FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2987431035> ?p ?o ?g. }

• W2987431035 endingPage "50" @default.
• W2987431035 startingPage "SCI" @default.
• W2987431035 abstract "Stretched out from end-to-end, the human genome -a sequence of 3 billion chemical letters inscribed in a molecule called DNA -is over 2 meters long. Famously, short stretches of DNA fold into a double helix, which wind around histone proteins to form the 10nm fiber. But what about longer pieces? Does the genome's fold influence function? How does the information contained in such an ultra-dense packing even remain accessible?In this talk, I describe our work developing 'Hi-C' (Lieberman-Aiden et al., Science, 2009; Aiden, Science, 2011) and more recently 'in-situHi-C' (Rao & Huntley et al., Cell, 2014), which use proximity ligation to transform pairs of physically adjacent DNA loci into chimeric DNA sequences. Sequencing a library of such chimeras makes it possible to create genome-wide maps of physical contacts between pairs of loci, revealing features ofgenome folding in 3D. Next, I will describe recent work using in situ Hi-C to construct haploid and diploid maps of nine cell types. The densest, in human lymphoblastoid cells, contains 4.9 billion contacts, achieving 1 kb resolution. We find that genomes are partitioned into contact domains (median length, 185 kb), which are associated with distinct patterns of histone marks and segregate into six subcompartments. We identify ∼10,000 loops. These loops frequently link promoters and enhancers, correlate with gene activation, and show conservation across cell types and species. Loop anchors typically occur at domain boundaries and bind the protein CTCF. The CTCF motifs at loop anchors occur predominantly (>90%) in a convergent orientation, with the asymmetric motifs facing one another. Next, I will discuss the biophysical mechanism that underlies chromatin looping. Specifically, our data is consistent with the formation of loops by extrusion (Sanborn & Rao et al., PNAS, 2015). In fact, in many cases, the local structure of Hi-C maps may be predicted in silicobased on patterns of CTCF binding and an extrusion-based model. Finally, I will show that by modifying CTCF motifs using CRISPR, we can reliably add, move, and delete loops and domains. Thus, it possible not only to read the genome's 3D architecture, but also to write it. Disclosures No relevant conflicts of interest to declare." @default.
• W2987431035 created "2019-11-22" @default.
• W2987431035 creator A5004683234 @default.
• W2987431035 date "2019-11-13" @default.
• W2987431035 modified "2023-09-30" @default.
• W2987431035 title "Three-D Codes in the Human Genome" @default.
• W2987431035 doi "https://doi.org/10.1182/blood-2019-121474" @default.
• W2987431035 hasPublicationYear "2019" @default.
• W2987431035 type Work @default.
• W2987431035 sameAs 2987431035 @default.
• W2987431035 citedByCount "0" @default.
• W2987431035 crossrefType "journal-article" @default.
• W2987431035 hasAuthorship W2987431035A5004683234 @default.
• W2987431035 hasConcept C104317684 @default.
• W2987431035 hasConcept C111936080 @default.
• W2987431035 hasConcept C141231307 @default.
• W2987431035 hasConcept C197077220 @default.
• W2987431035 hasConcept C2778760011 @default.
• W2987431035 hasConcept C54355233 @default.
• W2987431035 hasConcept C552990157 @default.
• W2987431035 hasConcept C64927066 @default.
• W2987431035 hasConcept C70721500 @default.
• W2987431035 hasConcept C86339819 @default.
• W2987431035 hasConcept C86803240 @default.
• W2987431035 hasConceptScore W2987431035C104317684 @default.
• W2987431035 hasConceptScore W2987431035C111936080 @default.
• W2987431035 hasConceptScore W2987431035C141231307 @default.
• W2987431035 hasConceptScore W2987431035C197077220 @default.
• W2987431035 hasConceptScore W2987431035C2778760011 @default.
• W2987431035 hasConceptScore W2987431035C54355233 @default.
• W2987431035 hasConceptScore W2987431035C552990157 @default.
• W2987431035 hasConceptScore W2987431035C64927066 @default.
• W2987431035 hasConceptScore W2987431035C70721500 @default.
• W2987431035 hasConceptScore W2987431035C86339819 @default.
• W2987431035 hasConceptScore W2987431035C86803240 @default.
• W2987431035 hasIssue "Supplement_1" @default.
• W2987431035 hasLocation W29874310351 @default.
• W2987431035 hasOpenAccess W2987431035 @default.
• W2987431035 hasPrimaryLocation W29874310351 @default.
• W2987431035 hasRelatedWork W1442603624 @default.
• W2987431035 hasRelatedWork W1603543036 @default.
• W2987431035 hasRelatedWork W1995265584 @default.
• W2987431035 hasRelatedWork W2017378473 @default.
• W2987431035 hasRelatedWork W2042930277 @default.
• W2987431035 hasRelatedWork W2157750191 @default.
• W2987431035 hasRelatedWork W2974705896 @default.
• W2987431035 hasRelatedWork W2975451038 @default.
• W2987431035 hasRelatedWork W2094069166 @default.
• W2987431035 hasRelatedWork W2187207551 @default.
• W2987431035 hasVolume "134" @default.
• W2987431035 isParatext "false" @default.
• W2987431035 isRetracted "false" @default.
• W2987431035 magId "2987431035" @default.
• W2987431035 workType "article" @default.