Matches in SemOpenAlex for { <https://semopenalex.org/work/W2986689789> ?p ?o ?g. }
- W2986689789 abstract "Abstract Background Corynebacterium ammoniagenes is an important industrial organism that is widely used to produce nucleotides and the potential for industrial production of coenzyme A by C. ammoniagenes ATCC 6871 has been shown. However, the yield of coenzyme A needs to be improved, and the available constitutive promoters are rather limited in this strain. Results In this study, 20 putative DNA promoters derived from genes with high transcription levels and 6 promoters from molecular chaperone genes were identified. To evaluate the activity of each promoter, red fluorescence protein (RFP) was used as a reporter. We successfully isolated a range of promoters with different activity levels, and among these a fragment derived from the upstream sequence of the 50S ribosomal protein L21 (P rpl21 ) exhibited the strongest activity among the 26 identified promoters. Furthermore, type III pantothenate kinase from Pseudomonas putida ( Pp coaA) was overexpressed in C. ammoniagenes under the control of P rpl21 , CoA yield increased approximately 4.4 times. Conclusions This study provides a paradigm for rational isolation of promoters with different activities and their application in metabolic engineering. These promoters will enrich the available promoter toolkit for C. ammoniagenes and should be valuable in current platforms for metabolic engineering and synthetic biology for the optimization of pathways to extend the product spectrum or improve the productivity in C. ammoniagenes ATCC 6871 for industrial applications." @default.
- W2986689789 created "2019-11-22" @default.
- W2986689789 creator A5004635944 @default.
- W2986689789 creator A5007838570 @default.
- W2986689789 creator A5013228738 @default.
- W2986689789 creator A5083031482 @default.
- W2986689789 creator A5083768870 @default.
- W2986689789 creator A5090830175 @default.
- W2986689789 date "2019-11-12" @default.
- W2986689789 modified "2023-10-07" @default.
- W2986689789 title "Isolating promoters from Corynebacterium ammoniagenes ATCC 6871 and application in CoA synthesis" @default.
- W2986689789 cites W1602119380 @default.
- W2986689789 cites W1789221514 @default.
- W2986689789 cites W18423076 @default.
- W2986689789 cites W1933579256 @default.
- W2986689789 cites W1948751406 @default.
- W2986689789 cites W1970422609 @default.
- W2986689789 cites W1971439989 @default.
- W2986689789 cites W1980571020 @default.
- W2986689789 cites W2003426471 @default.
- W2986689789 cites W2008851453 @default.
- W2986689789 cites W2016667239 @default.
- W2986689789 cites W2018645639 @default.
- W2986689789 cites W2020509224 @default.
- W2986689789 cites W2053454885 @default.
- W2986689789 cites W2063151014 @default.
- W2986689789 cites W2064417765 @default.
- W2986689789 cites W2070388868 @default.
- W2986689789 cites W2073384372 @default.
- W2986689789 cites W2075793022 @default.
- W2986689789 cites W2080857264 @default.
- W2986689789 cites W2087873683 @default.
- W2986689789 cites W2093453531 @default.
- W2986689789 cites W2094919783 @default.
- W2986689789 cites W2106696982 @default.
- W2986689789 cites W2113296192 @default.
- W2986689789 cites W2114156535 @default.
- W2986689789 cites W2122346488 @default.
- W2986689789 cites W2131884256 @default.
- W2986689789 cites W2304123352 @default.
- W2986689789 cites W2324730783 @default.
- W2986689789 cites W2334779110 @default.
- W2986689789 cites W2345455213 @default.
- W2986689789 cites W2415342604 @default.
- W2986689789 cites W2510573454 @default.
- W2986689789 cites W2515234215 @default.
- W2986689789 cites W283587943 @default.
- W2986689789 cites W345774762 @default.
- W2986689789 cites W89496163 @default.
- W2986689789 doi "https://doi.org/10.1186/s12896-019-0568-9" @default.
- W2986689789 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6849255" @default.
- W2986689789 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31718625" @default.
- W2986689789 hasPublicationYear "2019" @default.
- W2986689789 type Work @default.
- W2986689789 sameAs 2986689789 @default.
- W2986689789 citedByCount "2" @default.
- W2986689789 countsByYear W29866897892022 @default.
- W2986689789 crossrefType "journal-article" @default.
- W2986689789 hasAuthorship W2986689789A5004635944 @default.
- W2986689789 hasAuthorship W2986689789A5007838570 @default.
- W2986689789 hasAuthorship W2986689789A5013228738 @default.
- W2986689789 hasAuthorship W2986689789A5083031482 @default.
- W2986689789 hasAuthorship W2986689789A5083768870 @default.
- W2986689789 hasAuthorship W2986689789A5090830175 @default.
- W2986689789 hasBestOaLocation W29866897891 @default.
- W2986689789 hasConcept C101762097 @default.
- W2986689789 hasConcept C104317684 @default.
- W2986689789 hasConcept C138885662 @default.
- W2986689789 hasConcept C150194340 @default.
- W2986689789 hasConcept C179926584 @default.
- W2986689789 hasConcept C191908910 @default.
- W2986689789 hasConcept C2776260651 @default.
- W2986689789 hasConcept C2780543182 @default.
- W2986689789 hasConcept C41895202 @default.
- W2986689789 hasConcept C54355233 @default.
- W2986689789 hasConcept C55493867 @default.
- W2986689789 hasConcept C6350086 @default.
- W2986689789 hasConcept C86803240 @default.
- W2986689789 hasConceptScore W2986689789C101762097 @default.
- W2986689789 hasConceptScore W2986689789C104317684 @default.
- W2986689789 hasConceptScore W2986689789C138885662 @default.
- W2986689789 hasConceptScore W2986689789C150194340 @default.
- W2986689789 hasConceptScore W2986689789C179926584 @default.
- W2986689789 hasConceptScore W2986689789C191908910 @default.
- W2986689789 hasConceptScore W2986689789C2776260651 @default.
- W2986689789 hasConceptScore W2986689789C2780543182 @default.
- W2986689789 hasConceptScore W2986689789C41895202 @default.
- W2986689789 hasConceptScore W2986689789C54355233 @default.
- W2986689789 hasConceptScore W2986689789C55493867 @default.
- W2986689789 hasConceptScore W2986689789C6350086 @default.
- W2986689789 hasConceptScore W2986689789C86803240 @default.
- W2986689789 hasFunder F4320321001 @default.
- W2986689789 hasIssue "1" @default.
- W2986689789 hasLocation W29866897891 @default.
- W2986689789 hasLocation W29866897892 @default.
- W2986689789 hasLocation W29866897893 @default.
- W2986689789 hasLocation W29866897894 @default.
- W2986689789 hasLocation W29866897895 @default.
- W2986689789 hasLocation W29866897896 @default.
- W2986689789 hasOpenAccess W2986689789 @default.