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- W2985057763 abstract "Decades of research have unraveled the complex functioning of neurons in the central nervous system. Our knowledge of the second main player of the brain – the non-excitable glial cells – clearly lags behind that of neurons. Pioneering work in the 1990s provided evidence that star-shaped glial cells – astrocytes – sense and modulate neuronal activity by intracellular Ca2+ signals. However, the precise roles of astrocytic Ca2+ signaling in brain physiology and pathophysiology are still highly controversial, largely due to technical limitations of previous Ca2+ imaging tools. With recent innovations in laser microscopy and engineering of molecular probes, the field of glioscience is undergoing a revolution. This chapter describes the application of multiphoton microscopy and genetically encoded fluorescent Ca2+ indicators to reveal astrocytic Ca2+ signals in acute brain slices and in vivo, both in anesthetized and in awake behaving animals." @default.
- W2985057763 created "2019-11-22" @default.
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- W2985057763 date "2019-01-01" @default.
- W2985057763 modified "2023-10-17" @default.
- W2985057763 title "Multiphoton Ca2+ Imaging of Astrocytes with Genetically Encoded Indicators Delivered by a Viral Approach" @default.
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- W2985057763 doi "https://doi.org/10.1007/978-1-4939-9702-2_11" @default.
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