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- W2985200704 abstract "Abstract Background Lentiviral vectors (LVs) allowing efficient establishment of stable transgene overexpression mammalian and human cell lines are invaluable tools for genetic research. Currently, although LV transductions are broadly adopted, they are often limited due to their low titers for efficient transduction. Results Here, we described a set of optimized, efficient techniques, which could produce sufficiently high LV titers, and, provide efficient transduction of cells. According to these optimizations, most of the mammalian and human cells, both primary cells and cell lines, could be transduced successfully with high levels of transgene stable expression, including both constitutive and induced expressions. Conclusions Our data demonstrated the highly usefulness of our optimized methods. Therefore, this study provided an efficient method for most of LV transduction experiments in vitro." @default.
- W2985200704 created "2019-11-22" @default.
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- W2985200704 date "2018-01-01" @default.
- W2985200704 modified "2023-09-26" @default.
- W2985200704 title "Efficient Lentiviral Transduction of Different Human and Mouse Cells" @default.
- W2985200704 hasPublicationYear "2018" @default.
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