FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2988132232> ?p ?o ?g. }

• W2988132232 abstract "Abstract Background Ovarian cancer is the leading cause of gynecologic cancer death in the United States despite effective first-line systemic chemotherapy. Cancer stem cells (CSCs) retain the ability to self-renew and proliferate and may be a means of harboring disease that evades standard treatment strategies. We previously performed a high-throughput screen to assess differential protein expression in ovarian CSCs compared to non-CSCs and observed that Thy-1 was more highly expressed in CSCs. Our primary aim was to validate Thy-1 (CD90) as a cancer stem cell (CSC) marker in epithelial ovarian cancer (EOC), correlate with clinical outcomes, and assess as a potential therapeutic target. Results Kaplan Meier (KM) Plotter data were correlated with survival outcomes. Quantitative real-time PCR, flow cytometry, and immunoblots assessed RNA and protein expression. Limiting dilution assays assessed self-renewal capacity and proliferation assays assessed proliferative capacity. RNA in-situ hybridization was performed on patient specimens to assess feasibility. Thy-1 (CD90) is more highly expressed in ovarian CSCs than non-CSCs, in EOC compared to benign ovarian epithelium ( P < 0.001), and is highest in serous EOC ( P < 0.05). Serous ovarian cancers with high Thy-1 expression have poorer outcomes (median PFS 15.8 vs. 18.3 months, P = 0 < 0.001; median OS 40.1 v. 45.8 months, P = 0.036). Endometrioid ovarian cancers with high Thy-1 have poorer PFS, but no difference in OS (upper quartile PFS 34 v. 11 months, P = 0.013; quartile OS not reached, P = 0.69). In vitro, Thy-1 expression is higher in CSCs versus non-CSCs. EOC cells with high Thy-1 expression demonstrate increased proliferation and self-renewal. Thy-1 knockdown in EOC cells decreases proliferative capacity and self-renewal capacity, and knockdown is associated with decreased expression of stem cell transcription factors NANOG and SOX2. RNA in situ hybridization is feasible in ovarian cancer tissue specimens. Conclusions Thy-1 is a marker of ovarian CSCs. Increased expression of Thy-1 in EOC predicts poor prognosis and is associated with increased proliferative and self-renewal capacity. Thy-1 knockdown decreases proliferative and self-renewal capacity, and represents a potential therapeutic target." @default.
• W2988132232 created "2019-11-22" @default.
• W2988132232 creator A5003321703 @default.
• W2988132232 creator A5004589257 @default.
• W2988132232 creator A5005911289 @default.
• W2988132232 creator A5018592399 @default.
• W2988132232 creator A5029411924 @default.
• W2988132232 creator A5053750899 @default.
• W2988132232 creator A5054804282 @default.
• W2988132232 creator A5066089922 @default.
• W2988132232 creator A5067090684 @default.
• W2988132232 creator A5080606232 @default.
• W2988132232 creator A5082400807 @default.
• W2988132232 date "2019-11-17" @default.
• W2988132232 modified "2023-09-23" @default.
• W2988132232 title "Thy-1 predicts poor prognosis and is associated with self-renewal in ovarian cancer" @default.
• W2988132232 cites W1787878270 @default.
• W2988132232 cites W1964736695 @default.
• W2988132232 cites W2004471993 @default.
• W2988132232 cites W2013550974 @default.
• W2988132232 cites W2019719781 @default.
• W2988132232 cites W2020458598 @default.
• W2988132232 cites W2026171286 @default.
• W2988132232 cites W2035943873 @default.
• W2988132232 cites W2044929474 @default.
• W2988132232 cites W2067067209 @default.
• W2988132232 cites W2075404238 @default.
• W2988132232 cites W2076422009 @default.
• W2988132232 cites W2079849392 @default.
• W2988132232 cites W2090996367 @default.
• W2988132232 cites W2094829108 @default.
• W2988132232 cites W2109186855 @default.
