FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2986543304> ?p ?o ?g. }

• W2986543304 endingPage "156" @default.
• W2986543304 startingPage "156" @default.
• W2986543304 abstract "Nacre is composed of aragonite platelets and organic material formed by molluscs as inner shell layer through biomineralization process, and mantle epithelial cells control nacre formation. A primary culture of granulated epithelial cells was established from mantle tissue of Pinctada margaritifera, through a process of continuous sub-culturing at 28°C in yeast supplemented sea water culture medium. Nuclear beads were placed in culture wells containing semi-solid agar medium and incubated in vitro with cultured granulated epithelial cells in order to evaluate the nacre secretion. On visual observation, a brown coloration was observed on the surface of the bead after 7-10 days. Evaluation of the surface of the nuclear beads by scanning electron microscopy (SEM) after 60 days of incubation revealed a good brick and mortar pattern, characteristic of nacreous layer formation. A lustrous hue was also seen to develop on bead surfaces after this stage. SEM images of a cross section of the nacre-coated bead showed a pattern of arrangement of aragonite tablets similar to that seen in cross sections of the nacre layer of shell of molluscs. The functional ability of cultured granulated epithelial cells was further confirmed by detecting gene expression of two matrix proteins, nacrein and amorphous calcium carbonate binding proteins (ACCBP), which play an important role in formation of the nacreous layer, in both cultured cells and in native mantle tissue. Amplification products for nacrein (480 bp) and ACCBP (500 bp) genes were obtained in both native mantle tissue and in vitro cultured mantle epithelial cells. There was good correlation between the expression patterns of the two genes in in vitro cultured cells and in native mantle tissue, signifying that cultured mantle epithelial cells retain their functional characteristics of biomineralization." @default.
• W2986543304 created "2019-11-22" @default.
• W2986543304 creator A5016831024 @default.
• W2986543304 creator A5034777771 @default.
• W2986543304 creator A5038904722 @default.
• W2986543304 creator A5056061060 @default.
• W2986543304 date "2019-11-07" @default.
• W2986543304 modified "2023-10-16" @default.
• W2986543304 title "Cell Culture and Gene Expression Studies in Relation to Biomineralization in the Black-lip Pearl Oyster, Pinctada margaritifera" @default.
• W2986543304 cites W1509697555 @default.
• W2986543304 cites W1518297837 @default.
• W2986543304 cites W2007766907 @default.
• W2986543304 cites W2012172826 @default.
• W2986543304 cites W2022665330 @default.
• W2986543304 cites W2037951645 @default.
• W2986543304 cites W2038172849 @default.
• W2986543304 cites W2039706217 @default.
• W2986543304 cites W2040070479 @default.
• W2986543304 cites W2058701958 @default.
• W2986543304 cites W2059479609 @default.
• W2986543304 cites W2069306478 @default.
• W2986543304 cites W2090544314 @default.
• W2986543304 cites W2090577776 @default.
• W2986543304 cites W2139334271 @default.
• W2986543304 cites W2146472525 @default.
• W2986543304 cites W2151742908 @default.
• W2986543304 cites W2156034142 @default.
• W2986543304 cites W2159545917 @default.
• W2986543304 cites W2160127680 @default.
• W2986543304 cites W2176916863 @default.
• W2986543304 cites W2270507894 @default.
• W2986543304 cites W2317486887 @default.
• W2986543304 cites W2327389452 @default.
• W2986543304 cites W2776883828 @default.
• W2986543304 cites W2808215552 @default.
• W2986543304 doi "https://doi.org/10.2112/si86-024.1" @default.
• W2986543304 hasPublicationYear "2019" @default.
• W2986543304 type Work @default.
• W2986543304 sameAs 2986543304 @default.
• W2986543304 citedByCount "1" @default.
• W2986543304 countsByYear W29865433042022 @default.
• W2986543304 crossrefType "journal-article" @default.
• W2986543304 hasAuthorship W2986543304A5016831024 @default.
• W2986543304 hasAuthorship W2986543304A5034777771 @default.
• W2986543304 hasAuthorship W2986543304A5038904722 @default.
• W2986543304 hasAuthorship W2986543304A5056061060 @default.
• W2986543304 hasBestOaLocation W29865433042 @default.
• W2986543304 hasConcept C105702510 @default.
• W2986543304 hasConcept C12554922 @default.
• W2986543304 hasConcept C138885662 @default.
• W2986543304 hasConcept C151730666 @default.
• W2986543304 hasConcept C153911025 @default.
• W2986543304 hasConcept C159985019 @default.
• W2986543304 hasConcept C183586093 @default.
• W2986543304 hasConcept C185592680 @default.
• W2986543304 hasConcept C192562407 @default.
• W2986543304 hasConcept C27206212 @default.
• W2986543304 hasConcept C2778039128 @default.
• W2986543304 hasConcept C2779251273 @default.
• W2986543304 hasConcept C2779866092 @default.
• W2986543304 hasConcept C2781390083 @default.
• W2986543304 hasConcept C3018112274 @default.
• W2986543304 hasConcept C67236022 @default.
• W2986543304 hasConcept C86803240 @default.
• W2986543304 hasConcept C95444343 @default.
• W2986543304 hasConceptScore W2986543304C105702510 @default.
• W2986543304 hasConceptScore W2986543304C12554922 @default.
• W2986543304 hasConceptScore W2986543304C138885662 @default.
• W2986543304 hasConceptScore W2986543304C151730666 @default.
• W2986543304 hasConceptScore W2986543304C153911025 @default.
• W2986543304 hasConceptScore W2986543304C159985019 @default.
• W2986543304 hasConceptScore W2986543304C183586093 @default.
• W2986543304 hasConceptScore W2986543304C185592680 @default.
• W2986543304 hasConceptScore W2986543304C192562407 @default.
• W2986543304 hasConceptScore W2986543304C27206212 @default.
• W2986543304 hasConceptScore W2986543304C2778039128 @default.
• W2986543304 hasConceptScore W2986543304C2779251273 @default.
• W2986543304 hasConceptScore W2986543304C2779866092 @default.
• W2986543304 hasConceptScore W2986543304C2781390083 @default.
• W2986543304 hasConceptScore W2986543304C3018112274 @default.
• W2986543304 hasConceptScore W2986543304C67236022 @default.
• W2986543304 hasConceptScore W2986543304C86803240 @default.
• W2986543304 hasConceptScore W2986543304C95444343 @default.
• W2986543304 hasIssue "sp1" @default.
• W2986543304 hasLocation W29865433041 @default.
• W2986543304 hasLocation W29865433042 @default.
• W2986543304 hasOpenAccess W2986543304 @default.
• W2986543304 hasPrimaryLocation W29865433041 @default.
• W2986543304 hasRelatedWork W1966720328 @default.
• W2986543304 hasRelatedWork W2034740400 @default.
• W2986543304 hasRelatedWork W2081955829 @default.
• W2986543304 hasRelatedWork W2225458733 @default.
• W2986543304 hasRelatedWork W2342049808 @default.
• W2986543304 hasRelatedWork W2511492014 @default.
• W2986543304 hasRelatedWork W2741370550 @default.
• W2986543304 hasRelatedWork W2986543304 @default.
• W2986543304 hasRelatedWork W4280490716 @default.
• W2986543304 hasRelatedWork W638678128 @default.

• next