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- W2983256454 abstract "hrf2 gene is a member of the harpin- encoding gene family of rice-pathogenic bacterium Xanthomonas oryzae pv. oryzicola. In our previous studies, we observed that harpinXooc could elicit hypersensitive cell death in non-host plants, induce disease and insect resistance in plants, and enhance plant growth. In this study, the rapeseed cultivar, Yangyou 4, was genetically engineered via Agrobac- terium-mediated transformation to express the hrf2 gene. Polymerase chain reaction (PCR) and southern blot analyses of T1 generation of transgenic rapeseed revealed stable integration and expression of the inserted gene hrf2. In addition, the resistance to Sclerotinia sclerotiorum was greatly enhanced. A comparison between agronomic characters of trans- genic and control lines displayed significant differ- ences in terms of plant height, stem width, number of pods per plant, number of seeds per pod, 1,000-seed weight, and seed yield per plant. Among lines with resistance to S. sclerotiorum ,T 11 had improved agronomic traits compared with controls with a 22.7% seed yield increase. These results suggest that the introduction of the hrf2 gene into rapeseed can be" @default.
- W2983256454 created "2019-11-22" @default.
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- W2983256454 date "2010-01-01" @default.
- W2983256454 modified "2023-09-27" @default.
- W2983256454 title "Assessment of inheritance pattern and agronomic performance of transgenic rapeseed having harpin Xooc -encoding hrf2 Gene" @default.
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