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- W2987996966 abstract "Objective To investigate the effects of TIMP-1 gene expression inhibition on the cell growth in human embryonic lung fibroblast MRC-5 induced by tumor necrosis factor-α (TNF-α). Methods MRC-5 cells were divided into blank group, TGF-β1 group, TGF-β1 group transfected with negative control small interfering RNA, and TGF-β1 group transfected with TIMP-1 small interfering RNA (siTIMP-1). The cell viability was measured by MTT assay. The cell cycle of MRC-5 was analyzed by MTT assay and flow cytometry. The content of TNF-α in the supernatant was detected by ELISA, and the protein expression of TIMP-1, α-SMA, collagen 1 and β-catenin were determined by Western blot analysis. Results The expression level of TIMP-1 in the siTIMP-1 group was significantly lower than that in the blank group. Compared with the blank group, the cell viability in the TGF-β1 group was significantly improved; the percentage of the cells in G0/G1 phase was significantly raised, the percentages of the cells in S phase and G2/M phase were significantly increased. The content of TNF-α was significantly elevated, the protein expression of α-SMA, collagen-I and β-catenin were significantly enhanced. Compared with TGF-β1 group, after knock-down of TIMP-1 gene expression, the cell viability in siTIMP-1 combined with TGF-β1 group was significantly inhibited, the percentage of the cells in G0/G1 phase was significantly raised, the percentages of the cells in S phase and G2/M phase were significantly decreased, the content of TNF-α was significantly reduced, and the protein expression of α-SMA, collagen-I and α-catenin was significantly depressed. There was no significant difference between the cells transfected with small interfering RNA of negative control and those of TGF-β1 group. Conclusion Knock-down of TIMP-1 gene expression inhibits the cell viability, cell cycle, collagen synthesis and the release of inflammatory factor TNF-α in the MRC-5 cells. The mechanism is related to the inhibition of Wnt/β-catenin signaling pathway." @default.
- W2987996966 created "2019-11-22" @default.
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- W2987996966 date "2019-01-01" @default.
- W2987996966 modified "2023-09-27" @default.
- W2987996966 title "[Knocking down TIMP-1 inhibits the proliferation and collagen synthesis of MRC-5 human embryonic lung fibroblasts induced by TGF-β1]." @default.
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