FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2983708532> ?p ?o ?g. }

• W2983708532 abstract "Abstract Human mesenchymal stromal cells (MSCs) can replicate as undifferentiated cells in vitro and are capable of differentiating into multiple mesenchymal tissues. In the bone marrow, MSCs are thought to contribute to nurturing stroma involved in maintaining and modulating hematopoiesis. Toll-like receptors (TLRs) function as receptors for different conserved pathogen associated products as well as certain host derived molecules. TLRs are expressed in several hematopoietic and non-hematopoietic cells, and their activation plays a key role in innate and adaptive immune responses to infectious agents, as well as in the development of pathologic conditions like autoimmune disease. In inflammatory conditions like sepsis, cytokines like G-CSF are elevated in serum, CD34+ cells increase in circulation, and bone marrow myelopoiesis is enhanced. We hypothesized that MSCs express TLRs and are capable to respond to TLR agonists by changing their cytokine expression pattern in order to more efficiently support hematopoiesis according to respective needs. In vitro cultured bone marrow MSCs were analyzed for mRNA expression of TLRs. TLR 1, 3, 4, 5, 6, and 9 expression, but not TLR 2, 7, 8, and 10 expression was detectable. Compared to human conventional and plasmacytoid blood dendritic cells, MSCs expressed TLR-3 and TLR-4 at levels up to 2 log higher than did conventional DCs, while TLR 9 expression was low. Upon in vitro stimulation with TLR-4 agonists, mRNA transcripts of flt3-ligand and thrombopoietin increased, and MSCs secreted high amounts of G-CSF, GM-CSF, and M-CSF, whereas stimulation with TLR-9 agonists did not lead to changes in cytokine levels measured in supernatants. TLR-4 stimulated MSC conditioned media enhanced myeloid colony formation from human CD34+ cells about 2.5× fold compared to non-stimulated MSC conditioned media. Furthermore, in co-culture assays of MSCs with CD34+ cells, TLR-4 stimulated MSCs were capable to retain 8 fold more CD34+ cells in divisions 0–3 and maintained overall 2 fold higher numbers of CD34+ cells. In secondary colony formation assays, CD34+ cells from TLR-4 stimulated MSC co-cultures produced about 2.5 fold more myeloid colonies, with some being CFU-GEMM. Moreover, hematopoietic cells taken from TLR-4 stimulated MSC co-cultures were able to engraft conditioned newborn Rag 2−/− gc−/− mice and differentiated into B, T, and myeloid cells, while CD34+ cells from co-cultures of non-stimulated MSCs did not engraft. These findings thus suggest a regulatory mechanism how, e.g. in gram-negative infection, inflammatory signals are translated via stromal cells into sustained early hematopoiesis and myeloid differentiation to efficiently meet the requests of an ongoing innate immune response." @default.
• W2983708532 created "2019-11-22" @default.
• W2983708532 creator A5002232174 @default.
• W2983708532 creator A5008611632 @default.
• W2983708532 creator A5063546246 @default.
• W2983708532 creator A5091359051 @default.
• W2983708532 date "2008-11-16" @default.
• W2983708532 modified "2023-09-28" @default.
• W2983708532 title "TLR4-Agonist Stimulated Human Multipotent Mesenchymal Stromal Cells Enhance Both Hematopoietic Progenitors and Myeloid Differentiation" @default.
• W2983708532 doi "https://doi.org/10.1182/blood.v112.11.1366.1366" @default.
• W2983708532 hasPublicationYear "2008" @default.
• W2983708532 type Work @default.
• W2983708532 sameAs 2983708532 @default.
• W2983708532 citedByCount "0" @default.
• W2983708532 crossrefType "journal-article" @default.
• W2983708532 hasAuthorship W2983708532A5002232174 @default.
• W2983708532 hasAuthorship W2983708532A5008611632 @default.
• W2983708532 hasAuthorship W2983708532A5063546246 @default.
• W2983708532 hasAuthorship W2983708532A5091359051 @default.
• W2983708532 hasConcept C10205521 @default.
• W2983708532 hasConcept C109159458 @default.
• W2983708532 hasConcept C136449434 @default.
• W2983708532 hasConcept C16930146 @default.
• W2983708532 hasConcept C198826908 @default.
• W2983708532 hasConcept C203014093 @default.
• W2983708532 hasConcept C2776709828 @default.
• W2983708532 hasConcept C2779282312 @default.
• W2983708532 hasConcept C2780007613 @default.
• W2983708532 hasConcept C28328180 @default.
• W2983708532 hasConcept C502942594 @default.
• W2983708532 hasConcept C86803240 @default.
• W2983708532 hasConcept C8891405 @default.
• W2983708532 hasConcept C95444343 @default.
• W2983708532 hasConcept C95862172 @default.
• W2983708532 hasConceptScore W2983708532C10205521 @default.
• W2983708532 hasConceptScore W2983708532C109159458 @default.
• W2983708532 hasConceptScore W2983708532C136449434 @default.
• W2983708532 hasConceptScore W2983708532C16930146 @default.
• W2983708532 hasConceptScore W2983708532C198826908 @default.
• W2983708532 hasConceptScore W2983708532C203014093 @default.
• W2983708532 hasConceptScore W2983708532C2776709828 @default.
• W2983708532 hasConceptScore W2983708532C2779282312 @default.
• W2983708532 hasConceptScore W2983708532C2780007613 @default.
• W2983708532 hasConceptScore W2983708532C28328180 @default.
• W2983708532 hasConceptScore W2983708532C502942594 @default.
• W2983708532 hasConceptScore W2983708532C86803240 @default.
• W2983708532 hasConceptScore W2983708532C8891405 @default.
• W2983708532 hasConceptScore W2983708532C95444343 @default.
• W2983708532 hasConceptScore W2983708532C95862172 @default.
• W2983708532 hasLocation W29837085321 @default.
• W2983708532 hasOpenAccess W2983708532 @default.
• W2983708532 hasPrimaryLocation W29837085321 @default.
• W2983708532 hasRelatedWork W1485247791 @default.
• W2983708532 hasRelatedWork W1758327849 @default.
• W2983708532 hasRelatedWork W1933612924 @default.
• W2983708532 hasRelatedWork W1974916967 @default.
• W2983708532 hasRelatedWork W1977758974 @default.
• W2983708532 hasRelatedWork W1997797226 @default.
• W2983708532 hasRelatedWork W2002508526 @default.
• W2983708532 hasRelatedWork W2049317002 @default.
• W2983708532 hasRelatedWork W2064330449 @default.
• W2983708532 hasRelatedWork W2079678573 @default.
• W2983708532 hasRelatedWork W2124122909 @default.
• W2983708532 hasRelatedWork W2137229950 @default.
• W2983708532 hasRelatedWork W2153931057 @default.
• W2983708532 hasRelatedWork W2311721899 @default.
• W2983708532 hasRelatedWork W2467617886 @default.
• W2983708532 hasRelatedWork W2582123114 @default.
• W2983708532 hasRelatedWork W2720276201 @default.
• W2983708532 hasRelatedWork W2948225892 @default.
• W2983708532 hasRelatedWork W2980177177 @default.
• W2983708532 hasRelatedWork W3086565185 @default.
• W2983708532 isParatext "false" @default.
• W2983708532 isRetracted "false" @default.
• W2983708532 magId "2983708532" @default.
• W2983708532 workType "article" @default.