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• W2986041804 endingPage "115309" @default.
• W2986041804 startingPage "115309" @default.
• W2986041804 abstract "This study investigated the chemical and genetic mechanisms of anaerobic fermentation of waste activated sludge (WAS) with CaO2 addition at ambient temperature. The microbial community structures, key microorganisms, functional profiles and related carbohydrate-active enzymes were further revealed according to metagenomic sequencing combining with 16S rRNA gene amplicon sequencing. Results showed that the prolonged period of alkaline condition generated from CaO2 contributed significantly to the continuous destruction of WAS, and the oxidative environment caused by CaO2 further enhanced flocs dissolution. This synergistic effect also significantly changed the microbial community. Oxidation contributed more than the alkaline condition to the decline of microbial diversity, while the effect of alkaline condition was greater than that of oxidation in the change of microbial community structure. The key enhanced genes associated with fatty acid biosynthesis pathways with CaO2 addition were highlighted. Three kinds of high-abundance acetyl-CoA carboxylase genes and eleven kinds of synthetase, hydrolase, lyase and oxidoreductase genes promoted by CaO2 were distributed throughout each branch of fatty acid biosynthesis pathway (ko00061). Moreover, carbohydrate binding modules (CBMs) and glycoside hydrolases (GHs) were the top two carbohydrate-active enzymes (CAZymes) improved by CaO2 addition. CaO2 can also effectively promote the function of lysozyme and the metabolism of several monosaccharides. This work provides a deep insight into the advantage of CaO2 in promoting sludge solubilization and acidification at the genetic levels, thus expanding the application of CaO2 in sludge treatment and resource recovery." @default.
• W2986041804 created "2019-11-22" @default.
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• W2986041804 date "2020-03-01" @default.
• W2986041804 modified "2023-10-12" @default.
• W2986041804 title "Metagenomic characterization of the enhanced performance of anaerobic fermentation of waste activated sludge with CaO2 addition at ambient temperature: Fatty acid biosynthesis metabolic pathway and CAZymes" @default.
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• W2986041804 doi "https://doi.org/10.1016/j.watres.2019.115309" @default.
• W2986041804 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31812814" @default.
• W2986041804 hasPublicationYear "2020" @default.
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