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• W2988398004 abstract "Article Figures and data Abstract eLife digest Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Heterochromatin, a highly compact chromatin state characterized by histone H3K9 methylation and HP1 protein binding, silences the underlying DNA and influences the expression of neighboring genes. However, the mechanisms that regulate heterochromatin spreading are not well understood. In this study, we show that the conserved Mst2 histone acetyltransferase complex in fission yeast regulates histone turnover at heterochromatin regions to control heterochromatin spreading and prevents ectopic heterochromatin assembly. The combined loss of Mst2 and the JmjC domain protein Epe1 results in uncontrolled heterochromatin spreading and massive ectopic heterochromatin, leading to severe growth defects due to the inactivation of essential genes. Interestingly, these cells quickly recover by accumulating heterochromatin at genes essential for heterochromatin assembly, leading to their reduced expression to restrain heterochromatin spreading. Our studies discover redundant pathways that control heterochromatin spreading and prevent ectopic heterochromatin assembly and reveal a fast epigenetic adaptation response to changes in heterochromatin landscape. https://doi.org/10.7554/eLife.06179.001 eLife digest The DNA in the nucleus of a cell is wrapped around histone proteins to form a compact structure known as chromatin. Chromatin's structure can control how the genes in DNA are expressed. Loosely packed chromatin contains active genes, whereas densely packed chromatin (also called ‘heterochromatin’) contains silenced genes that are not expressed. The assembly of DNA into heterochromatin needs to be carefully controlled. Otherwise, the DNA next to heterochromatin regions can become densely packed as well (via a process called ‘heterochromatin spreading’), and the genes within this DNA are incorrectly silenced. Incorrect gene silencing is often associated with diseases such as cancer. Cells add chemical groups onto the histone proteins to influence how chromatin is compacted. Densely packed chromatin contains histones with many methyl groups but few acetyl groups. A protein called Epe1, which potentially removes methyl groups, helps to prevent heterochromatin spreading in yeast cells. Wang et al. found that an enzyme called Mst2, which adds acetyl groups onto histones, also limits heterochromatin spreading and prevents extra heterochromatin from assembling at undesirable locations. Wang et al. then generated yeast cells that lacked both Epe1 and Mst2. At first, these cells were sickly and unable to grow, because several essential genes were incorrectly silenced due to rampant heterochromatin spreading. However, the cells quickly overcame this growth defect by gaining an additional mutation. Normally mutations occur through changes in DNA sequences. However, Wang et al. found that the cells acquired this mutation by packing a gene required for heterochromatin assembly into heterochromatin. This in turn stopped more chromatin from becoming packed too densely. Changes to chromatin can also be passed on to the yeast's offspring, and such a change could help the offspring to better cope with changes in heterochromatin levels. Future work could test how often inheritable changes to chromatin modification help organisms adapt to environmental stresses, or if similar changes allow cancer cells to become tolerant to anticancer drugs. https://doi.org/10.7554/eLife.06179.002 Introduction Eukaryotic genomic DNA is folded with histones and non-histone proteins in the form of chromatin, which regulates every aspect of DNA metabolism, including transcription, replication, recombination, and DNA damage repair. Chromatin is classified into euchromatin, which is gene rich and actively transcribed, and heterochromatin, which is gene poor and highly compacted (Grewal and Jia, 2007). Heterochromatin preferentially forms at repetitive DNA elements in order to limit transcription and recombination at these regions to maintain genome integrity. It also forms at developmentally regulated genes to regulate their expression in response to developmental