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• W2987294373 abstract "Understanding gene regulation networks in multicellular organisms is crucial to decipher many complex physiological processes ranging from development to aging. One technique to characterize gene expression with tissue-specificity in whole organisms is single-molecule fluorescence in situ hybridization (smFISH). However, this protocol requires lengthy incubation times, and it is challenging to achieve multiplexed smFISH in a whole organism. Multiplexing techniques can yield transcriptome-level information, but they require sequential probing of different genes. The inefficient macromolecule exchange through diffusion-dominant transport across dense tissues is the major bottleneck. In this work, we address this challenge by developing a microfluidic/electrokinetic hybrid platform to enable multicycle smFISH in an intact model organism, Caenorhabditis elegans. We integrate an ion concentration polarization based ion pump with a microfluidic array to rapidly deliver and remove gene-specific probes and stripping reagents on demand in individual animals. Using our platform, we can achieve rapid smFISH, an order of magnitude faster than traditional smFISH protocols. We also demonstrate the capability to perform multicycle smFISH on the same individual samples, which is impossible to do off-chip. Our method hence provides a powerful tool to study individual-specific, spatially resolvable, and large-scale gene expression in whole organisms." @default.
• W2987294373 created "2019-11-22" @default.
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• W2987294373 date "2019-11-01" @default.
• W2987294373 modified "2023-10-15" @default.
• W2987294373 title "Rapid and multi-cycle smFISH enabled by microfluidic ion concentration polarization for <i>in-situ</i> profiling of tissue-specific gene expression in whole <i>C. elegans</i>" @default.
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• W2987294373 doi "https://doi.org/10.1063/1.5124827" @default.
• W2987294373 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6824911" @default.
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