Matches in SemOpenAlex for { <https://semopenalex.org/work/W2984098685> ?p ?o ?g. }
- W2984098685 abstract "Summary Fibroblast Growth Factor 2 (FGF2) is a tumor cell survival factor that is exported from cells by an unconventional secretory pathway. This process is based on direct translocation of FGF2 across the plasma membrane. FGF2 membrane translocation depends on PI(4,5)P 2 -induced formation of membrane-inserted FGF2 oligomers followed by extracellular trapping of FGF2 at the outer leaflet mediated by cell surface heparan sulfate proteoglycans. Beyond the well-characterized core mechanism of FGF2 membrane translocation, the Na,K-ATPase has been proposed to play a so far unknown role in unconventional secretion of FGF2. Here, we define a direct physical interaction of FGF2 with a subdomain of the cytoplasmic part of the α1 subunit of the Na,K-ATPase. Employing NMR spectroscopy and molecular dynamics simulations, we identified two lysine residues on the molecular surface of FGF2 that are shown to be essential for its interaction with α1. In intact cells, the corresponding lysine-to-glutamate variants of FGF2 were characterized by inefficient secretion and reduced recruitment to the inner plasma membrane leaflet as shown by single molecule TIRF microscopy. Our findings suggest that α1 acts upstream of PI(4,5)P 2 facilitating efficient membrane translocation of FGF2 to the cell surface of tumor cells." @default.
- W2984098685 created "2019-11-22" @default.
- W2984098685 creator A5019049417 @default.
- W2984098685 creator A5022636758 @default.
- W2984098685 creator A5033245106 @default.
- W2984098685 creator A5042605431 @default.
- W2984098685 creator A5051080742 @default.
- W2984098685 creator A5054910477 @default.
- W2984098685 creator A5055755986 @default.
- W2984098685 creator A5059604181 @default.
- W2984098685 creator A5063280401 @default.
- W2984098685 creator A5065802674 @default.
- W2984098685 creator A5082983574 @default.
- W2984098685 creator A5085049301 @default.
- W2984098685 date "2019-11-01" @default.
- W2984098685 modified "2023-09-26" @default.
- W2984098685 title "The α1 subunit of the Na,K-ATPase acts upstream of PI(4,5)P2 facilitating unconventional secretion of Fibroblast Growth Factor 2 from tumor cells" @default.
- W2984098685 cites W1031578623 @default.
- W2984098685 cites W1795771003 @default.
- W2984098685 cites W1965928113 @default.
- W2984098685 cites W1968465516 @default.
- W2984098685 cites W1978084442 @default.
- W2984098685 cites W1986857435 @default.
- W2984098685 cites W1997062000 @default.
- W2984098685 cites W1997642309 @default.
- W2984098685 cites W2000443388 @default.
- W2984098685 cites W2000491758 @default.
- W2984098685 cites W2010544239 @default.
- W2984098685 cites W2016266703 @default.
- W2984098685 cites W2016417523 @default.
- W2984098685 cites W2016618099 @default.
- W2984098685 cites W2025116006 @default.
- W2984098685 cites W2027239974 @default.
- W2984098685 cites W2029392153 @default.
- W2984098685 cites W2029870588 @default.
- W2984098685 cites W2036054611 @default.
- W2984098685 cites W2044655036 @default.
- W2984098685 cites W2055733223 @default.
- W2984098685 cites W2058917763 @default.
- W2984098685 cites W2059307851 @default.
- W2984098685 cites W2059496396 @default.
- W2984098685 cites W2065635384 @default.
- W2984098685 cites W2067174909 @default.
- W2984098685 cites W2071739012 @default.
- W2984098685 cites W2081693079 @default.
- W2984098685 cites W2082031266 @default.
- W2984098685 cites W2094104389 @default.
- W2984098685 cites W2103063095 @default.
- W2984098685 cites W2111222016 @default.
- W2984098685 cites W2111387452 @default.
- W2984098685 cites W2119743134 @default.
- W2984098685 cites W2138757496 @default.
- W2984098685 cites W2142048790 @default.
- W2984098685 cites W2150351373 @default.
- W2984098685 cites W2163952331 @default.
- W2984098685 cites W2165623503 @default.
- W2984098685 cites W2165779834 @default.
- W2984098685 cites W2166317516 @default.
- W2984098685 cites W2167279371 @default.
- W2984098685 cites W2169707043 @default.
- W2984098685 cites W2170734232 @default.
- W2984098685 cites W2206954826 @default.
- W2984098685 cites W2269721101 @default.
- W2984098685 cites W2398601466 @default.
- W2984098685 cites W2460557882 @default.
- W2984098685 cites W2529052661 @default.
- W2984098685 cites W2555870966 @default.
- W2984098685 cites W2566427422 @default.
- W2984098685 cites W2606700140 @default.
- W2984098685 cites W2624924252 @default.
- W2984098685 cites W2737451970 @default.
- W2984098685 cites W2739239933 @default.
- W2984098685 cites W2768102094 @default.
- W2984098685 cites W2789444099 @default.
- W2984098685 cites W2800982196 @default.
- W2984098685 cites W2803992338 @default.
- W2984098685 cites W2887547606 @default.
- W2984098685 cites W2901340183 @default.
- W2984098685 cites W2901809796 @default.
- W2984098685 doi "https://doi.org/10.1101/827691" @default.
- W2984098685 hasPublicationYear "2019" @default.
- W2984098685 type Work @default.
- W2984098685 sameAs 2984098685 @default.
- W2984098685 citedByCount "0" @default.
- W2984098685 crossrefType "posted-content" @default.
- W2984098685 hasAuthorship W2984098685A5019049417 @default.
- W2984098685 hasAuthorship W2984098685A5022636758 @default.
- W2984098685 hasAuthorship W2984098685A5033245106 @default.
- W2984098685 hasAuthorship W2984098685A5042605431 @default.
- W2984098685 hasAuthorship W2984098685A5051080742 @default.
- W2984098685 hasAuthorship W2984098685A5054910477 @default.
- W2984098685 hasAuthorship W2984098685A5055755986 @default.
- W2984098685 hasAuthorship W2984098685A5059604181 @default.
- W2984098685 hasAuthorship W2984098685A5063280401 @default.
- W2984098685 hasAuthorship W2984098685A5065802674 @default.
- W2984098685 hasAuthorship W2984098685A5082983574 @default.
- W2984098685 hasAuthorship W2984098685A5085049301 @default.
- W2984098685 hasBestOaLocation W29840986851 @default.
- W2984098685 hasConcept C104292427 @default.
- W2984098685 hasConcept C104317684 @default.