156287975 @default.
• W2983172327 hasRelatedWork W2318808678 @default.
• W2983172327 hasRelatedWork W2359331237 @default.
• W2983172327 hasRelatedWork W2360621689 @default.
• W2983172327 hasRelatedWork W2367158143 @default.
• W2983172327 hasRelatedWork W2374080123 @default.
• W2983172327 hasRelatedWork W2392340475 @default.
• W2983172327 hasRelatedWork W2392487800 @default.
• W2983172327 hasRelatedWork W2765756010 @default.
• W2983172327 hasVolume "11" @default.
• W2983172327 isParatext "false" @default.

• next