cues and external stimuli. Heterochromatin tends to spread into neighboring regions, leading to the inactivation of genes in a sequence-independent manner (Talbert and Henikoff, 2006; Wang et al., 2014). Therefore, the sites of heterochromatin formation and extent of heterochromatin spreading need to be tightly controlled to prevent improper gene silencing, and misregulation of heterochromatin assembly has been linked to many human diseases, especially various types of cancers (Geutjes et al., 2012). Heterochromatic regions generally have distinct chromatin signatures such as histones that are hypoacetylated and methylated at histone H3 lysine 9 (H3K9me), and the enrichment of HP1 family proteins (Rea et al., 2000; Bannister et al., 2001; Lachner et al., 2001; Nakayama et al., 2001). Formation of heterochromatin requires the concerted actions of a diverse group of histone-modifying proteins, such as H3K9 methyltransferases and histone deacetylases (HDACs), and is divided into three distinct steps: establishment, spreading, and maintenance (Grewal and Moazed, 2003; Rusche et al., 2003). Heterochromatin is established at nucleation centers through the targeting of histone-modifying activities by transcription factors or non-coding RNAs (Cohen and Jia, 2014). Subsequently, heterochromatin spreads into neighboring regions, mostly via a network of interactions among chromatin proteins, resulting in the formation of large heterochromatin domains independent of the underlying DNA sequences (Talbert and Henikoff, 2006; Cohen and Jia, 2014). Once these domains are formed, they can maintain themselves also through interactions among chromatin proteins even in the absence of the initiation signal (Moazed, 2011; Ragunathan et al., 2014). The formation of heterochromatin has been extensively studied in fission yeast, which uses highly conserved histone-modifying enzymes and chromatin proteins for heterochromatin assembly, such as the SUV39 family histone H3K9 methyltransferase Clr4, the HP1 homologue Swi6, and HDACs Sir2 and Clr3 (Grewal and Jia, 2007). There are four types of heterochromatin identified in fission yeast: constitutive heterochromatin at repeat regions such as centromeres, telomeres, and the silent mating-type region (Grewal and Jia, 2007); facultative heterochromatin islands at a subset of meiotic genes (Hiriart et al., 2012; Zofall et al., 2012; Tashiro et al., 2013; Egan et al., 2014); HOODs (heterochromatin domains) at sexual differentiation genes and retrotransposons in response to the misregulation of the exosome (Yamanaka et al., 2013); and transient heterochromatin at convergent genes (Gullerova and Proudfoot, 2008). These locations use distinct pathways to recruit histone-modifying activities to form heterochromatin. The establishment of constitutive heterochromatin at repetitive DNA elements requires the RNA interference (RNAi) pathway, a phenomenon also vastly conserved in eukaryotes (Moazed, 2009; Lejeune and Allshire, 2011; Castel and Martienssen, 2013). The DNA repeats are transcribed and the transcripts are processed by the RNAi machinery into small interfering RNAs (siRNAs), which target the Clr4 complex (CLRC, consisting of Clr4, Cul4, Rik1, Raf1, and Raf2) to repeat regions to initiate H3K9me. In addition, DNA binding factors, such as telomeric shelterin and stress-activated ATF/CREB family proteins Atf1/Pcr1, also directly recruit histone-modifying activities to establish constitutive heterochromatin at telomeres and the silent mating-type region, respectively (Jia et al., 2004; Kim et al., 2004; Kanoh et al., 2005; Tadeo et al., 2013). The formation of facultative heterochromatin islands at meiotic genes requires RNA binding protein Mmi1, Zinc finger protein Red1, and the exosome (Hiriart et al., 2012; Zofall et al., 2012; Tashiro et al., 2013; Egan et al., 2014). Mmi1 binds to RNA transcripts containing DSR (determinant of selective removal) sequences and recruits the RNA-induced transcriptional silencing (RITS) complex and the Red1-Mtl1 complex, which directly interacts with Clr4 complex, to initiate H3K9me at meiotic genes (Harigaya et al., 2006; Zofall et al., 2012; Lee et al., 2013; Egan et al., 2014). HOODs are formed at sexual differentiation genes