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- W2988685902 abstract "A sensitive electrochemiluminescent immunoassay for alkaline phosphatase (ALP) using p-nitrophenyl phosphate (PNPP) as substrate based on the electrochemiluminescence resonance energy transfer (ECRET) is developed. Luminol-doped silica nanoparticles (luminol-SiNPs) are prepared by water/oil (W/O) microemulsion method. PNPP convertes to p-nitrophenol (PNP) in the presence of ALP, which results in the absorption peak shifting from 360 nm to 450 nm. Herein the spectral overlap between absorption spectrum of PNP and electrochemiluminescence (ECL) spectrum of luminol-SiNPs (425 nm) makes energy transfer occur from luminol-SiNPs to PNP. In the optimized conditions, a linear relationship was obtained using this ECRET method at the concentration of ALP from 5 to 50 U/L (r = 0.9905) and with the limit of detection (LOD) of 0.8 U/L. This ECRET method exhibits sufficient specificity for ALP over other enzymes such as horseradish peroxidase, trypsin and lysozyme. • Electrochemiluminescence resonance energy transfer (ECRET) immunoassay for alkaline phosphatase from 5 to 50 U/L. • Spectral overlap between absorption spectrum of p-nitrophenol (PNP) and ECL spectrum of luminol-SiNPs. • ECRET occurs from luminol-SiNPs to PNP and makes electrochemiluminescence intensity be quenched. • ALP converts p-nitrophenyl phosphate (PNPP) to PNP and results the absorption peak to shift from 360 nm to 450 nm." @default.
- W2988685902 created "2019-11-22" @default.
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- W2988685902 date "2020-02-01" @default.
- W2988685902 modified "2023-10-18" @default.
- W2988685902 title "Electrochemiluminescence resonance energy transfer immunoassay for alkaline phosphatase using p-nitrophenyl phosphate as substrate" @default.
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- W2988685902 doi "https://doi.org/10.1016/j.aca.2019.10.073" @default.
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