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- W2987551876 abstract "The fact that the BETA radiation from /sup 3/H atoms incorporated into cellular DNA is absorbed largely in the nucleus, while more of that from /sup 3/H incorporated into RNA is absorbed in the cytoplasm than in the nucleus, provides a means of determiring the relative importance of nuclear and cytoplasmic damage in radioinduced mammalian cell death. Synchronously dividing populntions of Chinese hamster fibroblasts were pulse-labeled with various doses of either /sup 3/H-thymidine or /sup 3/H-uridine. The labeled cells were plated, and survival curves obtained. Autoradiographic silver grain counts and liquid scirtillation counting were used to measure the nuclear BETA radiation doses. It was found that the slope of the /sup 3/H-uridine survival curve was somewhat greater than that of the /sup 3/H-thymidine curve. Since the BETA radiation dose to the cytoplasm was larger than that to the nucleus, however, it is concluded that the contribution of cytoplasmic damage to radioinduced reproductive death of mammalian ceils is very small. (auth)" @default.
- W2987551876 created "2019-11-22" @default.
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- W2987551876 date "1963-09-01" @default.
- W2987551876 modified "2023-09-28" @default.
- W2987551876 title "A COMPARISON OF MAMMALIAN CELL-KILLING BY INCORPORATED $sup 3$H-THYMIDINE AND $sup 3$H-URIDINE" @default.
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