and retrotransposons in response to exosome malfunction or changes in environmental conditions and requires RNAi, polyA polymerase Pla1, and PolyA binding protein Pab2 (Lee et al., 2013; Yamanaka et al., 2013). Convergent genes generate overlapping transcripts during the G1 phase of the cell cycle, which induce the formation of transient heterochromatin through the RNAi pathway (Gullerova and Proudfoot, 2008). The spreading of heterochromatin requires Swi6 and the chromodomain of Clr4, both of which bind to H3K9me and position Clr4 to methylate neighboring nucleosomes (Hall et al., 2002; Zhang et al., 2008; Al-Sady et al., 2013). The reiteration of H3K9 methylation and recruitment of Clr4 by H3K9me, either directly through the chromodomain or indirectly through Swi6, results in the ‘inch-worm’-like spreading of heterochromatin from nucleation centers into large chromosome domains (Talbert and Henikoff, 2006; Wang et al., 2014). Some heterochromatin regions are flanked by DNA sequences termed boundary elements, which block heterochromatin spreading (Wang et al., 2014). In other cases, heterochromatin borders are determined by the local balance of heterochromatin and euchromatin proteins, which tends to differ between cells. Therefore, precise regulation of heterochromatin spreading is essential to maintain stable gene expression profiles. One of the best-known negative regulators of heterochromatin spreading is Epe1 as epe1∆ results in heterochromatin spreading beyond its normal boundaries as well as ectopic heterochromatin formation (Zofall and Grewal, 2006; Trewick et al., 2007; Zofall et al., 2012; Ragunathan et al., 2014). Loss of Epe1 also bypasses RNAi for pericentric heterochromatin assembly by strengthening heterochromatin spreading (Trewick et al., 2007). Epe1 contains a JmjC domain, which is frequently associated with histone demethylase activity. Although no demethylase activity has been detected for Epe1 (Tsukada et al., 2006), genetic evidence is consistent with Epe1 being a H3K9 demethylase and conserved catalytic residues are essential for Epe1 function (Trewick et al., 2007; Ragunathan et al., 2014). The Mst2 complex is similar in composition to budding yeast NuA3 and mammalian HBO1/MOZ/MORF complexes (Wang et al., 2012). It is a highly specific histone H3K14 acetyltransferase that cooperates with Gcn5 to regulate global H3K14 acetylation levels (Nugent et al., 2010; Wang et al., 2012). The formation of heterochromatin is negatively correlated with H3K14 acetylation (Sugiyama et al., 2007; Motamedi et al., 2008), and mst2∆ bypasses the requirement of the RNAi pathway for pericentric heterochromatin assembly through modulating H3K14ac levels at heterochromatin (Reddy et al., 2011). Moreover, mst2∆ strengthens silencing at telomeres (Gomez et al., 2005). These results suggest that Mst2 complex functions to antagonize heterochromatic silencing, although the mechanism by which it affects heterochromatin assembly is unknown. The ability to bypass RNAi requires ablating the enzymatic activity of the Mst2 complex (Reddy et al., 2011). It was proposed that Mst2-mediated H3K14 acetylation regulates histone turnover at heterochromatin regions and the loss of such activity preserves parental histone modifications to promote heterochromatin maintenance (Reddy et al., 2011), although the ability of Mst2 to regulate histone turnover has not been directly tested. In this study, we show that Mst2 regulates histone turnover at heterochromatin regions and that loss of Mst2 results in heterochromatin spreading at telomeres and heterochromatin islands where boundaries are absent. We also found that mst2∆ epe1∆ cells are initially very sick due to heterochromatin spreading-mediated inactivation of essential genes, suggesting that Mst2 and Epe1 function redundantly in regulating heterochromatin spreading. Interestingly, these cells quickly recover by forming ectopic heterochromatin at the clr4+ locus to mitigate the negative effects of heterochromatin. Disrupting heterochromatin assembly at the clr4+ locus results in ectopic heterochromatin formation at the rik1+ locus, which encodes another subunit of the Clr4 complex required for H3K9me. These results demonstrate that promiscuous