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- W2986405873 abstract "Abstract Background β-carotene (provitamin A) is an important target for biofortification of crops as a potential solution to the problem of vitamin A deficiency that is prevalent in developing countries. A previous report showed that dominant expression of splicing variants in the Orange ( Or ) gene causes β-carotene accumulation in cauliflower curd. In this study, we focused on a putative orthologue of the cauliflower or gene in rice, Osor , and attempt to accumulate β-carotene in rice callus by modification of the Osor gene via genome editing using CRISPR/Cas9. Findings CRISPR/Cas9 vectors for the Osor gene were constructed and transformed into rice calli. Some transformed calli showed orange color due to β-carotene hyper-accumulation. Molecular analyses suggest that orange-colored calli are due to an abundance of in-frame aberrant Osor transcripts, whereas out-of-frame mutations were not associated with orange color. Conclusions We demonstrate that directed gene modification of the Osor gene via CRISPR/Cas9-mediated genome editing results in β-carotene fortification in rice calli. To date, golden rice, which accumulates β-carotene in rice endosperm, has been developed by conventional transgenic approaches. Our results suggest an alternative approach to enhancing β-carotene accumulation in crops." @default.
- W2986405873 created "2019-11-22" @default.
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- W2986405873 date "2019-11-12" @default.
- W2986405873 modified "2023-10-11" @default.
- W2986405873 title "A novel approach to carotenoid accumulation in rice callus by mimicking the cauliflower Orange mutation via genome editing" @default.
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- W2986405873 doi "https://doi.org/10.1186/s12284-019-0345-3" @default.
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