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- W2986885921 abstract "Chimeric RNAs are the transcripts composed of exons from two separate genes or transcripts. Although the presence of these joined RNA molecules have mainly been documented in a variety of eukaryotes, we recently demonstrated that the Ll.LtrB group II intron, from the gram-positive bacterium Lactococcus lactis, can generate chimeric mRNAs through a novel intergenic trans-splicing pathway. Here we describe the detailed experimental procedures to detect group II intron-generated mRNA-mRNA chimeras from total RNA extracts using stringent reverse transcription conditions along with a reverse splicing-deficient group II intron as a negative control." @default.
- W2986885921 created "2019-11-22" @default.
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- W2986885921 date "2019-11-15" @default.
- W2986885921 modified "2023-10-14" @default.
- W2986885921 title "Detection of Group II Intron-Generated Chimeric mRNAs in Bacterial Cells" @default.
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- W2986885921 doi "https://doi.org/10.1007/978-1-4939-9904-0_7" @default.
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