heterochromatin assembly generates epigenetic mutations that provide fast adaptions to heterochromatin stress. Results Mst2 regulates histone turnover at heterochromatin To directly examine the role of the Mst2 complex in regulating histone turnover, we generated a Flag-tagged version of histone H3 driven by the urg1 promoter at the endogenous urg1+ locus, which can be quickly induced by the addition of uracil into the growth medium at levels far below the endogenous histone H3 (Watt et al., 2008) (Figure 1A). To prevent replication-dependent histone incorporation, we blocked the cell cycle with hydroxyl urea (HU) before induction of H3-Flag expression (Figure 1B). We found that pericentric dh repeat was associated with lower amounts of H3-Flag in wild-type cells compared with RNAi mutant dcr1∆ (Figure 1C), suggesting that histone turnover rates increase when heterochromatin is compromised. In addition, the incorporation of H3-Flag was reduced in epe1∆ dcr1∆ cells, as observed previously (Figure 1C) (Aygun et al., 2013). In mst2∆ dcr1∆ cells, H3-Flag incorporation was reduced to wild-type levels (Figure 1C), suggesting that the Mst2 complex indeed regulates histone turnover at heterochromatin. Figure 1 with 1 supplement see all Download asset Open asset Mst2 counteracts heterochromatin assembly. (A) Western blot analysis of H3-Flag levels. Samples were taken at indicated times after the addition of uracil, and Western blot analyses were performed with Flag and H3 antibodies. (B) Schematic diagram of the histone turnover assay. (C) Enrichment of H3-Flag at pericentric dh sequence as an indicator of histone turnover rates. The values are normalized to a region within the silent mating-type locus with background histone turnover (Aygun et al., 2013). Error bars represent standard deviation of three experiments. (D) ChIP–chip analyses of H3K9me2 levels across the genome. (E) ChIP–chip data of H3K9me2 levels around the telomere IL, centromere I, the silent mating-type region, and the mei4+ locus. (F) Mst2 is not required for boundary function at IRC1R. Serial dilution analysis were performed to measure the expression of IRC1R::ura4+ reporter. https://doi.org/10.7554/eLife.06179.003 To further examine the role of the Mst2 complex in regulating heterochromatin assembly, we performed Chromatin Immunoprecipitation coupled with DNA microarray (ChIP–chip) analyses of H3K9me2 levels across the fission yeast genome. In wild-type cells, H3K9me2 was mainly present at centromeres, telomeres, and the silent mating-type region (Figure 1D). There were also a few heterochromatic islands with low levels of H3K9me2 (Figure 1D). Although less heterochromatic islands were identified compared to a recent study (Zofall et al., 2012), our results are consistent with that of an earlier one (Cam et al., 2005). The discrepancies might be caused by the use of batches of antibody with different sensitivity or different data processing methods. In mst2∆ cells, constitutive heterochromatin domains at centromeres and the silent mating-type region were in good agreement with wild-type cells, but telomeric heterochromatin showed significant spreading into chromosome arms (Figure 1D,E and Figure 1—figure supplement 1), consistent with previous findings that mst2∆ strengthens silencing at telomeres (Gomez et al., 2005). Interestingly, there are a number of additional small H3K9me2 peaks scattered across the genome, most of which are also present in epe1∆ cells (Figure 1D,E, and Supplementary file 1) (Zofall et al., 2012). Therefore, Mst2 also prevents ectopic heterochromatin assembly, similar to Epe1. We observed only minor heterochromatin spreading in telomeric regions in epe1∆ cells compared with a previous study (Zofall et al., 2012), which might be due to the presence of two epigenetically stable subpopulations of cells with different effects on heterochromatin assembly (Trewick et al., 2007). The difference between pericentric regions, telomeres, and heterochromatin islands is the presence of well-defined boundary elements at pericentric regions that block heterochromatin spreading (Wang et al., 2014). We found that mst2∆ has no effect on boundary activity of an inverted repeat at the pericentric region, IRC1R, which