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• W2988597817 startingPage "159" @default.
• W2988597817 abstract "Directed evolution is a proven approach to fine tune or modify biomolecules for various applications ranging from research to industry. The process of evolution requires methods that are capable of not only generating genetic diversity but also to distinguish the variants of desired characteristics. One method that is synonymous with directed evolution of proteins is phage display. Here, we present a protocol describing the application of magnetic nanoparticles coupled with a processor to carry out the identification of monoclonal antibodies (mAbs) from a diverse antibody library via phage display. Target antigens are coupled to magnetic nanoparticles as the solid phase for the isolation of the binding mAbs via affinity. A gradual enrichment in clones would result in increasing ELISA readouts with increasing rounds of panning. During monoclonal level analysis, positivity can be deduced with comparison to background and controls. The biopanning process can also be adopted for the directed evolution of enzymes, scaffold proteins or even peptides." @default.
• W2988597817 created "2019-11-22" @default.
• W2988597817 creator A5070776647 @default.
• W2988597817 creator A5082041539 @default.
• W2988597817 creator A5083891437 @default.
• W2988597817 date "2020-01-01" @default.
• W2988597817 modified "2023-10-17" @default.
• W2988597817 title "Magnetic bead-based semi-automated phage display panning strategy for the directed evolution of antibodies" @default.
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• W2988597817 doi "https://doi.org/10.1016/bs.mie.2019.10.023" @default.
• W2988597817 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31931984" @default.
• W2988597817 hasPublicationYear "2020" @default.
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