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- W2986805274 abstract "Objective: Cerebral ischemia is accompanied by damage and death of a significant number of neurons due to glutamate excitotoxicity with subsequent a global increase of cytosolic Ca2+ concentration ([Ca2+]i). This study aimed to investigate the neuroprotective action of BDNF overexpression in hippocampal neurons against injury under ischemia-like conditions (oxygen and glucose deprivation) and glutamate-induced excitotoxicity (GluTox).Methods: The overexpression of BDNF was reached by the transduction of cell cultures with the adeno-associated (AAV)-Syn-BDNF-EGFP virus construct. Neuroprotective effects were mediated by Ca2+-dependent BDNF release followed by activation of the neuroprotective signaling cascades and changes of the gene expression. Thus, BDNF overexpression modulates Ca2+ homeostasis in cells, preventing Ca2+ overload and initiation of apoptotic and necrotic processes.Results:Antiapoptotic effect of BDNF overexpression is mediated via activation of phosphoinositide-3-kinase (PI3K) pathway and changing the expression of PI3K, HIF-1, Src and an anti-inflammatory cytokine IL-10. On the contrary, the decrease of expression of proapoptotic proteins such as Jun, Mapk8, caspase-3 and an inflammatory cytokine IL-1β was observed. These changes of expression were accompanied by the decrease of quantity of IL-1β receptors and the level of TNFα in cells in control, as well as 24 h after OGD. Besides, BDNF overexpression changes the expression of GABA(B) receptors. Also, the expression of NMDA and AMPA receptor subunits was altered towards a change in the conductivity of the receptors for Ca2+.Conclusion: Thus, our results demonstrate that neuronal BDNF overexpression reveals complex neuroprotective effects on the neurons and astrocytes under OGD and GluTox via inhibition of Ca2+ responses and regulation of gene expression." @default.
- W2986805274 created "2019-11-22" @default.
- W2986805274 creator A5000874785 @default.
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- W2986805274 creator A5073133233 @default.
- W2986805274 date "2019-11-26" @default.
- W2986805274 modified "2023-10-06" @default.
- W2986805274 title "The selective BDNF overexpression in neurons protects neuroglial networks against OGD and glutamate-induced excitotoxicity" @default.
- W2986805274 cites W1515260227 @default.
- W2986805274 cites W1555679702 @default.
- W2986805274 cites W1689734502 @default.
- W2986805274 cites W1894943419 @default.
- W2986805274 cites W1936849452 @default.
- W2986805274 cites W1964849051 @default.
- W2986805274 cites W1966798058 @default.
- W2986805274 cites W1967316112 @default.
- W2986805274 cites W1972317764 @default.
- W2986805274 cites W1973631822 @default.
- W2986805274 cites W1974173911 @default.
- W2986805274 cites W1975823509 @default.
- W2986805274 cites W1976347128 @default.
- W2986805274 cites W1978215017 @default.
- W2986805274 cites W1979406476 @default.
- W2986805274 cites W1985114553 @default.
- W2986805274 cites W1995530486 @default.
- W2986805274 cites W1995733166 @default.
- W2986805274 cites W1996893328 @default.
- W2986805274 cites W1998913081 @default.
- W2986805274 cites W2002360249 @default.
- W2986805274 cites W2003352171 @default.
- W2986805274 cites W2006061461 @default.
- W2986805274 cites W2006206804 @default.
- W2986805274 cites W2008608010 @default.
- W2986805274 cites W2009711649 @default.
- W2986805274 cites W2014033528 @default.
- W2986805274 cites W2016843660 @default.
- W2986805274 cites W2017850724 @default.
- W2986805274 cites W2017940437 @default.
- W2986805274 cites W2022196959 @default.
- W2986805274 cites W2024126366 @default.
- W2986805274 cites W2026623398 @default.
- W2986805274 cites W2029565457 @default.
- W2986805274 cites W2035090622 @default.
- W2986805274 cites W2035891064 @default.
- W2986805274 cites W2035983112 @default.
- W2986805274 cites W2037383099 @default.
- W2986805274 cites W2039379548 @default.
- W2986805274 cites W2040806880 @default.
- W2986805274 cites W2045527578 @default.
- W2986805274 cites W2046051810 @default.
- W2986805274 cites W2051312958 @default.
- W2986805274 cites W2055583081 @default.
- W2986805274 cites W2055697901 @default.
- W2986805274 cites W2059507131 @default.
- W2986805274 cites W2060936720 @default.
- W2986805274 cites W2066386560 @default.
- W2986805274 cites W2069780662 @default.
- W2986805274 cites W2074824347 @default.
- W2986805274 cites W2075992165 @default.
- W2986805274 cites W2076411353 @default.
- W2986805274 cites W2077897416 @default.
- W2986805274 cites W2079472132 @default.
- W2986805274 cites W2080203024 @default.
- W2986805274 cites W2085423995 @default.
- W2986805274 cites W2088912287 @default.
- W2986805274 cites W2094544368 @default.
- W2986805274 cites W2098231907 @default.
- W2986805274 cites W2105012597 @default.
- W2986805274 cites W2107277218 @default.
- W2986805274 cites W2110696099 @default.
- W2986805274 cites W2118655588 @default.
- W2986805274 cites W2124806566 @default.
- W2986805274 cites W2148950534 @default.
- W2986805274 cites W2150473092 @default.
- W2986805274 cites W2150929583 @default.
- W2986805274 cites W2153068303 @default.
- W2986805274 cites W2154727263 @default.
- W2986805274 cites W2171403507 @default.
- W2986805274 cites W2281956262 @default.
- W2986805274 cites W2411439969 @default.
- W2986805274 cites W2570525591 @default.
- W2986805274 cites W2768128866 @default.
- W2986805274 cites W2773999101 @default.
- W2986805274 cites W2805506771 @default.
- W2986805274 cites W2805557712 @default.
- W2986805274 cites W2883712923 @default.
- W2986805274 cites W2885248803 @default.
- W2986805274 cites W2913118409 @default.
- W2986805274 cites W3000965011 @default.
- W2986805274 doi "https://doi.org/10.1080/00207454.2019.1691205" @default.
- W2986805274 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31694441" @default.
- W2986805274 hasPublicationYear "2019" @default.
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