requires Epe1 and the double bromodomain protein Bdf2 for function (Figure 1F) (Wang et al., 2013), suggesting that Mst2 regulates heterochromatin spreading only in the absence of boundaries. Misregulation of heterochromatin affects the fitness of mst2Δ epe1Δ cells Since mst2∆ and epe1∆ have similar phenotypes in heterochromatin assembly and each bypasses the RNAi pathway for pericentric heterochromatin functions (Trewick et al., 2007; Reddy et al., 2011), we generated mst2∆ epe1∆ cells to examine their epistatic relationship. All freshly generated mst2∆ epe1∆ cells formed very small colonies, suggesting a strong negative genetic interaction between these two mutants (Figure 2A and Figure 2—figure supplement 1), consistent with high throughput epistasis mapping (Roguev et al., 2008; Ryan et al., 2012). Moreover, abolishing the enzymatic activity of Mst2 (mst2-E274Q or nto1∆) or Epe1 (epe1-H374A and epe1-Y307A) resulted in similar sickness (Figure 2—figure supplement 1), suggesting that the enzymatic activities of Mst2 and Epe1 have redundant functions. Double mutant of mst2∆ bdf2∆ had no defects in growth (Figure 2—figure supplement 1), suggesting that the boundary activity of Epe1 is not involved in genetic interaction with Mst2. Figure 2 with 2 supplements see all Download asset Open asset A suppressor mutation confers normal growth of mst2∆ epe1∆ cells. (A, D, E, H) Tetrad dissection analysis of the indicated genetic crosses. Pictures are examples of colonies derived from the same tetrad containing all individual genotypes, after one replication for a total of 6 days growth. (B) Serial dilution analysis of indicated strains. Cells were grown in rich medium overnight before dilution analyses were performed. (C) The growth curve of indicated strains. (F) Workflow to introduce mst2∆ and epe1∆ into the deletion library. (G) Left, a representative image of colony growth was shown. Middle, colonies were assigned scores between 0 and 3, as indicated. Right, list of identified heterochromatin mutants that confer fast growth. https://doi.org/10.7554/eLife.06179.005 Interestingly, cells from the small mst2∆ epe1∆ colonies grew comparably to wild-type cells (Figure 2B,C), suggesting that the accumulation of either a genetic or more intriguingly, an epigenetic suppressor leads to quick and persistent production of normally growing mst2∆ epe1∆ cells. We crossed independent clones of recovered mst2∆ epe1∆ cells (mst2∆ epe1∆*, which denotes the presence of a suppressor) and found that all resulting progenies grew normally from the beginning (Figure 2D). Moreover, when we crossed mst2∆ epe1∆* cells with wild-type cells, half of the resulting mst2∆ epe1∆ colonies were small and the other half were normal (Figure 2E), suggesting that changes associated with a single genomic locus was responsible for the recovery of these cells and also ruling out the possibility that the initial growth defects were the result of these cells going through meiosis. To identify the suppressor, we performed two successive rounds of crosses to introduce mst2∆ and epe1∆ into the fission yeast deletion library and measured the initial growth of triple mutants before accumulation of a suppressor (Figure 2F). Interestingly, a number of heterochromatin assembly mutants allowed robust growth of mst2∆ epe1∆ cells. Such mutants included deletions of components of the CLRC histone H3K9 methyltransferase complex (clr4∆, raf1∆, and raf2∆), an HP1 protein (swi6∆), and histone deacetylases (sir2∆ and clr3∆) (Figure 2G and Supplementary file 2). In contrast, none of the RNAi factors were identified in our screen. Individual crosses also confirmed that heterochromatin mutants conferred normal growth to mst2∆ epe1∆ cells (Figure 2H and Figure 2—figure supplement 2). Such data suggest that the effect of mst2∆ epe1∆ on cell growth is possibly the result of misregulation of heterochromatin. Increased heterochromatin spreading is responsible for the initial growth defects of mst2Δ epe1Δ cells To determine whether there are any global changes in heterochromatin organization, we decided to perform ChIP–chip analysis of H3K9me2 levels across the genome in mst2∆ epe1∆ cells. However, the quick generation of epigenetic suppressors in mst2∆ epe1∆* cells prevented us from directly testing the reason for the initial growth defects. Because H3K9me functions upstream of Swi6 localization and the silencing function of H3K9me requires Swi6, we reasoned that examining H3K9me2 levels in mst2∆ epe1∆ swi6∆ cells might show the misregulation of heterochromatin assembly that resembles early stages mst2∆ epe1∆ cells. Indeed, in mst2∆ epe1∆ swi6∆ cells, the H3K9me2 domains at constitutive heterochromatin regions such as centromeres showed significant expansion, even when boundary elements are present (Figure 3A,B). In addition, many additional peaks of H3K9me2 were detected across the genome, at levels comparable to constitutive heterochromatin domains (Figure 3A,B, Figure 3—figure supplement 1, and Supplementary file 1). Most, but not all, of these additional sites correspond to previously described heterochromatin islands. Compared to wild-type, mst2∆, or epe1∆ cells, these heterochromatic islands were also greatly expanded (Figure 3B and Figure 3—figure supplement 1). Given that Swi6 also contributes to heterochromatin spreading (Hall et al., 2002; Al-Sady et al., 2013), heterochromatin probably spreads over even longer distances when Swi6 is present. Interestingly, essential genes reside within or near some of the expanded H3K9me2 domains (Figure 3B and Figure 3—figure supplement 1), suggesting that misregulation of heterochromatin spreading inactivates essential genes and causes the initial sickness of mst2∆ epe1∆ cells. Figure 3 with 1 supplement see all Download asset Open asset Increased heterochromatin spreading in mst2∆ epe1∆ cells leads to growth defects. (A) ChIP–chip analyses of H3K9me2 levels across the genome. (B) ChIP–chip data of H3K9me2 levels at centromere I, mei4+, mcp7+, and clr4+ locus. (C) Tetrad dissection analysis of the indicated genetic cross. (D) ChIP-qPCR analysis of H3K9me2 levels at indicated locations, normalized against act1+. Error bars represent standard deviation of three experiments. https://doi.org/10.7554/eLife.06179.008 If heterochromatin spreading is the cause of the initial growth defects of mst2∆ epe1∆ cells, we expect that mutations blocking heterochromatin spreading will abolish such an effect. The spreading of heterochromatin requires Swi6 as well as the chromodomain of Clr4, which binds to pre-existing H3K9me to allow the modification of adjacent nucleosomes (Hall et al., 2002; Zhang et al., 2008; Al-Sady et al., 2013). Our data that mst2∆ epe1∆ swi6∆ allows normal growth (Figure 2H) is consistent with such a hypothesis. However, Swi6 is required for heterochromatin spreading as well as heterochromatin-mediated silencing, making it difficult to definitively assess the contribution of heterochromatin spreading in this process. We therefore tested a mutation within the Clr4 chromodomain, W31G, which affects the binding of Clr4 to H3K9me to block heterochromatin spreading (Zhang et al., 2008). Indeed, mst2∆ epe1∆ clr4-W31G cells showed no initial growth defects (Figure 3C) and heterochromatin expansion is prevented as indicated by ChIP analysis of H3K9me2 levels outside of centromere I boundary and at the mei4+ locus (Figure 3D). Ectopic heterochromatin at the clr4+ locus promotes adaption of mst2Δ epe1Δ cells ChIP–chip analysis also showed that the patterns and levels of H3K9me2 in mst2∆ epe1∆* cells were more similar to those in wild-type cells with high levels of H3K9me2 at constitutive heterochromatic regions and low levels of H3K9me2 at heterochromatic islands, and much less heterochromatin spreading compared with mst2∆ epe1∆ swi6∆ cells (Figure 3A,B). Most significantly, H3K9me2 was enriched at a 5 kilobase region covering clr4+ and an adjacent gene meu6+, at levels comparable to constitutive heterochromatin regions in independent clones tested (Figures 3A,B, and 4A, and data not shown). Consistent with the fact that H3K9me is associated with gene silencing, both clr4+ mRNA and Clr4 protein levels were reduced in mst2∆ epe1∆* cells (Figure 4B,C). Figure 4 Download asset Open asset Inheritance of ectopic heterochromatin at the meu6-clr4 locus. (A, E) ChIP-qPCR analysis of H3K9me2 levels at the clr4+ coding region, normalized against act1+. Error bars represent standard deviation of three