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• W2988604899 abstract "Abstract In this work, using DNA and exonuclease-I (Exo-I) as signal amplification strategy, a novel and facile electrochemical aptasensor was constructed for fumonisin B 1 (FB 1 ) detection. The G-rich complementary DNA (cDNA) was immobilized onto the electrode surface. Then, aptamer of FB 1 was hybridized with cDNA to form double-stranded DNA. In the absence of FB 1 , double-stranded DNA and G-rich cDNA on the electrode surface promoted effectively methylene blue (MB) enrichment and amplified the initial electrochemical response. In the presence of FB 1 , the combination of aptamer and FB 1 led to the release of aptamer from the electrode surface and the expose of 3′ end of single-stranded cDNA. When Exo-I was added onto the electrode surface, the single-stranded cDNA was degraded in the 3′–5′ direction. The decrease of double-stranded DNA and G-rich cDNA resulted in the less access of MB to the electrode surface, which decreased the electrochemical signal. The experimental conditions including incubation time of FB 1 , the amount of Exo-I and incubation time of Exo-I were optimized. Under the optimal conditions, the linear relationship between the change of peak current and the logarithmic concentration of FB 1 was observed in the range of 1.0 × 10 −3 –1000 ng mL −1 with a low limit of detection of 0.15 pg mL −1 . The experimental results showed that the prepared aptasensor had acceptable specificity, reproducibility, repeatability and stability. Therefore, this proposed aptasensor has a potential application in the food safety detection." @default.
• W2988604899 created "2019-11-22" @default.
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• W2988604899 date "2019-11-09" @default.
• W2988604899 modified "2023-10-10" @default.
• W2988604899 title "A novel electrochemical aptasensor for fumonisin B1 determination using DNA and exonuclease-I as signal amplification strategy" @default.
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• W2988604899 doi "https://doi.org/10.1186/s13065-019-0646-z" @default.
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