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- W2985388581 abstract "Breast cancer shows a high affinity toward bone, causing bone-related complications, leading to a poor clinical prognosis. The Wnt/β-catenin signaling pathway has been well-documented for the bone regenerative process; however, the regulation of the Wnt/β-catenin pathway in breast cancer bone metastasis is poorly explored. Here, we report that the Wnt/β-catenin signaling pathway has a significant effect on osteogenesis during breast cancer bone metastasis. In this study, we have created a 3D in vitro breast cancer bone metastatic microenvironment using nanoclay-based scaffolds along with osteogenically differentiated human mesenchymal stem cells (MSCs) and human breast cancer cells (MCF-7 and MDA-MB-231). The results showed upregulation in expressions of Wnt-related factors (Wnt-5a, β-catenin, AXIN2, and LRP5) in sequential cultures of MSCs with MCF-7 as compared to sequential cultures of MSCs with MDA-MB-231. Sequential cultures of MSCs with MCF-7 also showed higher β-catenin expression on the protein levels than sequential cultures of MSCs with MDA-MB-231. Stimulation of Wnt/β-catenin signaling in sequential cultures of MSCs with MCF-7 by ET-1 resulted in increased bone formation, whereas inactivation of Wnt/β-catenin signaling by DKK-1 displayed a significant decrease in bone formation, mimicking bone lesions in breast cancer patients. These data collectively demonstrate that Wnt/β-catenin signaling governs osteogenesis within the tumor-harboring bone microenvironment, leading to bone metastasis. The nanoclay scaffold provides a unique testbed approach for analysis of the pathways of cancer metastasis." @default.
- W2985388581 created "2019-11-22" @default.
- W2985388581 creator A5019289420 @default.
- W2985388581 creator A5033427217 @default.
- W2985388581 creator A5050874035 @default.
- W2985388581 creator A5071721520 @default.
- W2985388581 date "2019-11-01" @default.
- W2985388581 modified "2023-10-14" @default.
- W2985388581 title "Wnt/β-Catenin Signaling Pathway Regulates Osteogenesis for Breast Cancer Bone Metastasis: Experiments in an <i>In Vitro</i> Nanoclay Scaffold Cancer Testbed" @default.
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- W2985388581 doi "https://doi.org/10.1021/acsbiomaterials.9b00923" @default.
- W2985388581 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33463270" @default.
- W2985388581 hasPublicationYear "2019" @default.