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- W2986133695 abstract "Abstract Solid‐state NMR (ssNMR) spectroscopy has evolved into a powerful method to obtain structural information and to study the dynamics of proteins at atomic resolution and under physiological conditions. The method is especially well suited to investigate insoluble and noncrystalline proteins that cannot be investigated easily by X‐ray crystallography or solution NMR. To allow for detailed analysis of ssNMR data, the assignment of resonances to the protein atoms is essential. For this purpose, a set of three‐dimensional (3D) spectra needs to be acquired. Band‐selective homo‐nuclear cross‐polarization (BSH‐CP) is an effective method for magnetization transfer between carbonyl carbon (CO) and alpha carbon (CA) atoms, which is an important transfer step in multidimensional ssNMR experiments. This tutorial describes the detailed procedure for the chemical shift assignment of the backbone atoms of 13 C– 15 N‐labeled proteins by BSH‐CP‐based 13 C‐detected ssNMR experiments. A set of six 3D experiments is used for unambiguous assignment of the protein backbone as well as certain side‐chain resonances. The tutorial especially addresses scientists with little experience in the field of ssNMR and provides all the necessary information for protein assignment in an efficient, time‐saving approach." @default.
- W2986133695 created "2019-11-22" @default.
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- W2986133695 date "2019-12-17" @default.
- W2986133695 modified "2023-10-02" @default.
- W2986133695 title "Protein resonance assignment by BSH‐CP‐based 3D solid‐state NMR experiments: A practical guide" @default.
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- W2986133695 doi "https://doi.org/10.1002/mrc.4945" @default.
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