FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2984741474> ?p ?o ?g. }

• W2984741474 endingPage "140319" @default.
• W2984741474 startingPage "140319" @default.
• W2984741474 abstract "The β-N-acetylhexosaminidase from Penicillium oxalicum (PoHex; EC 3.2.1.52) is a fungal glycosidase with an outstandingly high GalNAcase/GlcNAcase activity ratio. It has a remarkable synthetic capability and can process carbohydrates functionalized at various positions. However, the production in the native fungal host is lengthy, unselective and purification from the fungal medium is complicated and low yielding. We present here a novel production method of this enzyme in the eukaryotic host of Pichia pastoris, followed by elegant one-step purification to homogeneity. The resulting recombinant enzyme has improved biochemical and catalytic properties compared to the fungal wild type. Its good production yield (11 mg/400 mL cultivation medium) greatly expands the scope of synthetic applications. We further demonstrate the synthetic utility and broad acceptor specificity of recombinant PoHex in the glycosylation of a series of challenging acceptors with varying structural architectures, namely secondary and tertiary hydroxyl, aldoxime and a poly-hydroxylated compound." @default.
• W2984741474 created "2019-11-22" @default.
• W2984741474 creator A5010483782 @default.
• W2984741474 creator A5012601206 @default.
• W2984741474 creator A5052679373 @default.
• W2984741474 creator A5053169497 @default.
• W2984741474 creator A5074163299 @default.
• W2984741474 creator A5078632136 @default.
• W2984741474 date "2020-02-01" @default.
• W2984741474 modified "2023-10-17" @default.
• W2984741474 title "A novel enzymatic tool for transferring GalNAc moiety onto challenging acceptors" @default.
• W2984741474 cites W1519313422 @default.
• W2984741474 cites W1963810884 @default.
• W2984741474 cites W1983433391 @default.
• W2984741474 cites W2001081735 @default.
• W2984741474 cites W2030318875 @default.
• W2984741474 cites W2035397549 @default.
• W2984741474 cites W2044765444 @default.
• W2984741474 cites W2068332707 @default.
• W2984741474 cites W2076713265 @default.
• W2984741474 cites W2097559365 @default.
• W2984741474 cites W2110350794 @default.
• W2984741474 cites W2111963823 @default.
• W2984741474 cites W2162618268 @default.
• W2984741474 cites W2773022167 @default.
• W2984741474 cites W2790193543 @default.
• W2984741474 cites W2907626614 @default.
• W2984741474 cites W2913968715 @default.
• W2984741474 cites W2914088703 @default.
• W2984741474 cites W2919413130 @default.
• W2984741474 cites W2951292774 @default.
• W2984741474 cites W2967448555 @default.
• W2984741474 cites W4293247451 @default.
• W2984741474 doi "https://doi.org/10.1016/j.bbapap.2019.140319" @default.
• W2984741474 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31740417" @default.
• W2984741474 hasPublicationYear "2020" @default.
• W2984741474 type Work @default.
• W2984741474 sameAs 2984741474 @default.
• W2984741474 citedByCount "11" @default.
• W2984741474 countsByYear W29847414742020 @default.
• W2984741474 countsByYear W29847414742021 @default.
• W2984741474 countsByYear W29847414742022 @default.
• W2984741474 countsByYear W29847414742023 @default.
• W2984741474 crossrefType "journal-article" @default.
• W2984741474 hasAuthorship W2984741474A5010483782 @default.
• W2984741474 hasAuthorship W2984741474A5012601206 @default.
• W2984741474 hasAuthorship W2984741474A5052679373 @default.
• W2984741474 hasAuthorship W2984741474A5053169497 @default.
• W2984741474 hasAuthorship W2984741474A5074163299 @default.
• W2984741474 hasAuthorship W2984741474A5078632136 @default.
• W2984741474 hasConcept C104317684 @default.
• W2984741474 hasConcept C121332964 @default.
• W2984741474 hasConcept C134121241 @default.
• W2984741474 hasConcept C147816474 @default.
• W2984741474 hasConcept C181199279 @default.
• W2984741474 hasConcept C185592680 @default.
• W2984741474 hasConcept C191897082 @default.
• W2984741474 hasConcept C192562407 @default.
• W2984741474 hasConcept C196032511 @default.
• W2984741474 hasConcept C21951064 @default.
• W2984741474 hasConcept C26873012 @default.
• W2984741474 hasConcept C2776568683 @default.
• W2984741474 hasConcept C2777313579 @default.
• W2984741474 hasConcept C2779892579 @default.
• W2984741474 hasConcept C2781372317 @default.
• W2984741474 hasConcept C40767141 @default.
• W2984741474 hasConcept C55493867 @default.
• W2984741474 hasConcept C71240020 @default.
• W2984741474 hasConceptScore W2984741474C104317684 @default.
• W2984741474 hasConceptScore W2984741474C121332964 @default.
• W2984741474 hasConceptScore W2984741474C134121241 @default.
• W2984741474 hasConceptScore W2984741474C147816474 @default.
• W2984741474 hasConceptScore W2984741474C181199279 @default.
• W2984741474 hasConceptScore W2984741474C185592680 @default.
• W2984741474 hasConceptScore W2984741474C191897082 @default.
• W2984741474 hasConceptScore W2984741474C192562407 @default.
• W2984741474 hasConceptScore W2984741474C196032511 @default.
• W2984741474 hasConceptScore W2984741474C21951064 @default.
• W2984741474 hasConceptScore W2984741474C26873012 @default.
• W2984741474 hasConceptScore W2984741474C2776568683 @default.
• W2984741474 hasConceptScore W2984741474C2777313579 @default.
• W2984741474 hasConceptScore W2984741474C2779892579 @default.
• W2984741474 hasConceptScore W2984741474C2781372317 @default.
• W2984741474 hasConceptScore W2984741474C40767141 @default.
• W2984741474 hasConceptScore W2984741474C55493867 @default.
• W2984741474 hasConceptScore W2984741474C71240020 @default.
• W2984741474 hasFunder F4320321005 @default.
• W2984741474 hasIssue "2" @default.
• W2984741474 hasLocation W29847414741 @default.
• W2984741474 hasLocation W29847414742 @default.
• W2984741474 hasOpenAccess W2984741474 @default.
• W2984741474 hasPrimaryLocation W29847414741 @default.
• W2984741474 hasRelatedWork W1974455305 @default.
• W2984741474 hasRelatedWork W1997701291 @default.
• W2984741474 hasRelatedWork W2029341292 @default.
• W2984741474 hasRelatedWork W2063804016 @default.
• W2984741474 hasRelatedWork W2102116595 @default.
• W2984741474 hasRelatedWork W2622571117 @default.

• next