FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2983641569> ?p ?o ?g. }

• W2983641569 endingPage "1999" @default.
• W2983641569 startingPage "1985" @default.
• W2983641569 abstract "The HECT E3 ubiquitin ligase HUWE1 is required for a wide array of important functions in cell biology. Although HUWE1 is known to play a role in DNA damage signaling, the mechanism(s) that underlie this function remain elusive. HUWE1 regulates effectors of DNA replication and genotoxic stress tolerance. However, the loss of HUWE1 can also result in the accrual of significant endogenous DNA damage due to insufficient remediation of replication stress induced by an overabundance of key substrates. We discovered that HUWE1 depletion leads to a significant increase in levels of the single‐strand break effector kinase Chk1, independent of the DNA damage response, activation of apical DNA damage repair (DDR) signaling kinases (ATM and ATR), and the tumor suppressor p53. We also identified multiple lysine residues on Chk1 that are polyubiquitinated by HUWE1 in vitro , many of which are within the kinase domain. HUWE1 knockdown also markedly prolonged the protein half‐life of Chk1 in steady‐state conditions and resulted in greater stabilization of Chk1 protein than depletion of Cul4A, an E3 ubiquitin ligase previously described to control Chk1 abundance. Moreover, prolonged replication stress induced by hydroxyurea or camptothecin resulted in a reduction of Chk1 protein levels, which was rescued by HUWE1 knockdown. Our study indicates that HUWE1 plays a significant role in the regulation of the DDR signaling pathway by directly modulating the abundance of Chk1 protein." @default.
• W2983641569 created "2019-11-22" @default.
• W2983641569 creator A5012538768 @default.
• W2983641569 creator A5035751524 @default.
• W2983641569 creator A5061318946 @default.
• W2983641569 creator A5078110565 @default.
• W2983641569 date "2019-11-29" @default.
• W2983641569 modified "2023-10-15" @default.
• W2983641569 title "Direct regulation of Chk1 protein stability by E3 ubiquitin ligase HUWE1" @default.
• W2983641569 cites W1937647259 @default.
• W2983641569 cites W1969150779 @default.
• W2983641569 cites W1972166647 @default.
• W2983641569 cites W1983049322 @default.
• W2983641569 cites W1985716645 @default.
• W2983641569 cites W1991366673 @default.
• W2983641569 cites W1993897258 @default.
• W2983641569 cites W1999554125 @default.
• W2983641569 cites W2010691148 @default.
• W2983641569 cites W2013339424 @default.
• W2983641569 cites W2018214423 @default.
• W2983641569 cites W2020113131 @default.
• W2983641569 cites W2032030313 @default.
• W2983641569 cites W2032450998 @default.
• W2983641569 cites W2044553988 @default.
• W2983641569 cites W2051513922 @default.
• W2983641569 cites W2062547838 @default.
• W2983641569 cites W2076411120 @default.
• W2983641569 cites W2082018870 @default.
• W2983641569 cites W2082979850 @default.
• W2983641569 cites W2086098062 @default.
• W2983641569 cites W2093409463 @default.
• W2983641569 cites W2099219832 @default.
• W2983641569 cites W2105592350 @default.
• W2983641569 cites W2111403239 @default.
• W2983641569 cites W2112190173 @default.
• W2983641569 cites W2113684644 @default.
• W2983641569 cites W2113950661 @default.
• W2983641569 cites W2114794865 @default.
• W2983641569 cites W2116675584 @default.
• W2983641569 cites W2117273743 @default.
• W2983641569 cites W2120793206 @default.
• W2983641569 cites W2126917090 @default.
• W2983641569 cites W2129014947 @default.
• W2983641569 cites W2130200459 @default.
• W2983641569 cites W2137310763 @default.
• W2983641569 cites W2145197647 @default.
• W2983641569 cites W2145345398 @default.
• W2983641569 cites W2152441728 @default.
• W2983641569 cites W2154141161 @default.
• W2983641569 cites W2157918066 @default.
• W2983641569 cites W2159027654 @default.
• W2983641569 cites W2166805606 @default.
• W2983641569 cites W2167900035 @default.
• W2983641569 cites W2325375737 @default.
• W2983641569 cites W2346739653 @default.
• W2983641569 cites W2414840988 @default.
• W2983641569 cites W2553087578 @default.
• W2983641569 cites W2556028929 @default.
• W2983641569 cites W2586246044 @default.
• W2983641569 cites W2745836093 @default.
• W2983641569 cites W2756668630 @default.
• W2983641569 cites W2763372195 @default.
• W2983641569 cites W2788428532 @default.
• W2983641569 doi "https://doi.org/10.1111/febs.15132" @default.
• W2983641569 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7226928" @default.
• W2983641569 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31713291" @default.
• W2983641569 hasPublicationYear "2019" @default.
• W2983641569 type Work @default.
• W2983641569 sameAs 2983641569 @default.
• W2983641569 citedByCount "30" @default.
• W2983641569 countsByYear W29836415692020 @default.
• W2983641569 countsByYear W29836415692021 @default.
• W2983641569 countsByYear W29836415692022 @default.
• W2983641569 countsByYear W29836415692023 @default.
• W2983641569 crossrefType "journal-article" @default.
• W2983641569 hasAuthorship W2983641569A5012538768 @default.
• W2983641569 hasAuthorship W2983641569A5035751524 @default.
• W2983641569 hasAuthorship W2983641569A5061318946 @default.
• W2983641569 hasAuthorship W2983641569A5078110565 @default.
• W2983641569 hasBestOaLocation W29836415692 @default.
• W2983641569 hasConcept C104317684 @default.
• W2983641569 hasConcept C105696609 @default.
• W2983641569 hasConcept C134459356 @default.
• W2983641569 hasConcept C134935766 @default.
• W2983641569 hasConcept C143425029 @default.
• W2983641569 hasConcept C1491633281 @default.
• W2983641569 hasConcept C173396325 @default.
• W2983641569 hasConcept C175114707 @default.
• W2983641569 hasConcept C25602115 @default.
• W2983641569 hasConcept C2779682216 @default.
• W2983641569 hasConcept C29537977 @default.
• W2983641569 hasConcept C50001416 @default.
• W2983641569 hasConcept C51785407 @default.
• W2983641569 hasConcept C54355233 @default.
• W2983641569 hasConcept C552990157 @default.
• W2983641569 hasConcept C55493867 @default.
• W2983641569 hasConcept C73573662 @default.
• W2983641569 hasConcept C81885089 @default.

• next