• W2988132232 cites W2111042952 @default.
• W2988132232 cites W2118548073 @default.
• W2988132232 cites W2119774224 @default.
• W2988132232 cites W2135947389 @default.
• W2988132232 cites W2139261736 @default.
• W2988132232 cites W2148264387 @default.
• W2988132232 cites W2154175915 @default.
• W2988132232 cites W2166310305 @default.
• W2988132232 cites W2177575632 @default.
• W2988132232 cites W2265169815 @default.
• W2988132232 cites W2341594612 @default.
• W2988132232 cites W2413591396 @default.
• W2988132232 cites W2418287256 @default.
• W2988132232 cites W2547394998 @default.
• W2988132232 cites W2555095864 @default.
• W2988132232 cites W2759934382 @default.
• W2988132232 cites W2772017385 @default.
• W2988132232 cites W2784597821 @default.
• W2988132232 cites W2911188335 @default.
• W2988132232 cites W2929297772 @default.
• W2988132232 cites W2953056120 @default.
• W2988132232 doi "https://doi.org/10.1186/s13048-019-0590-5" @default.
• W2988132232 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6858973" @default.
• W2988132232 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31735168" @default.
• W2988132232 hasPublicationYear "2019" @default.
• W2988132232 type Work @default.
• W2988132232 sameAs 2988132232 @default.
• W2988132232 citedByCount "18" @default.
• W2988132232 countsByYear W29881322322020 @default.
• W2988132232 countsByYear W29881322322022 @default.
• W2988132232 countsByYear W29881322322023 @default.
• W2988132232 crossrefType "journal-article" @default.
• W2988132232 hasAuthorship W2988132232A5003321703 @default.
• W2988132232 hasAuthorship W2988132232A5004589257 @default.
• W2988132232 hasAuthorship W2988132232A5005911289 @default.
• W2988132232 hasAuthorship W2988132232A5018592399 @default.
• W2988132232 hasAuthorship W2988132232A5029411924 @default.
• W2988132232 hasAuthorship W2988132232A5053750899 @default.
• W2988132232 hasAuthorship W2988132232A5054804282 @default.
• W2988132232 hasAuthorship W2988132232A5066089922 @default.
• W2988132232 hasAuthorship W2988132232A5067090684 @default.
• W2988132232 hasAuthorship W2988132232A5080606232 @default.
• W2988132232 hasAuthorship W2988132232A5082400807 @default.
• W2988132232 hasBestOaLocation W29881322321 @default.
• W2988132232 hasConcept C10205521 @default.
• W2988132232 hasConcept C116716535 @default.
• W2988132232 hasConcept C121608353 @default.
• W2988132232 hasConcept C126322002 @default.
• W2988132232 hasConcept C143998085 @default.
• W2988132232 hasConcept C150173356 @default.
• W2988132232 hasConcept C204232928 @default.
• W2988132232 hasConcept C2778560582 @default.
• W2988132232 hasConcept C2780427987 @default.
• W2988132232 hasConcept C2781199814 @default.
• W2988132232 hasConcept C28328180 @default.
• W2988132232 hasConcept C502942594 @default.
• W2988132232 hasConcept C54355233 @default.
• W2988132232 hasConcept C55427017 @default.
• W2988132232 hasConcept C71924100 @default.
• W2988132232 hasConcept C86803240 @default.
• W2988132232 hasConceptScore W2988132232C10205521 @default.
• W2988132232 hasConceptScore W2988132232C116716535 @default.
• W2988132232 hasConceptScore W2988132232C121608353 @default.
• W2988132232 hasConceptScore W2988132232C126322002 @default.
• W2988132232 hasConceptScore W2988132232C143998085 @default.
• W2988132232 hasConceptScore W2988132232C150173356 @default.
• W2988132232 hasConceptScore W2988132232C204232928 @default.
• W2988132232 hasConceptScore W2988132232C2778560582 @default.

• next