experiments. (B, F) qRT-PCR analysis of clr4+ mRNA levels, normalized against act1+. Error bars represent standard deviation of three experiments. (C) Western blot analyses of Flag-Clr4 and Tubulin protein levels. (D) Tetrad dissection analysis of indicated genetic crosses. (G) Serial dilution analysis to measure the expression of otr::ura4+ reporter. https://doi.org/10.7554/eLife.06179.010 To test if the epigenetically silenced clr4+ locus is the suppressor of mst2∆ epe1∆* cells, we crossed mst2∆ epe1∆* (containing a methylated clr4+ locus) with Flag-clr4+ cells (containing an unmethylated Flag-clr4+ locus). We found that the resulting mst2∆ epe1∆ clr4+ daughters (inheriting the methylated clr4+ locus) grew normally, whereas the mst2∆ epe1∆ Flag-clr4+ daughters (inheriting the unmethylated Flag-clr4+ locus) were sick (Figure 4D). Moreover, ChIP analysis showed that the mst2∆ epe1∆ clr4+ progeny also inherited H3K9me2 associated with this locus (Figure 4E), and clr4+ mRNA levels were low (Figure 4F). Therefore, the inheritance of a silenced clr4+ locus allows cells to avoid the negative effects on cell growth imposed by mst2∆ epe1∆. In contrast, wild-type cells inheriting the methylated clr4+ locus lost H3K9me2, clr4+ mRNA levels were significantly restored, and these cells exhibited no defects in the silencing of a pericentric otr::ura4+ reporter gene (Figure 4E,F,G). These results suggest that the silencing of clr4+ is epigenetic and not due to changes in DNA sequence. They also suggest that the continued absence of Mst2 and Epe1 is required to maintain H3K9me2 at the clr4+ locus. Sequences 3′ to clr4+ is required for heterochromatin assembly at the clr4+ locus in mst2Δ epe1Δ cells We then examined whether any of the known heterochromatin assembly pathways are required for heterochromatin assembly at the clr4+ locus in mst2∆ epe1∆ cells. We found that mst2∆ epe1∆ dcr1∆ cells also quickly recovered and H3K9me2 levels were similar at the clr4+ locus in mst2∆ epe1∆* and mst2∆ epe1∆ dcr1∆* cells, suggesting that RNAi is not required for heterochromatin assembly at clr4+, even though clr4+ is in a convergent orientation with meu6+ (Figure 5—figure supplement 1). In addition, H3K9me2 levels persisted in mst2∆ epe1∆ mmi1∆* and mst2∆ epe1∆ pab2∆* cells (Figure 5—figure supplement 1), suggesting that heterochromatin assembly is not through Mmi1-mediated facultative heterochromatin assembly pathway or Pab2-mediated assembly of HOOD, even though meu6+ is a meiotic gene. Therefore, heterochromatin assembly at clr4+ differs from known heterochromatin assembly pathways. Due to the severe growth defects associated with red1∆ or rrp6∆, we were unable to generate triple mutant strains with mst2∆ epe1∆ and whether these factors are involved in H3K9me2 at the clr4+ locus in mst2∆ epe1∆ cells is unknown. The domain of H3K9me2 in mst2∆ epe1∆* cells includes clr4+ and meu6+, with its center within meu6+ coding region. Interestingly, RNA sequencing analysis showed that meu6+ is not expressed in vegetative growing cells and clr4+ transcript runs through the entire meu6+ gene (Figure 5A) (Wang et al., 2015). We therefore replaced the entire meu6+ open reading frame with a kanMX6 cassette, in the same transcription orientation as meu6+ (Figure 5B). Given that meu6+ is right next to clr4+, we first examined whether this manipulation affects Clr4 function. We found that meu6∆::kanMX6 has no silencing defects at otr::ura4+, and clr4+ mRNA and Clr4 protein levels were similar to those of wild-type cells (Figure 5—figure supplement 2). ChIP analysis showed that H3K9me2 was abolished at the clr4+ locus in mst2∆ epe1∆ meu6∆* cells (Figure 5C), and clr4+ mRNA and Clr4 protein levels were similar to wild-type cells (Figure 5D,E). We also generated a meu6-Flag::KanMX6 strain, which preserves the coding sequence of meu6+, and found that H3K9me2 levels at the clr4+ locus were also abolished in mst2∆ epe1∆ meu6-Flag::KanMX6 cells (Figure 5—figure supplement 3), suggesting that the m" @default.
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• W2988398004 title "Author response: Rapid epigenetic adaptation to uncontrolled heterochromatin spreading" @default.
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