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- W2986093791 abstract "Abstract Deep-UV (DUV) excitation fluorescence microscopy has potential to provide rapid diagnosis with simple technique comparing to conventional histopathology based on hematoxylin and eosin (H&E) staining. We established a fluorescent staining protocol for DUV excitation fluorescence imaging that has enabled clear discrimination of nucleoplasm, nucleolus, and cytoplasm. Fluorescence images of metastasis-positive/-negative lymph nodes of gastric cancer patients were used for patch-based training with a deep neural network (DNN) based on Inception-v3 architecture. The performance on small patches of the fluorescence images was comparable with that of H&E images. Gradient-weighted class activation mapping analysis revealed the areas where the trained model identified metastatic lesions in the images containing cancer cells. We extended the method to large-size image analysis enabling accurate detection of metastatic lesions. We discuss usefulness of DUV excitation fluorescence imaging with the aid of DNN analysis, which is promising for assisting pathologists in assessment of lymph node metastasis." @default.
- W2986093791 created "2019-11-22" @default.
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- W2986093791 date "2019-11-15" @default.
- W2986093791 modified "2023-10-09" @default.
- W2986093791 title "Deep-UV excitation fluorescence microscopy for detection of lymph node metastasis using deep neural network" @default.
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- W2986093791 doi "https://doi.org/10.1038/s41598-019-53405-w" @default.
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