Matches in SemOpenAlex for { <https://semopenalex.org/work/W2985266502> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W2985266502 abstract "Abstract Skeletal muscle retains the ability to regenerate throughout life, but this decreases significantly with aging. The present study investigates whether aging-associated loss of muscle hypoxia signaling limits regenerative potential. Utilizing young (3 months) and old (22-24 months) mice, skeletal muscle from old mice exhibited a 40% decline in the cross-sectional area (CSA) of newly regenerating fibers following cryoinjury at day 10 (p < 0.01) post-injury as compared to young. Focused PCR array demonstrated a greater than 3-fold decline in expression of the majority of hypoxia signaling genes. In particular, aryl hydrocarbon receptor nuclear translocator (ARNT), which is required for downstream hypoxia signaling and the transcription of hypoxia response genes, is 5-fold lower for both gene expression (p < 0.01) and protein levels (p < 0.01) in old versus young mice. To determine the effects of ARNT on muscle regeneration, we utilized a genetically modified mouse which results in an 80% decrease in ARNT gene expression following activation, specifically in skeletal muscle. Compared to littermate controls, mice with a muscle specific knockdown of ARNT (mKO ARNT) exhibit a 30% decline in regenerating fiber sizes at day 10 (p < 0.01) following cryoinjury, without any loss of regenerative potential in FACS isolated satellite cells ex vivo. Administration of a pharmacologic hypoxia activator, ML228, induced a 30% increase in regenerating fiber CSA in both old mice and mKO ARNT mice (p < 0.01) as compared to treatment with vehicle control. These data suggest hypoxia signaling declines with aging in skeletal muscle and activation of hypoxia signaling may promote regeneration." @default.
- W2985266502 created "2019-11-22" @default.
- W2985266502 creator A5001772518 @default.
- W2985266502 creator A5015735613 @default.
- W2985266502 creator A5024908381 @default.
- W2985266502 creator A5029695887 @default.
- W2985266502 creator A5030798882 @default.
- W2985266502 creator A5066205491 @default.
- W2985266502 creator A5066320106 @default.
- W2985266502 creator A5091846063 @default.
- W2985266502 date "2019-11-01" @default.
- W2985266502 modified "2023-09-23" @default.
- W2985266502 title "RESTORATION OF HYPOXIA SIGNALING IMPROVES AGING-ASSOCIATED LOSS OF SKELETAL MUSCLE REGENERATIVE POTENTIAL" @default.
- W2985266502 doi "https://doi.org/10.1093/geroni/igz038.2679" @default.
- W2985266502 hasPublicationYear "2019" @default.
- W2985266502 type Work @default.
- W2985266502 sameAs 2985266502 @default.
- W2985266502 citedByCount "0" @default.
- W2985266502 crossrefType "journal-article" @default.
- W2985266502 hasAuthorship W2985266502A5001772518 @default.
- W2985266502 hasAuthorship W2985266502A5015735613 @default.
- W2985266502 hasAuthorship W2985266502A5024908381 @default.
- W2985266502 hasAuthorship W2985266502A5029695887 @default.
- W2985266502 hasAuthorship W2985266502A5030798882 @default.
- W2985266502 hasAuthorship W2985266502A5066205491 @default.
- W2985266502 hasAuthorship W2985266502A5066320106 @default.
- W2985266502 hasAuthorship W2985266502A5091846063 @default.
- W2985266502 hasBestOaLocation W29852665021 @default.
- W2985266502 hasConcept C105702510 @default.
- W2985266502 hasConcept C178790620 @default.
- W2985266502 hasConcept C185592680 @default.
- W2985266502 hasConcept C2779959927 @default.
- W2985266502 hasConcept C540031477 @default.
- W2985266502 hasConcept C7836513 @default.
- W2985266502 hasConcept C86803240 @default.
- W2985266502 hasConcept C95444343 @default.
- W2985266502 hasConceptScore W2985266502C105702510 @default.
- W2985266502 hasConceptScore W2985266502C178790620 @default.
- W2985266502 hasConceptScore W2985266502C185592680 @default.
- W2985266502 hasConceptScore W2985266502C2779959927 @default.
- W2985266502 hasConceptScore W2985266502C540031477 @default.
- W2985266502 hasConceptScore W2985266502C7836513 @default.
- W2985266502 hasConceptScore W2985266502C86803240 @default.
- W2985266502 hasConceptScore W2985266502C95444343 @default.
- W2985266502 hasLocation W29852665021 @default.
- W2985266502 hasLocation W29852665022 @default.
- W2985266502 hasOpenAccess W2985266502 @default.
- W2985266502 hasPrimaryLocation W29852665021 @default.
- W2985266502 hasRelatedWork W1151243895 @default.
- W2985266502 hasRelatedWork W153138336 @default.
- W2985266502 hasRelatedWork W1991116678 @default.
- W2985266502 hasRelatedWork W2134588930 @default.
- W2985266502 hasRelatedWork W2154291688 @default.
- W2985266502 hasRelatedWork W2193057007 @default.
- W2985266502 hasRelatedWork W2286206290 @default.
- W2985266502 hasRelatedWork W2529112623 @default.
- W2985266502 hasRelatedWork W2551014352 @default.
- W2985266502 hasRelatedWork W2588438495 @default.
- W2985266502 hasRelatedWork W2790521860 @default.
- W2985266502 hasRelatedWork W2915337426 @default.
- W2985266502 hasRelatedWork W3012081751 @default.
- W2985266502 hasRelatedWork W3035616020 @default.
- W2985266502 hasRelatedWork W3089441285 @default.
- W2985266502 hasRelatedWork W3117130821 @default.
- W2985266502 hasRelatedWork W3149783855 @default.
- W2985266502 hasRelatedWork W3170382043 @default.
- W2985266502 hasRelatedWork W3198573900 @default.
- W2985266502 hasRelatedWork W3040261286 @default.
- W2985266502 isParatext "false" @default.
- W2985266502 isRetracted "false" @default.
- W2985266502 magId "2985266502" @default.
- W2985266502 workType "article" @default.